Corona charged aerosol detector non-uniform response factors of purified alcohol ethoxylated homologues using liquid chromatography

Dufour, Alizée; Thiébaut, Didier; Loriau, Matthieu; Ligiero, Leticia; Vial, Jérôme

doi:10.1016/j.chroma.2020.461402

Cited by 2 publications

(1 citation statement)

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“…Preparative HPLC (Prep-HPLC) is a physical performance testing instrument used in the fields of biology and chemical engineering. According to the load of the sample to be separated, preparative HPLC can be divided into two types: research and development type, and industrial production type [40]. Research-type preparative HPLC belongs to laboratory-scale preparative separation, with sample sizes from micrograms to grams.…”

Section: Introductionmentioning

confidence: 99%

Purification of Two Taxanes from Taxus cuspidata by Preparative High-Performance Liquid Chromatography

Zhao

Tang

et al. 2022

Separations

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In the present study, an effective method of preparative high-performance liquid chromatography (Prep-HPLC) was established to purify two taxanes in Taxus cuspidata. During the experimental operation, the effects of flow rate, injection volume, and column temperature on the purity of 10-deacetyltaxol (10-DAT) and paclitaxel (PTX) were investigated, and the optimized conditions were as follows: flow rate of 10 mL/min, injection volume of 0.5 mL, and column temperature of 30 °C. Under these conditions, the purity of 10-DAT and PTX reached 95.33% and 99.15%, respectively. The purified products were characterized by scanning electron microscopy (SEM), high-performance liquid chromatography (HPLC), and electrospray ionization-high resolution mass spectrometry (ESI-HRMS). The results demonstrated that preparative HPLC can effectively purify 10-DAT and PTX from Taxus cuspidata with a purity of >95%, which was suitable for the large-scale preparation of 10-DAT and PTX.

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Section: Introductionmentioning

confidence: 99%