Corneal Wound Healing Promoted by 3 Blood Derivatives

Abstract

All 3 blood derivatives studied are promoters of corneal reepithelization. However, the corneal wound-healing progresses differently with each derivative, being faster in vitro under AS and s-PRGF treatment and producing in vivo the greatest decrease in wound size under s-PRGF treatment. These findings highlight that the manufacturing process of the blood derivatives may modulate the efficacy of the final product.

Treatment of severe chronic ocular graft-versus-host disease using 100% autologous serum eye drops from a sealed manufacturing system: a retrospective cohort study

“…However, no head-to-head study has been performed to compare efficacy of the different concentrations in vivo. In vitro studies were performed using different ASED concentrations (range: 3.125%–100%) on proliferation and migration of immortalised human corneal epithelial cells13 24 or rabbit corneal epithelial cells 24. Here a concentration of 12.5% ASED was demonstrated for optimal proliferation and 100% ASED for optimal migration,13 whereas 100% ASED was shown to be as equally effective as serum derived from plasma rich in growth factors 24.…”

“…In vitro studies were performed using different ASED concentrations (range: 3.125%–100%) on proliferation and migration of immortalised human corneal epithelial cells13 24 or rabbit corneal epithelial cells 24. Here a concentration of 12.5% ASED was demonstrated for optimal proliferation and 100% ASED for optimal migration,13 whereas 100% ASED was shown to be as equally effective as serum derived from plasma rich in growth factors 24. No reports were available on using 100% ASED, probably due to the assumption of negative effects caused by serum-derived autoantibodies that would be deliberately applied to the ocular surface.…”

Stability of limbal stem cell deficiency after mechanical and thermal injuries in mice

“…The newly regenerated epithelium is anchored firmly to the underlying tissue and the new basement membrane (Dua et al, 1994). This process is mediated by growth factors, adhesion proteins, proteases, cytokines, and other factors provided in part by the limbal blood supply, tear film, stromal and immune cells (Freire et al, 2014). Interactive cross-talk between surrounding cells, the extracellular matrix, and soluble signals are critical for epithelial homeostasis and the ocular surface wound healing response (Dua et al, 2003; Gipson, 1989).…”

Searching for the best blood-derived eye drops

“…5 Last but not least, there are other issues to be considered, including the drawback of not using a standardized ready-to-use product, the large amount of fingerpricks delivered (448 fingerpricks in 8 weeks), and the variability of capillary blood. 6 We strongly encourage the authors to further deepen research in the FAB mechanisms of action and product composition in order to optimize the treatment and improve the quality of life of DES patients, thus offering a fully characterized product.…”