Coronary leukocyte activation in relation to progression of coronary artery disease

Abstract

Leukocyte activation has been linked to atherogenesis, but there is little in vivo evidence for its role in the progression of atherosclerosis. We evaluated the predictive value for progression of coronary artery disease (CAD) of leukocyte activation markers in the coronary circulation. Monocyte and neutrophil CD11b, neutrophil CD66b expression and intracellular neutrophil myeloperoxidase (MPO) in the coronary arteries were determined by flow cytometry in patients undergoing coronary angiography. The primary outcome included fatal and nonfatal myocardial infarction or arterial vascular intervention due to unstable angina pectoris. In total 99 subjects who were included, 70 had CAD at inclusion (26 patients had single-vessel disease, 18 patients had twovessel disease and 26 patients had three-vessel disease). The median follow-up duration was 2242 days (interquartile range: 2142-2358). During follow-up, 13 patients (13%) developed progression of CAD. Monocyte CD11b, neutrophil CD11b and CD66b expression and intracellular MPO measured in blood obtained from the coronary arteries were not associated with the progression of CAD. These data indicate that coronary monocyte CD11b, neutrophil CD11b and CD66b expression and intracellular MPO do not predict the risk of progression of CAD.

Neutrophil activation and neutrophil derived neutrophil extracellular trap formation in patients with coronary artery ectasia

“…2a) between patients with obstructive coronary artery disease and individuals with normal coronary arteries were observed. These results are consistent with previous studies that demonstrated neutrophil activation may be a unique characteristic for coronary artery ectasia [20]. These results and our ROC analysis suggest that a lower MPXI (< 1.7) is an independent predictor for CAE.…”

“…Fourth, this study lacked preclinical data to support the selectins-IL-1β-neutrophil activation-NETs axis. Finally, there may be more accurate markers for neutrophil activation and NET formation, i.e., neutrophil CD11b and CD66b are direct markers for neutrophil activation, however, measurement of these markers requires rapid isolation of neutrophils [20].…”

Identification of differentially expressed genes and the role of PDK4 in CD14+ monocytes of coronary artery disease

“…In the early stage, monocytes were activated by micro-environment of blood and cytokines released by endothelial cells, subsequently transmigrating to the subendothelial tissue and swallowing lipid to differentiate to foam cells [22] . Studies found that leukocytic activation which included neutrophils, monocytes and lymphocytes was connected with the severity of CAD [23] . T cell is an important part of early 'fatty-streak' lesion, accelerating the chronic inflammatory response in progress of lesion formation [24] .…”

Biochemical Blood Parameters of Students in the Pre-Exam Period of Study

“…Встановлені зміни відображають загальну реакцію організму обстежуваних і вказують на формування окислювального стресу за участі як ліпідних, так і білкових компонентів, та пригнічення механізмів антиоксидантного захисту, спрямованих на зниження рівня активних форм кисню та продуктів вільнорадикального окиснення макромолекул. Багатьма експериментальними та клінічними дослідженнями доведено, що вільнорадикальна модифікація ліпопротеїнів низької та дуже низької густини на тлі запальної реакції та оксидативного стресу лежить в основі зростання атерогенного потенціалу крові, механізмів ініціації і прогресування атерогенного ураження різних судинних басейнів в організмі [26,27,28]. Тому постало питання визначення активності запальної реакції в організмі студентів у передекзаменаційний період, зокрема наявності запалення низької градації.…”