Correction for Raymann et al., “Pathogenicity of Serratia marcescens Strains in Honey Bees”

Raymann, Kasie; Coon, Kerri L.; Shaffer, Zack; Salisbury, Stephen A.; Moran, Nancy A.

doi:10.1128/mbio.02855-18

Cited by 7 publications

(5 citation statements)

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“…This usually occurs in spring and, with lower frequency, in summer, due to poor weather conditions (Rowland et al, 2021). Antibiotics can impact composition and abundance of the honey bee microbial gut community (Raymann et al, 2017). Honey bee gut microbiota has many functions, including nutrients biosynthesis, digestion and detoxification of secondary plant compounds and other xenobiotics.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, gut microbiota induces immune responses by upregulating the expression of antimicrobial peptides, such as apidaecin (Nowak et al, 2021). Dysbiosis can decrease the honey bee life span and promote such pathogen invasions as Lotmaria passim, Nosema spp., Ascosphaera apis, and Serratia marcescens (Raymann et al, 2017). The aim of this study was to evaluate the impact of two different OTC summer protocols on honey bee colonies through the assessment of 1) toxicity on adult bees and brood, 2) residues in honey taken from the nest, 3) honey bee gut microbiota and 4) OTC-related antibiotic resistance genes.…”

Section: Introductionmentioning

confidence: 99%

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Impact of Oxytetracycline on Apis mellifera Colonies: Preliminary Results on Residues and Antibiotic Resistance

Mosca

Giannetti

Franco

et al. 2022

Journal of Apicultural Science

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We tested two summer protocols for oxytetracycline (OTC) administration on beehives to evaluate: toxicity for the bees, residues in honey and presence of genes encoding for OTC antibiotic resistance in bacteria isolated from the gut of adult bees. In July 2020, eighteen healthy colonies were divided into three groups. Two OTC treatment protocols - long and short, were devised, and one group was left untreated. We did not detect any toxicity on adult bees or brood but found OTC residues in the honey of the nest up to seven months after both treatments (210.3±221.2 µg/kg after long protocol; 216.2±238.4 µg/kg after short protocol), even in the untreated group (up to the mean 75.8±173.2 µg/kg). Antibiotic resistance genes were found, even in the untreated group, possibly due to the long exposure to antibiotics. More in-depth studies should be performed to verify how the long-term persistence of antibiotics impacted the beehive during the inactive season.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Impact of Oxytetracycline on Apis mellifera Colonies: Preliminary Results on Residues and Antibiotic Resistance

Mosca

Giannetti

Franco

et al. 2022

Journal of Apicultural Science

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“…Briefly, the hypervariable V4 region of the 16S rRNA gene was amplified using the universal primers 515F (TATGGTAATTGTGTGCCAGCMGCCGCGGTAA) and 806R (AGTCAGTCAGCCGGACTACHVGGGTWTCTAAT), modified with Illumina adaptors and index sequences. The V4 region is commonly used to analyse bacterial communities and has previously been used to study the bee microbiota (Powell et al, 2014;Raymann et al, 2017;Zheng et al, 2017). The V4 region was amplified in triplicate for each sample in a single PCR step using the following conditions: 95 °C for 15 min; 25 cycles of 95 °C for 20 s, 55 °C for 60 s, 72 °C for 60 s; and then 72 °C for 10 min.…”

Section: Sequencing the Gut Bacterial Communitymentioning

confidence: 99%

The genetic diversity of honeybee colonies predicts the gut bacterial diversity of individual colony members

Bridson

et al. 2021

Preprint

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The gut microbiota of social bees is relatively simple and dominated by a core set of taxa that have been reported consistently in individual workers from around the world. Yet, variation remains, and this has been shown to affect host health. We characterised the individual- and regional-scale variation in the honeybee (Apis mellifera) gut microbiota in the North West of England, and asked whether the microbiota was influenced by host genotype or landscape composition. We collected multiple honeybees from 64 colonies, and sequenced the V4 region of the 16S rRNA gene to characterise the mid- and hindgut bacterial communities. We characterised the genotype of each individual honeybee, and also the land cover surrounding each colony. The literature-defined core taxa consistently dominated across the region, despite the varied environments. However, there was variation in the relative abundance of core taxa, and colony membership explained a large proportion of this variation. Individuals from more genetically diverse colonies had more diverse microbiotas, but individual genetic diversity did not influence gut microbial diversity. There was a trend for colonies in more similar landscapes to have more similar microbiota, whilst bees from more urban landscapes had a slightly less diverse microbiota than those from less urban landscapes. Our study provides, to our knowledge, the first demonstration for any species that the gut bacterial communities of individuals can be influenced by the genotypes of other conspecifics in the population. This is particularly important for social organisms, such as honeybees, as colony rather than individual genetic diversity appears to drive gut microbial diversity, a factor related to colony health.

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“…An antimicrobial action is observed in bee bread's upper layer and higher values of total phenolics in the lower layer (Podrižnik & Božič, 2015). Microorganisms are not only involved in the production and preservation of food, honey and bee bread, but also important for honeybee health (Vásquez et al, 2012;Raymann, Shaffer, & Moran, 2017). To understand these benefits, such factors as microorganism composition lima et AL.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Protein Feed Fermentation Process for Supplementation of Apis Mellifera Honeybees

Lima

Parpinelli

Santos

et al. 2020

Journal of Apicultural Science

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AbstractAn alternative to natural food shortage for honeybees is the provision of supplements. The objective of this research was to develop a method with ideal conditions for the fermentation of a protein supplement for honeybees. The microorganisms used were Lactobacilli, Bifidobacteria and Saccharomyces and the inulin prebiotic. There was an increase in lactic acid and a decrease in pH over a five-day fermentation period. The amount of acid bacteria present was 108–109 CFU/g of supplement. In the first phase the temperature and the humidity had significantly affected the fermentation but the variables inulin and probiotic didn’t. In the second phase, the lower temperatures and higher humidity produced the best effects on the fermentative responses. It was concluded that an ideal environment with temperature, humidity, inulin and probiotics could be created which allowed the fermentation of the protein supplement in five days obtaining final product fermentative characteristics close to bee bread.

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Correction for Raymann et al., “Pathogenicity of Serratia marcescens Strains in Honey Bees”

Cited by 7 publications

References 0 publications

Impact of Oxytetracycline on Apis mellifera Colonies: Preliminary Results on Residues and Antibiotic Resistance

Impact of Oxytetracycline on Apis mellifera Colonies: Preliminary Results on Residues and Antibiotic Resistance

The genetic diversity of honeybee colonies predicts the gut bacterial diversity of individual colony members

Optimization of Protein Feed Fermentation Process for Supplementation of Apis Mellifera Honeybees

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