Correction: Concentration Determination of &gt;200 Proteins in Dried Blood Spots for Biomarker Discovery and Validation

Eshghi, Azad; Pistawka, Adam J.; Li, Jun; Chen, Michael; Sinclair, Nicholas J.; Hardie, Darryl B.; Elliott, Monica L.; Chen, Lei; Newman, Rachael; Mohammed, Yassene; Borchers, Christoph H.

doi:10.1074/mcp.aac120.002331

Cited by 2 publications

(2 citation statements)

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“…Two DBS discs (3 mm each) were punched using an Electron Microscopy Sciences (Hatfield, PA) Aluminum Punch Kit. Discs were added to 500 µl of 50 mM ammonium bicarbonate with 2% sodium deoxycholate (DOC) as in Eshghi et al 6 . Discs were incubated for 45 min at 37°C at 1400 RPM on an Eppendorf Thermomixer C (Hampton, NH).…”

Section: Methodsmentioning

confidence: 99%

“…Blood and plasma proteomics methods represent sensitive and high throughput techniques that can provide vast amounts of molecular data which has been used for the discovery and quantification of a myriad of human disease biomarkers 1–5 . Utilizing dried blood spots (DBS) for proteomic identification of biomarkers circumvents many of the drawbacks of biofluid collection and handling while providing a relatively cheap, stable, and minimally invasive method for collecting blood, even in a non‐clinical setting 6,7 …”

Section: Introductionmentioning

confidence: 99%

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Feasibility of detecting snake envenomation biomarkers from dried blood spots

Smith

Brandehoff

Pepin

et al. 2023

Analytical Science Advances

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Biofluid proteomics is a sensitive and high throughput technique that provides vast amounts of molecular data for biomarker discovery. More recently, dried blood spots (DBS) have gained traction as a stable, noninvasive, and relatively cheap source of proteomic data for biomarker identification in disease and injury. Snake envenomation is responsible for significant morbidity and mortality worldwide; however, much remains unknown about the systemic molecular response to envenomation and acquiring biological samples for analysis is a major hurdle. In this study, we utilized DBS acquired from a case of lethal rattlesnake envenomation to determine the feasibility of discovering biomarkers associated with human envenomation. We identified proteins that were either unique or upregulated in envenomated blood compared to non‐envenomated blood and evaluated if physiological response pathways and protein markers that correspond to the observed syndromes triggered by envenomation could be detected. We demonstrate that DBS provide useful proteomic information on the systemic processes that resulted from envenomation in this case and find evidence for a massive and systemic inflammatory cascade, combined with coagulation dysregulation, complement system activation, hypoxia response activation, and apoptosis. We also detected potential markers indicative of lethal anaphylaxis, cardiac arrest, and brain death. Ultimately, DBS proteomics has the potential to provide stable and sensitive molecular data on envenomation syndromes and response pathways, which is particularly relevant in low‐resource areas which may lack the materials for biofluid processing and storage.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Feasibility of detecting snake envenomation biomarkers from dried blood spots

Smith

Brandehoff

Pepin

et al. 2023

Analytical Science Advances

View full text Add to dashboard Cite

show abstract

Utility of an Archival Dried Blood Spot (DBS) Collection from HIV-Infected Individuals with and without Cancer in a Resource-Limited Setting

Zhang,

Bracci,

Leong

et al. 2024

IJMS

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The frequency of virus-associated cancers is growing worldwide, especially in resource-limited settings. One of the biggest challenges in cancer research among people living with HIV (PLWH) has been understanding how infection with both HIV and Kaposi sarcoma-associated herpesvirus (KSHV) promotes the pathogenesis of Kaposi sarcoma (KS), the most common cancer among PLWH worldwide and a significant public health problem in regions with high prevalence of HIV such as Sub-Saharan Africa (SSA). The AIDS and Cancer Specimen Resource (ACSR) provides samples for research, including dried blood spots (DBS) that were collected from large clinical epidemiology studies of KSHV and KS in PLWH conducted more than a decade ago in SSA. Here, we validated the quality of DNA derived from DBS samples from SSA studies and provided evidence of quantitative recovery of inflammatory cytokines using these DBS samples through comparison with paired frozen plasma. Significant differences in DNA, protein yields, and inflammatory biomarker levels were also observed between PLWH with/without KS. Establishing the fitness of DBS samples for studies of KS pathogenesis extends the number of projects that can be supported by these ACSR special collections and provides evidence that DBS collection for future KS research is a practical option in resource-limited settings.

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Correction: Concentration Determination of >200 Proteins in Dried Blood Spots for Biomarker Discovery and Validation

Cited by 2 publications

References 0 publications

Feasibility of detecting snake envenomation biomarkers from dried blood spots

Feasibility of detecting snake envenomation biomarkers from dried blood spots

Utility of an Archival Dried Blood Spot (DBS) Collection from HIV-Infected Individuals with and without Cancer in a Resource-Limited Setting

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