CORO6 Promotes Cell Growth and Invasion of Clear Cell Renal Cell Carcinoma via Activation of WNT Signaling

Wang, Xinjun; Xiao, Yiming; Li, Si; Yan, Zhijian; Luo, Guangcheng

doi:10.3389/fcell.2021.647301

Cited by 4 publications

(4 citation statements)

References 26 publications

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“…Additionally, MMP-9 is one of the Wnt pathway target genes, which can be transcriptionally regulated [ 31 ]. According to some substantial studies, the WNT/ β -catenin pathway is hyperactivated in ccRCC, and targeted inhibition of the WNT/ β -catenin pathway may become a feasible therapeutic strategy for tumors [ 32 – 34 ]. Here, our results showed that NEK2 silencing could obviously downregulate the protein expression levels of key proteins, such as p-GSK-3 β , β -catenin, and the downstream regulator c-Myc, proving that NEK2 silencing could inhibit the WNT/ β -catenin signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

NEK2 Serves as a Novel Biomarker and Enhances the Tumorigenicity of Clear-CellRenal-Cell Carcinoma by Activating WNT/β-Catenin Pathway

Zhou

Lai

Cheng

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Objective. Currently, cumulative evidence has shown that loss of NEK2 function suppresses tumor growth. However, complete studies on the regulatory role of NEK2 in clear-cellrenal-cell carcinoma (ccRCC) are rarely reported. Methods. The GEPIA database was used for information mining to analyze the gene expression differences between ccRCC tumor and normal tissues. At the same time, we analyzed the protein expression of NEK2 in clinical ccRCC samples and ccRCC cell lines. We detected the effect of NEK2 on the biological behavior of ccRCC at the cell level and further verified the biological effect of NEK2 on ccRCC cells in vivo by nude mouse tumorigenesis experiment. The expression of WNT/β-cateninpathway-related proteins and downstream proteins related to cell function were detected by Western blotting. Results. Using the GEPIA database, we observed that NEK2 expression level in ccRCC tissues was significantly higher than that in normal kidney tissues and was also related to tumor grade. The survival time of patients with ccRCC with high NEK2 expression was shorter than that of patients with low NEK2 expression. Compared with adjacent carcinoma and normal renal tubular epithelial cells, NEK2 levels were highly expressed in ccRCC tissues and ccRCC cell lines. NEK2 interference restrained ccRCC cell growth, migration, and invasion. NEK2 regulated the malignant behavior of ccRCC cells through the WNT/β-catenin pathway. Nude mouse tumorigenesis assay results showed that the transplanted tumors from NEK2 silenced mice grew more slowly and were smaller in size than those from control mice. Conclusions. NEK2 elevation may be associated with poor prognosis in ccRCC, and NEK2 enhances ccRCC cell proliferation, migration, and invasion ability by activating the WNT/β-catenin signaling pathway.

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Section: Discussionmentioning

confidence: 99%

NEK2 Serves as a Novel Biomarker and Enhances the Tumorigenicity of Clear-CellRenal-Cell Carcinoma by Activating WNT/β-Catenin Pathway

Zhou

Lai

Cheng

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…The up-regulation of WNT was observed in the progression of breast tumors and was associated with tumor drug resistance [ 43 ]. Wang et al [ 44 ] found that activating WNT signaling can promote cell growth and invasion of ccRCC. Colic et al [ 45 ] found that abnormal MAPK signaling transduction can provide therapeutic potential for the treatment of ovarian cancer.…”

Section: Discussionmentioning

confidence: 99%

Highly expressed of BID indicates poor prognosis and mediates different tumor microenvironment characteristics in clear cell renal cell carcinoma

Zeng,

Ke,

Tian

et al. 2024

Discov Onc

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Background Studies have found that BH3 interacting domain death agonist (BID) is closely related to the occurrence and development of many kinds of tumors. However, little attention has been paid to the situation of BID in clear cell renal cell carcinoma (ccRCC). So, our aim was to explore the effect of BID in ccRCC. Methods Survival analysis, ROC curve, correlation analysis and Cox regression analysis were executed to analyze the prognostic value and clinical correlation of BID in ccRCC. The risk prognosis model was constructed in the training cohort and further validated in the internal testing cohort, ICGC cohort, and GEO cohort. Transcriptome sequencing and immunohistochemical staining of clinical specimens were used to validate the results of bioinformatics analysis. The GSEA, ESTIMATE algorithm, CIBERSORT algorithm, ssGSEA, TIDE score, correlation and difference analysis were used to analyze the effects of BID on immune infiltration in tumor microenvironment (TME). Results BID was highly expressed in ccRCC tissues, which was verified by transcriptome sequencing and immunohistochemical staining of clinical specimens. Patients with high expression of BID had a worse prognosis. BID is an independent prognostic factor for ccRCC. The prognostic model based on BID can accurately predict the prognosis of patients in different cohorts. In addition, the expression levels of BID was closely related to immunomodulatory molecules such as PD-1, LAG3, and CTLA4. Enrichment analysis indicated that BID was significantly enriched in immune-related responses and cancer-related pathways. The change of BID expression mediates different characteristics of immune infiltration in TME. Conclusions BID is highly expressed in ccRCC, which is a reliable biomarker of ccRCC prognosis. It is closely related to TME, and may be a potential target for immunotherapy in patients with ccRCC.

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“…The salient characteristics of EMT is epithelial markers E-cadherin expression quantity, at the same time N-cadherin interstitial cell marker protein expression and transcription factors related to the rise. EMT can through different signaling pathways such as Notch and Wnt pathways activated, these molecular pathways within cells by transcription factor q and Slug control to induce the occurrence of EMT ( 29 , 30 ). The results showed that ligustrazine could significantly decrease the protein expression of N-cadherin, snail and Slug.…”

Section: Discussionmentioning

confidence: 99%

Ligustrazine Inhibits the Migration and Invasion of Renal Cell Carcinoma

Zhang

Wang²,

Cao³

et al. 2023

jkcvhl

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Ligustrazine is a Chinese herb (Chuanxiong) approved for use as a medical drug in China. Recent evidence suggests that ligustrazine has promising antitumor properties. Our preliminary results showed that ligustrazine could inhibit the growth of human renal cell carcinoma (RCC) cell lines. However, the complicated molecular mechanism has not been fully revealed. Therefore, the purpose of this study to investigate the mechanism of ligustrazine resistance in human RCC cells. Cell proliferation, migration, invasion, and colony-formation ability of RCC cells A498 were detected by MTT assay, clonal formation rates, and transwell chamber assay in vitro. The expression of epithelial–mesenchymal transition (EMT)–related proteins were analyzed using western blot test. The effect of ligustrazine on the growth of A498 cells in nude mice was investigated in vivo. Our results showed that ligustrazine could significantly inhibit the proliferation, migration, and invasion of A498 both in vivo and vitro. Western blot analysis showed that the expressions of EMT-related, N-cadherin, snail, and slug proteins were significantly decreased in A498 in the ligustrazine treatment group. This study indicated that ligustrazine could significantly inhibit the malignant biological behaviors of RCC cell lines, possibly by inhibiting the EMT process.

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CORO6 Promotes Cell Growth and Invasion of Clear Cell Renal Cell Carcinoma via Activation of WNT Signaling

Cited by 4 publications

References 26 publications

NEK2 Serves as a Novel Biomarker and Enhances the Tumorigenicity of Clear-CellRenal-Cell Carcinoma by Activating WNT/β-Catenin Pathway

NEK2 Serves as a Novel Biomarker and Enhances the Tumorigenicity of Clear-CellRenal-Cell Carcinoma by Activating WNT/β-Catenin Pathway

Highly expressed of BID indicates poor prognosis and mediates different tumor microenvironment characteristics in clear cell renal cell carcinoma

Ligustrazine Inhibits the Migration and Invasion of Renal Cell Carcinoma

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