Correction: Liver AMP-Activated Protein Kinase Is Unnecessary for Gluconeogenesis but Protects Energy State during Nutrient Deprivation

Hasenour, Clinton M.; Wall, Martha L.; Ridley, D. Emerson; James, Freyja D.; Hughey, Curtis C.; Donahue, E. Patrick; Viollet, Benoı̂t; Foretz, Marc; Young, Jamey D.; Wasserman, David H.

doi:10.1371/journal.pone.0183601

Cited by 8 publications

(6 citation statements)

References 2 publications

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“…Following a one-hour acclimation period, an 80 μl arterial blood sample was obtained to determine natural isotopic enrichment of plasma glucose. Immediately after this sample acquisition, a stable isotope infusion protocol was initiated to allow for the quantification of endogenous glucose production and associated oxidative fluxes as previously performed (5,6,(26)(27)(28)(29)(30). Briefly, a 2 H 2 O (99.9%)-saline bolus containing [6,6-2 H 2 ]glucose (99%) was infused over 25 minutes to concurrently label the H 2 O pool and provide a [6,6-2 H 2 ]glucose prime (440 μmol•kg -1 ).…”

Section: Stable Isotope Infusionsmentioning

confidence: 99%

“…The details of the in vivo metabolic flux analysis methodology employed in these studies have been described previously (27). In summary, a reaction network was constructed using Isotopomer Network Compartmental Analysis (INCA) software (31).…”

Section: H/ 13 C Metabolic Flux Analysis 10mentioning

confidence: 99%

“…The reaction network defined the carbon and hydrogen transitions for hepatic glucose production and associated oxidative metabolism reactions. The flux through each network reaction was determined relative to citrate synthase flux (V CS ) by minimizing the sum of squared residuals between simulated and experimentally determined MIDs of the six fragment ions previously described (27,32). Flux estimates were repeated 50 times from random initial values.…”

Section: H/ 13 C Metabolic Flux Analysis 10mentioning

confidence: 99%

“…Goodness of fit was assessed by a chi-square test (p = 0.05) with 34 degrees of freedom. Confidence intervals of 95% were determined as previously described (27,32). Mouse body weights and the [6,6-2 H 2 ]glucose infusion rate were used to determine absolute values.…”

Section: H/ 13 C Metabolic Flux Analysis 10mentioning

confidence: 99%

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Exercise training adaptations in liver glycogen and glycerolipids require hepatic AMP-activated protein kinase in mice

Hughey,

Bracy,

Rome

et al. 2024

American Journal of Physiology-Endocrinology and Metabolism

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This study shows that the energy sensor and transducer, AMP-activated protein kinase (AMPK), is necessary for an exercise training-induced: 1) increase in liver glycogen that is necessary for accelerated glycogenolysis during exercise, 2) decrease in liver glycerolipids independent of tricarboxylic acid (TCA) cycle flux, and 3) decline in the desaturation and elongation of fatty acids comprising liver diacylglycerides. The mechanisms defined in these studies have implications for use of regular exercise or AMPK-activators in patients with fatty liver.

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Section: Stable Isotope Infusionsmentioning

confidence: 99%

Section: H/ 13 C Metabolic Flux Analysis 10mentioning

confidence: 99%

Section: H/ 13 C Metabolic Flux Analysis 10mentioning

confidence: 99%

Section: H/ 13 C Metabolic Flux Analysis 10mentioning

confidence: 99%

See 2 more Smart Citations

Exercise training adaptations in liver glycogen and glycerolipids require hepatic AMP-activated protein kinase in mice

Hughey,

Bracy,

Rome

et al. 2024

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

show abstract

“…Following a one-hour acclimation period, an 80 μl arterial blood sample was obtained to determine natural isotopic enrichment of plasma glucose. Immediately after this sample acquisition, a stable isotope infusion protocol was initiated to allow for the quantification of endogenous glucose production and associated oxidative fluxes as previously performed (5,6,(26)(27)(28)(29)(30) ]glucose prime. Four arterial blood samples (100 μl) were obtained 90-120 minutes following the [U-13 C]propionate bolus (time = 0-30 minutes of treadmill running) to determine arterial blood glucose using an Accu-Chek® glucometer (Roche Diagnostics, Indianapolis, IN, USA) and to perform 2 H/ 13 C metabolic flux analysis.…”

Section: Stable Isotope Infusionsmentioning

confidence: 99%

Exercise training adaptations in liver glycogen and glycerolipids require hepatic AMP-activated protein kinase in mice

Hughey,

Bracy,

Rome

et al. 2023

Preprint

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Regular exercise elicits adaptations in glucose and lipid metabolism that allow the body to meet energy demands of subsequent exercise bouts more effectively and mitigate metabolic diseases including fatty liver. Energy discharged during the acute exercise bouts that comprise exercise training may be a catalyst for liver adaptations. During acute exercise, liver glycogenolysis and gluconeogenesis are accelerated to supply glucose to working muscle. Lower liver energy state imposed by gluconeogenesis and related pathways activates AMP-activated protein kinase (AMPK), which conserves ATP partly by promoting lipid oxidation. This study tested the hypothesis that AMPK is necessary for liver glucose and lipid adaptations to training. Liver-specific AMPKα1α2 knockout (LAKO) and wild type (WT) mice completed sedentary and exercise training protocols. Liver nutrient fluxes were quantified at rest or during acute exercise following training. Liver metabolites and molecular regulators of metabolism were assessed. Training increased liver glycogen in WT mice, but not in LAKO mice. The inability to increase glycogen led to lower glycogenolysis, glucose production, and circulating glucose during acute exercise in trained LAKO mice. Deletion of AMPKα1α2 attenuated training-induced declines in liver diacylglycerides. In particular, training lowered the concentration of unsaturated and elongated fatty acids comprising diacylglycerides in WT mice, but not in LAKO mice. Training increased liver triacylglycerides and the desaturation and elongation of fatty acids in triacylglycerides of LAKO mice. These lipid responses were independent of differences in tricarboxylic acid cycle fluxes. In conclusion, AMPK is required for liver training adaptations that are critical to glucose and lipid metabolism.

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Oxidative phosphorylation K0.5ADP in vitro depends on substrate oxidative capacity: Insights from a luciferase-based assay to evaluate ADP kinetic parameters

Willis

Kuzmiak-Glancy

et al. 2021

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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Correction: Liver AMP-Activated Protein Kinase Is Unnecessary for Gluconeogenesis but Protects Energy State during Nutrient Deprivation

Cited by 8 publications

References 2 publications

Exercise training adaptations in liver glycogen and glycerolipids require hepatic AMP-activated protein kinase in mice

Exercise training adaptations in liver glycogen and glycerolipids require hepatic AMP-activated protein kinase in mice

Exercise training adaptations in liver glycogen and glycerolipids require hepatic AMP-activated protein kinase in mice

Oxidative phosphorylation K0.5ADP in vitro depends on substrate oxidative capacity: Insights from a luciferase-based assay to evaluate ADP kinetic parameters

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