Correction: Longitudinal Metagenomic Analysis of Hospital Air Identifies Clinically Relevant Microbes

King, Paula; Pham, Long; Waltz, Shannon; Sphar, Dan; Yamamoto, R. T.; Conrad, Dan; Taplitz, Randy; Torriani, Francesca J.; Forsyth, R. Allyn

doi:10.1371/journal.pone.0169376

Cited by 14 publications

(6 citation statements)

References 1 publication

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“…Initially, the general characteristics of the patients, as shown in Table 1, were consistent with the majority of data concerning other SARS-CoV-2 patients hospitalized in 2020, considering that no vaccines were viable, and drugs were administered only to alleviate the symptoms and avoid other infections 5,7,18,19 . However, the BMI of Group 5 was unexpected, as the most severe conditions were typically associated with overweight and obesity 20 .…”

Section: Discussionsupporting

confidence: 71%

The severity of COVID-19 upon hospital admission is associated with plasma omega-3 fatty acids

Fernandes,

Murai,

Fernandes

et al. 2024

Sci Rep

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Fatty acids are precursors of inflammatory oxylipins. In the context of COVID-19, an excessive production of pro-inflammatory cytokines is associated with disease severity. The objective was to investigate whether the baseline omega 3/omega 6 fatty acids ratio and the oxylipins were associated with inflammation and oxidative stress in unvaccinated patients with COVID-19, classified according to the severity of the disease during hospitalization. This Prospective population-based cohort study included 180 hospitalized patients with COVID-19. The patients were classified into five groups according to the severity of their disease. Group 1 was the least severe and Group 5 was the most severe. Three specific types of fatty acids—eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and arachidonic acid (AA)—as well as their enzymatic and non-enzymatic oxylipins were determined using chromatography coupled mass spectrometry. There was no difference in the ratio of omega-3 to omega-6 fatty acids between the groups (p = 0.276). However, the EPA/AA ratio was lower in Group 4 compared to Group 1 (p = 0.015). This finding was associated with an increase in both C-Reactive Protein (p < 0.001) and Interleukin-6 (p = 0.002). Furthermore, the concentration of F2-Isoprostanes was higher in Group 4 than in Group 1 (p = 0.009), while no significant changes were observed for other oxylipins among groups. Multivariate analysis did not present any standard of biomarkers, suggesting the high complexity of factors involved in the disease severity. Our hypothesis was confirmed in terms of EPA/AA ratio. A higher EPA/AA ratio upon hospital admission was found to be associated with lower concentration of C-Reactive Protein and Interleukin-6, leading to a better prognosis of hospitalized SARS-CoV-2 patients. Importantly, this beneficial outcome was achieved without any form of supplementation. The trial also provides important information that can be further applied to reduce the severity of infections associated with an uncontrolled synthesis of pro-inflammatory cytokines.Trial registration: https://clinicaltrials.gov/study/NCT04449718—01/06/2020. ClinicalTrials.gov Identifier: NCT04449718.

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Section: Discussionsupporting

confidence: 71%

The severity of COVID-19 upon hospital admission is associated with plasma omega-3 fatty acids

Fernandes,

Murai,

Fernandes

et al. 2024

Sci Rep

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“…Earlier studies reporting both the bacteria and fungi simultaneously in the built environments of hospitals have relied on the traditional culture-dependent methods ( Sarıca et al, 2002 ; Ekhaise et al, 2010 ; Okten and Asan, 2012 ; Sudharsanam et al, 2012 ; Park et al, 2013 ). The NGS approach for microbial community characterization has been a method of choice in the last few years owing to its fast, accurate, and precise estimation ( King et al, 2016 ; Tong et al, 2017 ; Gao et al, 2018 ; Comar et al, 2019 ; Chakrawarti et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have revealed the presence of different bacterial genera. For instance, a longitudinal metagenomic analysis of hospital air identified Stenotrophomonas as an opportunistic invader ( King et al, 2016 ). In a university health center in Delhi, Psychrobacter , Arthrobacter , and the ESKAPE ( Enterococcus faecium , Staphylococcus aureus , Klebsiella pneumoniae , Acinetobacter baumannii , Pseudomonas aeruginosa , and Enterobacter spp.)…”

Section: Discussionmentioning

confidence: 99%

Bacterial and fungal communities in indoor aerosols from two Kuwaiti hospitals

et al. 2022

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The airborne transmission of COVID-19 has drawn immense attention to bioaerosols. The topic is highly relevant in the indoor hospital environment where vulnerable patients are treated and healthcare workers are exposed to various pathogenic and non-pathogenic microbes. Knowledge of the microbial communities in such settings will enable precautionary measures to prevent any hospital-mediated outbreak and better assess occupational exposure of the healthcare workers. This study presents a baseline of the bacterial and fungal population of two major hospitals in Kuwait dealing with COVID patients, and in a non-hospital setting through targeted amplicon sequencing. The predominant bacteria of bioaerosols were Variovorax (9.44%), Parvibaculum (8.27%), Pseudonocardia (8.04%), Taonella (5.74%), Arthrospira (4.58%), Comamonas (3.84%), Methylibium (3.13%), Sphingobium (4.46%), Zoogloea (2.20%), and Sphingopyxis (2.56%). ESKAPEE pathogens, such as Pseudomonas, Acinetobacter, Staphylococcus, Enterococcus, and Escherichia, were also found in lower abundances. The fungi were represented by Wilcoxinia rehmii (64.38%), Aspergillus ruber (9.11%), Penicillium desertorum (3.89%), Leptobacillium leptobactrum (3.20%), Humicola grisea (2.99%), Ganoderma sichuanense (1.42%), Malassezia restricta (0.74%), Heterophoma sylvatica (0.49%), Fusarium proliferatum (0.46%), and Saccharomyces cerevisiae (0.23%). Some common and unique operational taxonomic units (OTUs) of bacteria and fungi were also recorded at each site; this inter-site variability shows that exhaled air can be a source of this variation. The alpha-diversity indices suggested variance in species richness and abundance in hospitals than in non-hospital sites. The community structure of bacteria varied spatially (ANOSIM r2 = 0.181–0.243; p < 0.05) between the hospital and non-hospital sites, whereas fungi were more or less homogenous. Key taxa specific to the hospitals were Defluvicoccales, fungi, Ganodermataceae, Heterophoma, and H. sylvatica compared to Actinobacteria, Leptobacillium, L. leptobacillium, and Cordycipitaceae at the non-hospital site (LefSe, FDR q ≤ 0.05). The hospital/non-hospital MD index > 1 indicated shifts in the microbial communities of indoor air in hospitals. These findings highlight the need for regular surveillance of indoor hospital environments to prevent future outbreaks.

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“…Recovering large amounts of DNA from aerosol samples is challenging, as is identifying their source given the incompleteness of public databases. However, whole genome shotgun approaches have also demonstrated usefulness in clinical [ 35 ] and agricultural environments. In a mixed crop field, wheat rust ( Puccinia striiformis f .…”

Section: How Can We Surveil For Pathogens?mentioning

confidence: 99%

Tools for pathogen genetic surveillance: Lessons from the ash dieback invasion of Europe

Peers,

Leggett,

Clark

et al. 2024

PLoS Pathog

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Correction: Longitudinal Metagenomic Analysis of Hospital Air Identifies Clinically Relevant Microbes

Cited by 14 publications

References 1 publication

The severity of COVID-19 upon hospital admission is associated with plasma omega-3 fatty acids

The severity of COVID-19 upon hospital admission is associated with plasma omega-3 fatty acids

Bacterial and fungal communities in indoor aerosols from two Kuwaiti hospitals

Tools for pathogen genetic surveillance: Lessons from the ash dieback invasion of Europe

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