Corosolic acid: antiangiogenic activity and safety of intravitreal injection in rats eyes

Toledo, Cibele Rodrigues; Pereira, Vinícius Oliveira de Moura; Dourado, Lays Fernanda Nunes; Paiva, Mayara Rodrigues Brandão de; Silva-Cunha, Armando

doi:10.1007/s10633-019-09682-x

Cited by 16 publications

(13 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ARPE-19 cells are present in many in vitro protocols that involve the investigation of ocular diseases [25,35]. In our study, we demonstrated that PnPa11 or PnPa13, within the range of tested concentration did not promote significant cytotoxic effects, indicating their safety for retina cells.…”

Section: Discussionmentioning

confidence: 53%

See 1 more Smart Citation

Intravitreal injection of peptides PnPa11 and PnPa13, derivatives of Phoneutria nigriventer spider venom, prevents retinal damage

Dourado

Silva

Toledo

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

Self Cite

View full text Add to dashboard Cite

Background: PnPa11 and PnPa13 are synthetic peptides derived from Phoneutria nigriventer spider venom, which display antinociceptive and neuroprotective properties. In this work, we evaluated the safety of intravitreal use and the neuroprotective effect of these peptides. Methods: The cytotoxicity and the antiangiogenic activity of these peptides were evaluated by the sulforhodamine-B method and chicken chorioallantoic membrane (CAM) assay, respectively. The in vivo safety was analyzed in Wistar rats that were intravitreally injected with different doses (0.50; 1.25; 2.50; 3.75 and 5.00 µg/mL) of these peptides (right eye, n = 6). The retinal function was assessed by electroretinography exams (ERG), intraocular pressure (IOP), and histological analyzes. In order to investigate the neuroprotective effect, Wistar rats received intravitreal injections (right eye, n = 6) of peptides at 1.25 µg/mL and then were exposed to blue LED light. In addition, the visual function and the retinal microstructure were verified. Results: Cytotoxicity analyses demonstrated that the peptides did not present any toxicity over ARPE-19 (adult retinal pigmented epithelial) cell line and the antiangiogenic study highlighted that the peptides promoted the reduction of blood vessels. The intravitreal injection did not cause major changes, neither induced any irreversible damage. In the retinal degeneration assay, the ERG records demonstrated that the prior treatment with PnPa11 and PnPa13 protected the retina from damage. Morphological analyses confirmed the ERG findings. Immunoblotting analyses revealed that PnPa11 increased Erk1/2, NR2A, and NR2B retinal expression after the light stress model, but did not cause Akt1 activation, while PnPa13 prevented Erk1/2 and Akt1 dephosphorylation. Conclusions: The intraocular administration of these peptides was well tolerated and presented protective activity against retinal degeneration, suggesting the potential use of these peptides as neuroprotectors in the ophthalmological field.

show abstract

Section: Discussionmentioning

confidence: 53%

“…The ARPE-19, adult retinal pigmented epithelial cell line, (Cellular Bank of Rio de Janeiro, Brazil) was incubated following the methodology described by Toledo et al, 2019 [ 25 ]. For cell viability, sulforhodamine B (SRB) colorimetric assay was carried out [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Intravitreal injection of peptides PnPa11 and PnPa13, derivatives of Phoneutria nigriventer spider venom, prevents retinal damage

Dourado

Silva

Toledo

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

Self Cite

View full text Add to dashboard Cite

show abstract

“…The analysis of the ERG recordings consisted in measuring the amplitude and implicit time of scotopic a- and b-waves during exposure to 0.01 (rods response) or 3.0 cd.s/m 2 (combined responses of cones and rods) as previously described. 24 – 26 …”

Section: Methodsmentioning

confidence: 99%

PnPP-19 Peptide as a Novel Drug Candidate for Topical Glaucoma Therapy Through Nitric Oxide Release

Silva

Dourado

Lima

et al. 2020

Trans. Vis. Sci. Tech.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cornea samples were fixed in Davidson solution (alcohol 95%, formaldehyde, glacial acetic acid, and distilled water) for 24 h [63].…”

Section: Histochemistrymentioning

confidence: 99%

Corneal UV Protective Effects of a Topical Antioxidant Formulation: A Pilot Study on In Vivo Rabbits

Palazzo

Vizzarri

Ondruška

et al. 2020

IJMS

View full text Add to dashboard Cite

This study aimed to evaluate the protective effect of a topical antioxidant and ultraviolet (UV) shielding action formulation containing riboflavin and D-α-tocopherol polyethylene glycol succinate (TPGS) vitamin E against corneal UV-induced damage in vivo rabbit eyes. In vivo experiments were performed using male albino rabbits, which were divided into four groups. The control group (CG) did not receive any UV irradiation; the first group (IG) was irradiated with a UV-B−UV-A lamp for 30 min; the second (G30) and third (G60) groups received UV irradiation for 30 and 60 min, respectively, and were topically treated with one drop of the antioxidant and shielding formulation every 15 min, starting one hour before irradiation, until the end of UV exposure. The cornea of the IG group showed irregular thickening, detachment of residual fragments of the Descemet membrane, stromal fluid swelling with consequent collagen fiber disorganization and disruption, and inflammation. The cornea of the G30 group showed edema, a mild thickening of the Descemet membrane without fibrillar collagen disruption and focal discoloration, or inflammation. In the G60 group, the cornea showed a more severe thickening, a more abundant fluid accumulation underneath the Descemet membrane with focal detachment, and no signs of severe tissue alterations, as were recorded in the IG group. Our results demonstrate that topical application of eye drops containing riboflavin and TPGS vitamin E counteracts UV corneal injury in exposed rabbits.

show abstract

Corosolic acid: antiangiogenic activity and safety of intravitreal injection in rats eyes

Cited by 16 publications

References 33 publications

Intravitreal injection of peptides PnPa11 and PnPa13, derivatives of Phoneutria nigriventer spider venom, prevents retinal damage

Intravitreal injection of peptides PnPa11 and PnPa13, derivatives of Phoneutria nigriventer spider venom, prevents retinal damage

PnPP-19 Peptide as a Novel Drug Candidate for Topical Glaucoma Therapy Through Nitric Oxide Release

Corneal UV Protective Effects of a Topical Antioxidant Formulation: A Pilot Study on In Vivo Rabbits

Contact Info

Product

Resources

About