Coronaviridae

Tyrrell, D. A. J.; Almeida, June D.; Cunningham, Charles H.; Dowdle, Walter R.; Hofstad, M. S.; McIntosh, Kenneth; Tajima, M.; Zakstelskaya, L. Ya.; Easterday, B. C.; Kapikian, A.K.; Bingham, R. W.

doi:10.1159/000149883

Cited by 148 publications

(93 citation statements)

References 7 publications

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“…Our data on the structure and biochemistry of TGEV would support the recent proposal that coronaviruses should be classified in the monogeneric family, Coronaviridae (Tyrell et al 1975).…”

Section: Sulphation Of the Virus Glyeoproteinssupporting

confidence: 85%

Isolation of Subviral Components from Transmissible Gastroenteritis Virus

Garwes¹,

Pocock²,

Pike³

1976

Journal of General Virology

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SUMMARYExposure of purified transmissible gastroenteritis virus, a porcine coronavirus, to non-ionic detergents resulted in the removal of the surface projections and > 98 % of the virus lipid. Virus RNA was associated with a subviral particle which had a sedimentation coefficient of 65olS, compared with 495 S for the intact virion, and which banded in Cs2SO4 gradients at 1.295 g/ml. Negatively stained preparations of subviral particles were shown by electron microscopy to contain spherical particles of 6o to 7o nm diam., similar in appearance to those derived from oncornaviruses.Polyacrylamide gel electrophoresis of the polypeptides from isolated subviral particles showed that these structures contained three of the four major virus structural proteins, the arginine-rich polypeptide VP2 and the two membrane glycopolypeptides VP3 and 4. The detergent-liberated surface projections, composed of a single species of sulphated glycopolypeptide, VPr, were isolated by rate-zonal centrifugation through sucrose gradients followed by precipitation with ammonium sulphate in the presence of bovine serum albumin.

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“…Our data on the structure and biochemistry of TGEV would support the recent proposal that coronaviruses should be classified in the monogeneric family, Coronaviridae (Tyrell et al 1975).…”

Section: Sulphation Of the Virus Glyeoproteinssupporting

confidence: 85%

Isolation of Subviral Components from Transmissible Gastroenteritis Virus

Garwes¹,

Pocock²,

Pike³

1976

Journal of General Virology

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“…The polypeptide compositions of four viruses in this group have so far been described: human coronavirus strains OC43 (Hierholzer et al I972) and 229E (Hierholzer,t976); a porcine coronavirus causing transmissible gastroenteritis (TGEV; ; and the avian coronavirus, infectious bronchitis (IBV; Bingham, I975;Collins, Alexander & Harkness, 1976). Comparison of the polypeptide patterns obtained for the two human coronaviruses purified and analysed by the same techniques show marked similarities.…”

Section: Introductionmentioning

confidence: 99%

The Polypeptides of Haemagglutinating Encephalomyelitis Virus and Isolated Subviral particles

Pocock

Garwes

1977

Journal of General Virology

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SUMMARYHaemagglutinating encephalomyelitis virus (HEV), a member of the coronavirus group, was adapted for growth in adult pig thyroid cell cultures and purified by ammonium sulphate precipitation and rate zonal centrifugation through sucrose gradients. Polyacrylamide gel electrophoresis of samples of purified virus revealed the presence of five polypeptides, four of which contained carbohydrate. The molecular weights of these proteins were I8OOOO (gp I8O), I25 ooo (gp t25), IOOOOO (gp IOO), 56o00 (p 56) and 26500 (gp 26"5). After treatment of the virus with the non-ionic detergent Nonidet P4o, two subviral components were isolated. As RNA-containing particle, sedimenting in sucrose gradients at the same rate as untreated virus, was analysed and found to contain two polypeptides, p 56 and gp 26"5. The second complex sedimented at a much slower rate and contained three glycoproteins gp I8O, gp t25 and gp too. Comparison of these findings with data published for other members of the coronavirus group is discussed.

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“…Because of its spherical shape and the spikelike protrusions on the surface of the viral envelope, the name coronavims (corona is Latin for 'crown') was proposed for this group. In 1975 an official family (Coronaviridae) with a single genus (coronavirus) was designated to include MHV and IBV, among others (Tyrell et al 1975). While the relationship between the different coronaviruses is complex, the coronavirus genus has been divided into four groups based on antigenical and genetical relatedness (Pederson et al 1978).…”

mentioning

confidence: 99%

Enterotropic mouse hepatitis virus

Homberger

1997

Lab Anim

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SummaryMouse hepatitis virus (MHVI, the coronavirus of the mouse, is the most common viral pathogen in contemporary laboratory mouse colonies throughout the world. It is highly contagious with variable clinical manifestations. The majority of infections are subclinical, but can still significantly influence biological responses, thus interfering with research, mainly in the field of immunology. MHV has been intensively studied from a number of research perspectives and has become the prototype for studying the molecular biology of coronaviruses. MHV contains a single-stranded} positive-sense RNA genome ranging from 27 to 31 kb}which is divided into seven genes. Virions consist of four to five structural proteins. There are many MHV strains that vary in virulence, organotropism and cell tropism} and are constantly evolving by naturally occurring mutation and recombination. Based on pathogenesis studies MHV strains are usually grouped according to their primary tissue tropism into two biotypes: polytropic and enterotropic. Enterotropic strains of MHV replicate in the intestinal mucosa and only rarely spread to other tissues. No morphological structure of the virion has as yet been identified that is responsible for enterotropism. The course of an MHV infection is dependent on the virus strain and host factors. Generally, MHV causes an acute, self-limiting infection which is inapparent in adult mice. Neonates are highly susceptible to disease and show high mortality. In an enzootically infected colony, however, they are protected by maternally derived passive immunity. Detection of MHV infections depends on serological screening of colonies. MHV is controlled by culling and rederivation of the affected colony using hysterectomy or embryo transfer or by elimination through cessation of breeding.

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Coronaviridae

Cited by 148 publications

References 7 publications

Isolation of Subviral Components from Transmissible Gastroenteritis Virus

Isolation of Subviral Components from Transmissible Gastroenteritis Virus

The Polypeptides of Haemagglutinating Encephalomyelitis Virus and Isolated Subviral particles

Enterotropic mouse hepatitis virus

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