Coronary artery bypass grafting (CABG)

Skorpil, J; Hájek, Tomáš

doi:10.1533/9780857096029.3.176

Cited by 1 publication

(1 citation statement)

References 19 publications

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“…However, there are limitations to the use of donated blood vessels in this procedure, including donor insufficiency [9] and donorsite morbidity [10]. Consequently, to overcome these issues, synthetic vascular grafts (which are categorised as acellular or cellular) have been developed and clinically utilised [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Double-layered blood vessels over 3 mm in diameter extruded by the inverse-gravity technique

Duong,

Nguyen,

Phan

et al. 2023

Biofabrication

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One of the most promising techniques for treating severe peripheral artery disease is the use of cellular tissue-engineered vascular grafts (TEVGs). This study proposes an inverse-gravity (IG) extrusion technique for creating long double-layered cellular TEVGs with diameters over 3 mm. A three-layered coaxial laminar hydrogel flow in an 8 mm-diameter pipe was realized simply by changing the extrusion direction of the hydrogel from being aligned with the direction of gravity to against it. This technique produced an extruded mixture of human aortic smooth muscle cells (HASMCs) and Type-I collagen as a tubular structure with an inner diameter of 3.5 mm. After a 21-day maturation period, the maximal burst pressure, longitudinal breaking force, and circumferential breaking force of the HASMC TEVG were 416 mmHg, 0.69 N, and 0.89 N, respectively. The HASMC TEVG was endothelialized with human umbilical vein endothelial cells to form a tunica intima that simulated human vessels. Besides subcutaneous implantability on mice, the double-layered blood vessels showed a considerably lower adherence of platelets and red blood cells once exposed to heparinized mouse blood and were considered nonhemolytic. The proposed IG extrusion technique can be applied in various fields requiring multilayered materials with large diameters.

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