Corneal Ulcer Due to Listeria monocytogenes

Holland, Simon; Alfonso, Eduardo C.; Gelender, Henry; Heidemann, David G.; Mendelsohn, Alan D.; Ullman, Saul; Miller, Darlene

doi:10.1097/00003226-198706020-00008

Cited by 15 publications

(8 citation statements)

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“…The systemic disease it causes is termed listeriosis and it has a multitude of diagnostic manifestations such as miscarriage, muscle pain, stillbirth [4], meningitis [5], septicemia [2], pneumonia [6], corneal ulcers [7], fever, and gastroenteritis [8] in patients. In large outbreaks it has among the highest mortality rates (20%–25%) as compared to other foodborne pathogens reported by the Centers for Disease Control and Prevention [9].…”

Section: Introductionmentioning

confidence: 99%

RT-qPCR Analysis of 15 Genes Encoding Putative Surface Proteins Involved in Adherence of Listeria monocytogenes

Tiong

Muriana

2016

Pathogens

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L. monocytogenes adherence to food-associated abiotic surfaces and the development of biofilms as one of the underlying reasons for the contamination of ready-to-eat products is well known. The over-expression of internalins that improves adherence has been noted in cells growing as attached cells or at elevated incubation temperatures. However, the role of other internalin-independent surface proteins as adhesins has been uncharacterized to date. Using two strains each of weakly- and strongly-adherent L. monocytogenes as platforms for temperature-dependent adherence assays and targeted mRNA analyses, these observations (i.e., sessile- and/or temperature-dependent gene expression) were further investigated. Microplate fluorescence assays of both surface-adherent strains exhibited significant (P < 0.05) adherence at higher incubation temperature (42 °C). Of the 15 genes selected for RT-qPCR, at least ten gene transcripts recovered from cells (weakly-adherent strain CW35, strongly-adherent strain 99-38) subject to various growth conditions were over expressed [planktonic/30 °C (10), sessile/30 °C (12), planktonic/42 °C (10)] compared to their internal control (16SrRNA transcripts). Of four genes overexpressed in all three conditions tested, three and one were implicated as virulence factors and unknown function, respectively. PCR analysis of six unexpressed genes revealed that CW35 possessed an altered genome. The results suggest the presence of other internalin-independent adhesins (induced by growth temperature and/or substratum) and that a group of suspect protein members are worthy of further analysis for their potential role as surface adhesins. Analysis of the molecular basis of adherence properties of isolates of L. monocytogenes from food-associated facilities may help identify sanitation regimens to prevent cell attachment and biofilm formation on abiotic surfaces that could play a role in reducing foodborne illness resulting from Listeria biofilms.

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Section: Introductionmentioning

confidence: 99%

RT-qPCR Analysis of 15 Genes Encoding Putative Surface Proteins Involved in Adherence of Listeria monocytogenes

Tiong

Muriana

2016

Pathogens

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“…Listeria monocytogenes, a Gram-positive nonsporulating motile rod, is one of the most important human pathogens and causes severe diseases such as listeriosis (Farber & Peterkin, 1991). The manifestations of listeriosis include septicaemia, meningitis (Gray, 1962), encephalitis (Armstrong & Fung, 1993), corneal ulcers (Holland et al, 1987), pneumonia (Whitelock-Jones et al, 1989) and intrauterine or cervical infections in pregnant women resulting in spontaneous abortion or stillbirth (Rabau & David, 1962). Similarly to the case in other pathogenic bacteria, acquisition of iron by L. monocytogenes is indispensable for its viability and pathogenicity (Newton et al, 2005;Sword, 1966;Conte et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Structural and functional characterization of an Isd-type haem-degradation enzyme from Listeria monocytogenes

Duong

Park

Kim

et al. 2014

Acta Cryst D Biol Crystallogr

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Bacterial pathogens have evolved diverse types of efficient machinery to acquire haem, the most abundant source of iron in the human body, and degrade it for the utilization of iron. Gram-positive bacteria commonly encode IsdG-family proteins as haem-degrading monooxygenases. Listeria monocytogenes is predicted to possess an IsdG-type protein (Lmo2213), but the residues involved in haem monooxygenase activity are not well conserved and there is an extra N-terminal domain in Lmo2213. Therefore, its function and mechanism of action cannot be predicted. In this study, the crystal structure of Lmo2213 was determined at 1.75 Å resolution and its haem-binding and haem-degradation activities were confirmed. Structure-based mutational and functional assays of this protein, designated as an Isd-type L. monocytogenes haem-degrading enzyme (Isd-LmHde), identified that Glu71, Tyr87 and Trp129 play important roles in haem degradation and that the N-terminal domain is also critical for its haem-degrading activity. The haem-degradation product of Isd-LmHde is verified to be biliverdin, which is also known to be the degradation product of other bacterial haem oxygenases. This study, the first structural and functional report of the haem-degradation system in L. monocytogenes, sheds light on the concealed haem-utilization system in this life-threatening human pathogen.

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“…We entertained the possibility of misdiagnosis as mycobacteria and Listeria monocytogenes have reportedly been mistaken for diphtheroids on gram stains; however, we believe that misdiagnosis is unlikely because we screened with acid-fast staining and Lö wenstein-Jensen culture media, which are expected to rule out these 2 etiologies. 35,36 Although diphtheroids are uncommon as pathogens, the ophthalmic literature indicates they have been isolated in patients with preexisting ocular conditions. 37 It is possible that transient LASIK-induced changes in the ocular surface predisposed this patient to diphtheroid infection.…”

Section: Discussionmentioning

confidence: 99%

Infectious and noninfectious keratitis after laser in situ keratomileusis

Moshirfar¹,

Welling²,

Feiz³

et al. 2007

Journal of Cataract and Refractive Surgery

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The occurrence of post-LASIK keratitis was 2.66%, with DLK being the most common diagnosis overall. The occurrence of noninfectious keratitis (2.34%) was 7.5 times greater than the occurrence of infectious keratitis (0.31%). Adenoviral keratitis had the best visual outcomes overall, with all 18 patients achieving 20/20 BSCVA. In contrast, all 5 eyes with HSV keratitis lost 1 or 2 lines of BSCVA. Excluding adenoviral keratitis, infectious etiologies had significantly worse visual outcomes than noninfectious etiologies at the 20/40 and 20/20 levels (P = .0013 and P<.001, respectively).

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Corneal Ulcer Due to Listeria monocytogenes

Cited by 15 publications

References 0 publications

RT-qPCR Analysis of 15 Genes Encoding Putative Surface Proteins Involved in Adherence of Listeria monocytogenes

RT-qPCR Analysis of 15 Genes Encoding Putative Surface Proteins Involved in Adherence of Listeria monocytogenes

Structural and functional characterization of an Isd-type haem-degradation enzyme from Listeria monocytogenes

Infectious and noninfectious keratitis after laser in situ keratomileusis

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