Corneal transplantation for inflammatory keratopathies in the horse: Visual outcome in 206 cases (1993–2007)

Brooks, D.; Plummer, Caryn E.; Källberg, M. E.; Barrie, K. P.; Ollivier, Franck J.; Hendrix, Diane V. H.; Baker, Ann Sullivan; Scotty, Nicole C.; Utter, Mary; Blackwood, Sarah E.; Nunnery, Catherine; Ben‐Shlomo, Gil; Gelatt, Kirk N.

doi:10.1111/j.1463-5224.2008.00611.x

Cited by 51 publications

(127 citation statements)

References 24 publications

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“…Graft rejection is manifested by a loss of graft transparency after an initial time period of graft clarity (approximately 2 weeks in people and several days in horses) [33,36]. Corneal vascularization, inflammation, and/or infection in the host species prior to transplantation significantly increases the chance of corneal graft rejection or failure [36].…”

Section: Corneal Transplantationmentioning

confidence: 99%

“…In stark contrast to physician ophthalmology, corneal neovascularization is frequently a desired healing response since vascularization substantially improves the patient's ability to fight off corneal infection. Thus, while the vast majority of the corneal transplants performed in veterinary ophthalmology undergo "rejection" by physician standards, vascularization of the donor cornea is considered an optimal progression in wound healing in the majority of cases [32,33].…”

Section: Corneal Transplantationmentioning

confidence: 99%

“…Third, medicallegal implication/permission of harvesting fresh, healthy donor cornea from a euthanized dog has not been established. Presently, veterinary ophthalmologists use frozen corneal transplants to provide tectonic support rather than optical clarity, as corneal endothelial cells do not survive the freezing process [32][33][34]. Common ocular conditions that may be treated with a previously frozen corneal transplant include descemetoceles, stromal abscesses, inflammatory keratitis with or without malacia, corneal perforation/iris prolapse (Figure2), and feline corneal sequestra [32,34,35].…”

Section: Corneal Transplantationmentioning

confidence: 99%

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Corneal Gene Therapy in Veterinary Medicine: A Review

Bosiack¹

2013

J Veterinar Sci Technol

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The cornea is an ideal target for gene therapy due to its accessibility, immune-privileged nature, and ability to perform frequent non-invasive assessment. Gene therapy has been studied in animal models of ocular corneal graft rejection, neovascularization, wound healing, fibrosis, macular degeneration, optic neuropathy, and retinal degeneration. Medical research has widely used animal models in the pursuit of improved therapeutic strategies intended to aid people. In this review we summarize the vectors currently used in ocular gene therapy and the potential applications of emerging new strategies for use in veterinary medicine. diseases. The cornea represents one of the most evolutionary conserved anatomic components across mammals with many shared pathological processes in various species. Corneal gene therapy is therefore an ideal target for collaborative biomedical research. Corneal Gene Therapy in Veterinary MedicineThe potential therapeutic applications of gene therapy are vast. A major advantage of gene therapy over the use of conventional drugs is the prospect of curing disease rather than providing transient relief by suppression of disease symptoms. Replacing defective genes with functional genes through the use of gene therapy offers the prospect for long-term therapeutic benefits without repeated drug application. Successful vision restoration through the use of gene therapy in patients affected with LCA has demonstrated the ability of gene therapy to potentially cure ocular disease and prevent long-term blindness [3,4]. Further research in gene therapy is needed to develop treatments for other vision-impairing ocular diseases.The cornea is an ideal target for gene therapy due to its accessibility, immune-privileged nature, and ability to perform frequent noninvasive assessment [5,6]. Gene therapy has been studied in animal models of ocular corneal graft rejection, neovascularization, wound healing, fibrosis, macular degeneration, optic neuropathy, and retinal degeneration [6][7][8][9][10][11][12][13][14]. The success of corneal gene therapy depends on the type of vector, the extent of therapeutic gene expression, and adverse immune responses [15]. Vectors Conventional vectorsSeveral viral as well as non-viral vectors have been developed to transport genetic material into cells, each of which has advantages and limitations (Table 1) Several studies have assessed the efficacy of Adenovirus (AV) vectors for corneal gene therapy [6]. AV vectors have been shown to effectively deliver genes to the rodent cornea; however, they provide short-term transgene expression and stimulate moderate to severe inflammation [16,17]. Retroviral vectors can successfully deliver genes into the cornea but are not capable of transducing non-dividing cells [18]. Like adenovirus, retroviral vectors have also been shown to stimulate mild-to-severe inflammatory responses and have oncogenic potential due to their integration near proto-oncogenes, making them undesirable [6,19]. Lentiviral vectors appear to be safer tha...

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Section: Corneal Transplantationmentioning

confidence: 99%

Section: Corneal Transplantationmentioning

confidence: 99%

Section: Corneal Transplantationmentioning

confidence: 99%

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Corneal Gene Therapy in Veterinary Medicine: A Review

Bosiack¹

2013

J Veterinar Sci Technol

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“…Corneal transplantation is a routinely performed procedure and is considered the most widely practiced form of organ transplantation in humans. In animals other than horses, the practice is less frequent (Brooks et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Corneal transplantation may be a useful method to manage these abnormalities and the surgeries can be performed for optical, therapeutic, tectonic or cosmetic purposes (Brooks et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Microbiological profile of donor corneas stored for tectonic transplantation purposes in rabbits

Kobashigawa

Martins

Massoli

et al. 2013

Arq. Bras. Med. Vet. Zootec.

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This study aimed to evaluate the microbiota of donor rabbit corneas stored for tectonic transplantation purposes. Swabs from both corneas of 20 rabbits were carefully collected and submitted to microorganism isolation and identification. After this first swab collection, rabbits were euthanized for reasons other than this project and the eyes were enucleated. The corneas were collected and stored to compose the cornea tissue bank. Corneas were stored in a 0.3% tobramycin solution at -20°C. After 30 days, the corneas were thawed at room temperature and removed from the antibiotic. New swabs were obtained from the corneas and submitted to microorganism isolation and identification. Gram positive organisms were predominant in the rabbit corneal flora before storage and the Staphylococcus sp. was the most common microorganism isolated from those samples. No growth was observed on the samples collected after storage. The methods used for collection and storage of the corneas were efficient to constitute a sterile donor corneal tissue bank.Keywords: rabbit, corneal bank, microbiota, corneal transplant RESUMO Analisaram-se córneas armazenadas para transplantes tectônicos usando-se suabes coletados de 20 coelhos, visando ao isolamento e à identificação de microrganismos. Após a coleta das amostras

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