CORR Insights®: Development of a Mouse Model of Ischemic Osteonecrosis

Margulies, Bryan S.

doi:10.1007/s11999-015-4237-6

Cited by 1 publication

(2 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The molecular mechanisms by which age affects bone repair following ischemic ON are unknown. It would be useful to develop an animal model of ischemic ON to address this important gap of knowledge 18 …”

Section: Introductionmentioning

confidence: 99%

“…It would be useful to develop an animal model of ischemic ON to address this important gap of knowledge. 18 Currently, there is no well-established animal model to investigate the age-related impairment of bone repair following ischemic ON. Although large animal models of ischemic ON have a higher potential for clinical translation, high costs, demanding husbandry, and lack of genetic models to investigate the molecular mechanisms limit their utility and hinder their use.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Development of a murine model of ischemic osteonecrosis to study the effects of aging on bone repair

Yamaguchi

Kamiya

Kuroyanagi

et al. 2021

Journal Orthopaedic Research

View full text Add to dashboard Cite

Age at onset is one of the most important predictors of outcome following ischemic osteonecrosis (ON). Currently, there is no well‐established animal model to study the effects of age on the repair process following ischemic ON. The purpose of this study was to further advance a murine model of ischemic ON using four age groups of mice to determine the effects of aging on revascularization and bone repair following ischemic ON. Ischemia was surgically induced in the distal femoral epiphysis of four age groups of skeletally immature and mature mice; juvenile (5 weeks), adolescent (12 weeks), adult (22 weeks), and middle age (52 weeks). Mice were euthanized at 2 days or 4 weeks post‐ischemia surgery to evaluate the extent of ON, revascularization, and bone repair. Terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling staining showed extensive cell death in the epiphysis of all four age groups at 2 days post‐ischemia surgery. At 4 weeks, the juvenile mice followed by the adolescent mice had significantly greater revascularization and repair of the necrotic marrow space, increased osteoblast and osteoclast numbers, and increased bone formation rates compared to the adult and middle‐age mice. Faster revascularization and bone healing were observed in the skeletally immature mice compared to the skeletally mature mice following ischemic ON. The findings resemble the clinical observation of aging on bone repair following ischemic ON. The mouse model may serve as a useful tool to investigate the mechanisms underlying the age‐related impairment of bone repair in adolescent and adult ON and to develop novel therapeutic strategies.

show abstract

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%