Corona discharges with water electrospray for Escherichia coli biofilm eradication on a surface

Kovaľová, Zuzana; Leroy, Magali; Kirkpatrick, Mike; Odic, Emmanuel; Machala, Zdenko

doi:10.1016/j.bioelechem.2016.05.002

Cited by 48 publications

(43 citation statements)

References 54 publications

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“…A high voltage needle electrode was inserted in a quartz tube. A DC-positive streamer corona discharge was generated on the needle electrode in ambient air, in a geometry similar to the discharge previously presented in 67,68 . The grounded electrode was a tin wire submerged in the cell culture medium at the bottom of the container.…”

Section: Methodsmentioning

confidence: 99%

Cold Atmospheric Plasma and Plasma-Activated Medium Trigger RONS-Based Tumor Cell Apoptosis

Bauer¹,

Sersenová²,

Graves³

et al. 2019

Sci Rep

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123

144

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The selective in vitro anti-tumor mechanisms of cold atmospheric plasma (CAP) and plasma-activated media (PAM) follow a sequential multi-step process. The first step involves the formation of primary singlet oxygen (1O2) through the complex interaction between NO2− and H2O2.1O2 then inactivates some membrane-associated catalase molecules on at least a few tumor cells. With some molecules of their protective catalase inactivated, these tumor cells allow locally surviving cell-derived, extracellular H2O2 and ONOO─ to form secondary 1O2. These species continue to inactivate catalase on the originally triggered cells and on adjacent cells. At the site of inactivated catalase, cell-generated H2O2 enters the cell via aquaporins, depletes glutathione and thus abrogates the cell’s protection towards lipid peroxidation. Optimal inactivation of catalase then allows efficient apoptosis induction through the HOCl signaling pathway that is finalized by lipid peroxidation. An identical CAP exposure did not result in apoptosis for nonmalignant cells. A key conclusion from these experiments is that tumor cell-generated RONS play the major role in inactivating protective catalase, depleting glutathione and establishing apoptosis-inducing RONS signaling. CAP or PAM exposure only trigger this response by initially inactivating a small percentage of protective membrane associated catalase molecules on tumor cells.

show abstract

Section: Methodsmentioning

confidence: 99%

Cold Atmospheric Plasma and Plasma-Activated Medium Trigger RONS-Based Tumor Cell Apoptosis

Bauer¹,

Sersenová²,

Graves³

et al. 2019

Sci Rep

Self Cite

123

144

View full text Add to dashboard Cite

show abstract

“…A high voltage needle electrode was inserted in a quartz tube. A DC-positive streamer corona discharge was generated on the needle electrode in ambient air, in a geometry similar to the discharge previously presented in 87,88 . The grounded electrode was a tin wire submerged in the cell culture medium at the bottom of the container.…”

Section: Methodsmentioning

confidence: 99%

Dynamics of Singlet Oxygen-Triggered, RONS-Based Apoptosis Induction after Treatment of Tumor Cells with Cold Atmospheric Plasma or Plasma-Activated Medium

Bauer

Sersenová

Graves

et al. 2019

Sci Rep

Self Cite

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Treatment of tumor cells with cold atmospheric plasma (CAP) or with plasma-activated medium (PAM) leads to a biochemical imprint on these cells. This imprint is mediated by primary singlet oxygen, which is mainly generated through the interaction between CAP-derived H2O2 and NO2−. This imprint is induced with a low efficiency as local inactivation of a few membrane-associated catalase molecules. As sustained generation of secondary singlet oxygen by the tumor cells is activated at the site of the imprint, a rapid bystander effect-like spreading of secondary singlet oxygen generation and catalase inactivation within the cell population is thus induced. This highly dynamic process is essentially driven by NOX1 and NOS of the tumor cells, and finally leads to intercellular RONS-driven apoptosis induction. This dynamic process can be studied by kinetic analysis, combined with the use of specific inhibitors at defined time intervals. Alternatively, it can be demonstrated and quantified by transfer experiments, where pretreated cells are mixed with untreated cells and bystander signaling is determined. These studies allow to conclude that the specific response of tumor cells to generate secondary singlet oxygen is the essential motor for their self-destruction, after a singlet oxygen-mediated triggering process by CAP or PAM.

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“…Nonthermal plasma treatment for bacterial decontamination has been reported for Gram-positive, Gram-negative, and biofilm-forming bacteria [ 33 , 37 , 38 ]. That bacterial inactivation was applied in the open air at atmospheric pressure or in a vacuum chamber instrument [ 39 , 40 ].…”

Section: Resultsmentioning

confidence: 99%

Effects of Atmospheric Plasma Corona Discharges on Soil Bacteria Viability

et al. 2020

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Crop contamination by soil-borne pathogenic microorganisms often leads to serious infection outbreaks. Plant protection requires disinfection of agricultural lands. The chemical and the physical disinfection procedures have several disadvantages, including an irreversible change in the soil ecosystem. Plasma, the “fourth state of matter” is defined as an ionized gas containing an equal number of negatively and positively charged particles. Cold-plasma technology with air or oxygen as the working gas generates reactive oxygen species, which are found to efficiently eradicate bacteria. In this study, we examined the effect of atmospheric plasma corona discharges on soil bacteria viability. Soil that was exposed to plasma for 60 s resulted in bacterial reduction by two orders of magnitude, from 1.1 × 105 to 2.3 × 103 cells g−1 soil. Exposure for a longer period of 5 min did not lead to further significant reduction in bacterial concentration (a final reduction of only 2.5 orders of magnitude). The bacterial viability was evaluated using a colorimetric assay based on the bacterial hydrogenases immediately after exposure and at selected times during 24 h. The result showed no recovery in the bacterial viability. Plasma discharged directly on bacteria that were isolated from the soil resulted in a reduction by four orders of magnitude in the bacterial concentration compared to untreated isolated bacteria: 2.6 × 10−3 and 1.7 × 10−7, respectively. The plasma-resistant bacteria were found to be related to the taxonomic phylum Firmicutes (98.5%) and comprised the taxonomic orders Bacillales (95%) and Clostridiales (2%). To our knowledge, this is the first study of soil bacteria eradication using plasma corona discharges.

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Corona discharges with water electrospray for Escherichia coli biofilm eradication on a surface

Cited by 48 publications

References 54 publications

Cold Atmospheric Plasma and Plasma-Activated Medium Trigger RONS-Based Tumor Cell Apoptosis

Cold Atmospheric Plasma and Plasma-Activated Medium Trigger RONS-Based Tumor Cell Apoptosis

Dynamics of Singlet Oxygen-Triggered, RONS-Based Apoptosis Induction after Treatment of Tumor Cells with Cold Atmospheric Plasma or Plasma-Activated Medium

Effects of Atmospheric Plasma Corona Discharges on Soil Bacteria Viability

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