Correction: Hemocompatible glutaminase free <scp>l</scp>-asparaginase from marine Bacillus tequilensis PV9W with anticancer potential modulating p53 expression

Shakambari, Ganeshan; Birendranarayan, Anand Kumar; Lincy, Maria Joseph Angelaa; Kumar, Rai Sameer; Ahmad, Quazi Taushif; Ashokkumar, Balasubramaniem; Saravanan, M.; Mahesh, Ayyavu; Varalakshmi, Perumal

doi:10.1039/c6ra90030e

Cited by 4 publications

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“…Such a design with three values was formulated for the RSM study of the same isolate. 35 The relationship between the different component factors can be established as a linear or quadratic equation.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The chromatographic purifications were analyzed for the protein at 280 nm (BioPhotometer D30, Eppendorf, Germany). 35 The purified fractions of L-asparaginase were pooled and dialyzed with Tris-HCl (50 mM and pH 8.6) and were stored at −20 °C which were further used to identify the molecular weight of L-asparaginase by SDS-PAGE. 36 The purity of L-asparaginase was determined using the standard molecular weight markers of SDS-PAGE (GeneDireX-BLUltra prestained protein marker).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…FlowJo software was used for data analysis and to discriminate the cell cycle phases. 35 Study of p53 Expression in Purified-L-Asparaginase-Treated HeLa Cells by Flow Cytometry Analysis. Untreated HeLa cells and cells treated with L-asparaginase were subjected to primary antibody p53 mouse monoclonal IgG (Santa Cruz F2513), followed by secondary antibody, Alexa Fluor 488 goat antimouse IgG (H + L) cat no.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Agro Waste Utilization for Cost-Effective Production of l-Asparaginase by Pseudomonas plecoglossicida RS1 with Anticancer and Acrylamide Mitigation Potential

et al. 2017

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Agricultural wastes such as the peels of onion and garlic were used as a supplement along with l-asparagine for the very first time to produce increased yield of l-asparaginase by Pseudomonas plecoglossicida RS1. Statistical optimization strategies such as response surface methodology were used to generate a medium composition containing extracts of 0.9 (v/v) of garlic peel waste and 0.5% (v/v) onion peel waste along with 0.2% (w/w) l-asparagine, which yielded a twofold increase in the enzyme activity compared to the unsupplemented minimal (M-9) medium. The presence of l-asparagine content in the peel extract was confirmed by high-performance liquid chromatography. Further, l-asparaginase was purified to homogeneity, and identity was confirmed by matrix-assisted laser desorption ionization time-of-flight analysis. The application of the purified l-asparaginase as a therapeutic was studied in HeLa cells which showed a p53-mediated G2 cell cycle arrest. Moreover, the purified l-asparaginase showed effective acrylamide mitigation in vitro, at 6 IU, and its effective degradation was also demonstrated by the effect on chemotactic index of Caenorhabditis elegans and the restoration of the cognitive abilities of C. elegans which was coexposed to acrylamide and l-asparaginase compared to that exposed to acrylamide alone. Thus, l-asparaginase, with multipotent applications, was produced by effective waste utilization for economical commercial production.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Agro Waste Utilization for Cost-Effective Production of l-Asparaginase by Pseudomonas plecoglossicida RS1 with Anticancer and Acrylamide Mitigation Potential

et al. 2017

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“…with potential industrial and biotechnological applications. A group multi-copper oxidoreductase enzyme, laccases (E.C.1.10.3.2; benzenediol: oxygen-oxidoreductase) with broader substrate affinity to compounds such as phenolic, aniline, aromatic, amines, and some environmental pollutants have been used for decolorization of textile dyes, polymer synthesis, wine and beverage stabilization, detoxification of industrial effluents and biodegradation of environmental pollutants, in production of anti-cancerous and antibiotic drugs, ingredients for cosmetics, and hair coloring (21,154). Chitinolytic activity of some Bacillus spp.…”

Section: Production Of Lytic Enzymesmentioning

confidence: 99%

“…For example, farmers need to plant a certain amount of conventional plants alongside Bt plants in 'refuge' areas. Several reviews have been published on bioactive compounds from terrestrial and marine bacilli, their biosynthetic pathways and engineering plants using Bt genes (4,14,15,(19)(20)(21)(22)(23)(24). However, there is no comprehensive review published on baccili as source of agrobiotechnology.…”

Section: Introductionmentioning

confidence: 99%

Bacilli as sources of agrobiotechnology: recent advances and future directions

Dame

Rahman

Islam

2021

Green Chemistry Letters and Reviews

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The group bacilli represent the Gram-positive ubiquitous spore-forming bacteria. Their diversity, versatility and the ability of producing diverse secondary metabolites including enzymes created enormous potential for applications in agriculture, biotechnology, environment and medicine. The bacilli are considered as one of the most studied groups of bacteria providing plant growth-promotion and biocontrol of multiple diseases reflecting their vital role in enhancing plants' tolerance to biotic and abiotic stresses. Some of the Bacillus species are available commercially as phytostimulants, biopesticides, and biofertilizers. Genetically engineered plants such as maize, cotton, brinjal with endotoxins producing genes from Bacillus thuringiensis (Bt) has revolutionized agriculture. Many of these applications have been widely adopted in various fields as viable and environmentally friendly alternatives of synthetic chemical fertilizers and pesticides. A better understanding of the biology, ecology, and mechanisms of action of the beneficial strains of bacilli are needed for the development of products to support green biotechnology in agriculture and industries. This report comprehensively reviewed the applications of bacilli in agriculture and industry and discussed their potentials for the development of new products of biotechnological implications.

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Bacterial l‐asparaginases for cancer therapy: Current knowledge and future perspectives

Ghasemian

Al-Marzoqi

Al-Abodi³

et al. 2019

Journal Cellular Physiology

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L-Asparaginases hydrolyzing plasma L-asparagine and L-glutamine has attracted tremendous attention in recent years owing to remarkable anticancer properties. This enzyme is efficiently used for acute lymphoblastic leukemia (ALL) and lymphosarcoma and emerged against ALL in children, neoplasia, and some other malignancies. Cancer cells reduce the expression of L-asparaginase leading to their elimination. The L-asparaginase anticancerous application approach has made incredible breakthrough in the field of modern oncology through depletion of plasma L-asparagine to inhibit the cancer cells growth; particularly among children. High level of L-asparaginase enzyme production by Escherichia coli, Erwinia species, Streptomyces, and Bacillus subtilis species is highly desirable as bacterial alternative enzyme sources for anticancer therapy.Thermal or harsh conditions stability of those from the two latter bacterial species is considerable. Some enzymes from marine bacteria have conferred stability in adverse conditions being more advantageous in cancer therapy. Several side effects exerted by L-asparaginases such as hypersensitivity should be hindered or decreased through alternative therapies or use of immune-suppressor drugs. The L-asparaginase from Erwinia species has displayed remarkable traits in children with this regard. Noticeably, Erwinia chrysanthemi L-asparaginase exhibited negligible glutaminase activity representing a promising efficiency mitigating related side effects. Application of software such as RSM would optimize conditions for higher levels of enzyme production. Additionally, genetic recombination of the encoding gene would indisputably help improving enzyme traits. Furthermore, the possibility of anticancer combination therapy using two or more L-asparaginases from various sources is plausible in future studies to achieve better therapeutic outcomes with lower side effects. K E Y W O R D S bacterial L-asparaginase, cancer therapy, optimized production

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Correction: Hemocompatible glutaminase free l-asparaginase from marine Bacillus tequilensis PV9W with anticancer potential modulating p53 expression

Abstract: Correction for ‘Hemocompatible glutaminase free l-asparaginase from marine Bacillus tequilensis PV9W with anticancer potential modulating p53 expression’ by Ganeshan Shakambari et al., RSC Adv., 2016, 6, 25943–25951.

Cited by 4 publications

References 0 publications

Agro Waste Utilization for Cost-Effective Production of l-Asparaginase by Pseudomonas plecoglossicida RS1 with Anticancer and Acrylamide Mitigation Potential

Agro Waste Utilization for Cost-Effective Production of l-Asparaginase by Pseudomonas plecoglossicida RS1 with Anticancer and Acrylamide Mitigation Potential

Bacilli as sources of agrobiotechnology: recent advances and future directions

Bacterial l‐asparaginases for cancer therapy: Current knowledge and future perspectives

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