Correction: Detection of vapN in Rhodococcus equi isolates cultured from humans

Bryan, Laura; Alexander, Ellen Ruth; Lawhon, Sara D.; Cohen, Noah D.

doi:10.1371/journal.pone.0235719

Cited by 3 publications

(3 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, one isolate was positive to traA but negative to each of the plasmid type-specific markers vapA, vapB , and vapN . A similar situation was previously observed in a human isolate in a study performed in the United State of America ( 15 ), suggesting that there might be microvariability in some of the virulence plasmids' PCR target sequences. An alternative and more interesting possibility is that this may reflect the existence of an additional virulence plasmid type(s) yet to be characterized.…”

Section: Discussionsupporting

confidence: 82%

“…This means that all the R. equi literature prior to the discovery of the traA ( 13 ) and vapN ( 12 ) gene markers, particularly those studies reporting “avirulent/plasmidless” human isolates, must be interpreted with caution. Nevertheless, a significant proportion of R. equi human isolates (23 to 43%) actually appear to be devoid of a virulence plasmid, as judged by their negative reaction to the TRAVAP markers ( 13 , 15 ). This seems to be the case for most of the human strains analyzed in our study, in which the TRAVAP-negative genotype clearly predominated (53.8% of isolates).…”

Section: Discussionmentioning

confidence: 99%

“…The DNA extracted from each isolate (50 ng) was PCR-amplified in a reaction volume of 50 μL, using the traA -, vapA -, and vapB -specific primers of the TRAVAP typing system ( 13 ) updated with additional primers for the vapN gene marker for the novel pVAPN plasmid ( 12 ). The sequences of the primers are shown in Supplementary Table 1 , PCR reactions were performed using the modifications reported elsewhere ( 15 ). Previously, the isolates were confirmed as R. equi based on the amplification of a fragment of the choE gene encoding cholesterol oxidase ( 16 ).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Virulence Plasmids of Rhodococcus equi Isolates From Cuban Patients With AIDS

et al. 2021

View full text Add to dashboard Cite

Rhodococcus equi is an animal pathogen and zoonotic human opportunistic pathogen associated with immunosuppressive conditions. The pathogenicity of R. equi is linked to three animal host-associated virulence plasmids encoding a family of “Virulence Associated Proteins” (VAPs). Here, the PCR-based TRAVAP molecular typing system for the R. equi virulence plasmids was applied to 26 R. equi strains isolated between 2010 and 2016 at the Institute of Tropical Medicine “Pedro Kourí,” Cuba, from individuals living with HIV/AIDS. TRAVAP detects 4 gene markers, traA common to the three virulence plasmids, and vapA, vapB, and vapN specific to each of the host-associated plasmid types (equine pVAPA, porcine pVAPB, and ruminant pVAPN). Of the 26 isolates, six were positive to the vapB (porcine-type) marker, 4 (15.4%) to the vapA (equine-type) marker, and 1 (3.8%) to the vapN (ruminant-type) marker. Most of the isolates 14 (53.8%) were negative to all TRAVAP markers, suggesting they lacked a virulence plasmid. To our knowledge, this work is the first to report the molecular characterization of R. equi isolates from Cuba. Our findings provide insight into the zoonotic origin of R. equi infections in people and the potential dispensability of the virulence plasmid in immunosuppressed patients.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Virulence Plasmids of Rhodococcus equi Isolates From Cuban Patients With AIDS

et al. 2021

View full text Add to dashboard Cite

show abstract

Cellulitis-related Rhodococcus equi in a cat harboring VAPA-type plasmid pattern

Rocha¹,

Portilho²,

Júnior³

et al. 2021

Microbial Pathogenesis

View full text Add to dashboard Cite

Reinvestigation of the virulence of Rhodococcus equi isolates from patients with and without AIDS

Takaı̈

Sawada

Nakayama

et al. 2020

Lett. Appl. Microbiol.

View full text Add to dashboard Cite

Significance and Impact of the Study: Rhodococcus equi is a pathogenic cause of zoonotic diseases with a broad host range. This study demonstrated that virulence R. equi existed in human isolates before 1994 and reaffirmed that equine-associated pVAPA-positive, porcine-associated pVAPB-positive and bovine-or caprine-associated pVAPN-positive R. equi are widely spread globally. This study adds important information that domestic animals are major sources of human infection, thus adversely affecting public health.

show abstract

Correction: Detection of vapN in Rhodococcus equi isolates cultured from humans

Cited by 3 publications

References 3 publications

Virulence Plasmids of Rhodococcus equi Isolates From Cuban Patients With AIDS

Virulence Plasmids of Rhodococcus equi Isolates From Cuban Patients With AIDS

Cellulitis-related Rhodococcus equi in a cat harboring VAPA-type plasmid pattern

Reinvestigation of the virulence of Rhodococcus equi isolates from patients with and without AIDS

Contact Info

Product

Resources

About