Coronaviruses

Monto, Arnold S.

doi:10.1007/978-1-4613-0705-1_7

Cited by 12 publications

(15 citation statements)

References 87 publications

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“…In addition, we demonstrated that this measurement system could detect SARS-CoV-2 IgM/IgG without any false-positive results by evaluating 100 serum samples collected in 2017, when SARS-CoV-2 did not exist. These results confirm that this measurement system might be able to detect antibodies against SARS-CoV-2 alone, without cross-reactivity with other coronavirus strains that cause 15%-29% of all common colds [25,26]. Chemiluminescence immunoassays are known to be affected by autoantibodies, such as rheumatoid factor, relatively often [27].…”

Section: Discussionsupporting

confidence: 72%

Validation of a new automated chemiluminescent anti-SARS-CoV-2 IgM and IgG antibody assay system detecting both N and S proteins in Japan

et al. 2021

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PCR methods are presently the standard for the diagnosis of Coronavirus disease 2019 (COVID-19), but additional methodologies are needed to complement PCR methods, which have some limitations. Here, we validated and investigated the usefulness of measuring serum antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using the iFlash3000 CLIA analyzer. We measured IgM and IgG titers against SARS-CoV-2 in sera collected from 26 PCR-positive COVID-19 patients, 53 COVID-19-suspected but PCR-negative patients, and 20 and 100 randomly selected non-COVID-19 patients who visited our hospital in 2020 and 2017, respectively. The repeatability and within-laboratory precision were obviously good in validations, following to the CLSI document EP15-A3. Linearity was also considered good between 0.6 AU/mL and 112.7 AU/mL for SARS-CoV-2 IgM and between 3.2 AU/mL and 55.3 AU/mL for SARS-CoV-2 IgG, while the linearity curves plateaued above the upper measurement range. We also confirmed that the seroconversion and no-antibody titers were over the cutoff values in all 100 serum samples collected in 2017. These results indicate that this measurement system successfully detects SARS-CoV-2 IgM/IgG. We observed four false-positive cases in the IgM assay and no false-positive cases in the IgG assay when 111 serum samples known to contain autoantibodies were evaluated. The concordance rates of the antibody test with the PCR test were 98.1% for SARS-CoV-2 IgM and 100% for IgG among PCR-negative cases and 30.8% for SARS-CoV-2 IgM and 73.1% for SARS-CoV-2 IgG among PCR-positive cases. In conclusion, the performance of this new automated method for detecting antibody against both N and S proteins of SARS-CoV-2 is sufficient for use in laboratory testing.

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Section: Discussionsupporting

confidence: 72%

Validation of a new automated chemiluminescent anti-SARS-CoV-2 IgM and IgG antibody assay system detecting both N and S proteins in Japan

et al. 2021

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“…Non-human coronaviruses like the mouse hepatitis virus in rats and haemagglutinating encephalomyelitis virus in pigs could cause demyelination and central nervous system (CNS) infections [17]. Since the new SARS associated coronavirus (SARS-CoV) was postulated to be originated from animals [9], direct involvement of nervous system in SARS was not impossible.…”

Section: Discussionmentioning

confidence: 99%

The effects of disease severity, use of corticosteroids and social factors on neuropsychiatric complaints in severe acute respiratory syndrome (SARS) patients at acute and convalescent phases

Sheng¹,

Cheng

Lau³

et al. 2005

Eur. psychiatr.

107

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“…When infected, the respiratory epithelial cells undergo ciliary damage and become vacuolated [ 46 ]. In this situation, there is the production of inflammatory mediators that promote nasal secretions, inflammation, and local swelling, as well as stimulate sneezing, obstruction of the airways, and increase of the temperature of the mucosa [ 47 ]. In this manner, SARS-CoV-2 inserts into type 2 pneumocytes, cardiomyocytes, and macrophages via ACE2 [ 23 ].…”

Section: Covid-19 and Cardiovascular Diseasementioning

confidence: 99%

Potential Therapeutic Role of Purinergic Receptors in Cardiovascular Disease Mediated by SARS-CoV-2

Anjos

Simões

Assmann

et al. 2020

Journal of Immunology Research

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Novel coronavirus disease 2019 (COVID-19) causes pulmonary and cardiovascular disorders and has become a worldwide emergency. Myocardial injury can be caused by direct or indirect damage, particularly mediated by a cytokine storm, a disordered immune response that can cause myocarditis, abnormal coagulation, arrhythmia, acute coronary syndrome, and myocardial infarction. The present review focuses on the mechanisms of this viral infection, cardiac biomarkers, consequences, and the possible therapeutic role of purinergic and adenosinergic signalling systems. In particular, we focus on the interaction of the extracellular nucleotide adenosine triphosphate (ATP) with its receptors P2X1, P2X4, P2X7, P2Y1, and P2Y2 and of adenosine (Ado) with A2A and A3 receptors, as well as their roles in host immune responses. We suggest that receptors of purinergic signalling could be ideal candidates for pharmacological targeting to protect against myocardial injury caused by a cytokine storm in COVID-19, in order to reduce systemic inflammatory damage to cells and tissues, preventing the progression of the disease by modulating the immune response and improving patient quality of life.

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Coronaviruses

Cited by 12 publications

References 87 publications

Validation of a new automated chemiluminescent anti-SARS-CoV-2 IgM and IgG antibody assay system detecting both N and S proteins in Japan

Validation of a new automated chemiluminescent anti-SARS-CoV-2 IgM and IgG antibody assay system detecting both N and S proteins in Japan

The effects of disease severity, use of corticosteroids and social factors on neuropsychiatric complaints in severe acute respiratory syndrome (SARS) patients at acute and convalescent phases

Potential Therapeutic Role of Purinergic Receptors in Cardiovascular Disease Mediated by SARS-CoV-2

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