Correction: Mek1 Down Regulates Rad51 Activity during Yeast Meiosis by Phosphorylation of Hed1

Callender, Tracy L.; Laureau, Raphaëlle; Wan, Lihong; Chen, Xiangyu; Sandhu, Rima; Laljee, Saif; Zhou, Sai; Suhandynata, Ray T.; Prugar, Evelyn; Gaines, William A.; Kwon, Youngho; Börner, G. Valentin; Nicolas, Alain; Neiman, Aaron M.; Hollingsworth, Nancy M.

doi:10.1371/journal.pgen.1006283

Cited by 6 publications

(3 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also monitored the activity of the downstream Mek1 effector kinase using three different readouts: Mek1 autophosphorylation (Ontoso et al 2013), Hed1 phosphorylation at T40 (Callender et al 2016), and histone H3 phosphorylation at T11 (Cavero et al 2016). As shown in Figure 4D, the dynamics of Mek1 activation paralleled that of Hop1-T318 phosphorylation (that is, Mec1 activity) and, again, was similar in both zip1 and zip1 htz1, except for a slight persistence of phospho-H3-T11 in zip1 htz1 at the latest time point.…”

Section: Dynamics Of Upstream Checkpoint Activationdeactivation Is Nomentioning

confidence: 99%

“…Previous studies suggest that the eventual checkpoint deactivation and recovery of meiotic progression in zip1 is consequence of the disappearance of the initial defects (likely unrepaired DSBs), presumably by using the sister chromatid instead of the homolog as template for DNA repair. This is based on the observation that deletion of RAD51, which fundamentally compromises sister chromatid recombination (Liu et al 2014;Callender et al 2016), leads to a permanent arrest in zip1 (Herruzo et al 2016) (Figure S7A). In this work we report that, like zip1 rad51, the zip1 htz1 double mutant also shows a tight meiotic block; however, the analysis of various checkpoint markers reveals that the cause of the arrest is different in zip1 rad51 and zip1 htz1.…”

Section: H2az Is Required For Proper Meiotic Developmentmentioning

confidence: 99%

“…Mek1 promotes interhomolog recombination bias by the direct phosphorylation of the recombination mediator Rad54 at T154 to attenuate its interaction with the strand-exchange Rad51 protein (Niu et al 2009). Also, the phosphorylation of Hed1 at Thr40 stabilizes this protein stimulating its inhibitory action on Rad51 (Callender et al 2016). Mek1 also exerts a spatial control on recombination bias by a synapsis-dependent mechanism involving Pch2 (Subramanian et al 2016).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Functional Impact of the H2A.Z Histone Variant During Meiosis in Saccharomyces cerevisiae

et al. 2018

View full text Add to dashboard Cite

Among the collection of chromatin modifications that influence its function and structure, the substitution of canonical histones by the so-called histone variants is one of the most prominent actions. Since crucial meiotic transactions are modulated by chromatin, here we investigate the functional contribution of the H2A.Z histone variant during both unperturbed meiosis and upon challenging conditions where the meiotic recombination checkpoint is triggered in budding yeast by the absence of the synaptonemal complex component Zip1 We have found that H2A.Z localizes to meiotic chromosomes in an SWR1-dependent manner. Although meiotic recombination is not substantially altered, the mutant (lacking H2A.Z) shows inefficient meiotic progression, impaired sporulation, and reduced spore viability. These phenotypes are likely accounted for by the misregulation of meiotic gene expression landscape observed in In the mutant, the absence of H2A.Z results in a tighter meiotic arrest imposed by the meiotic recombination checkpoint. We have found that Mec1-dependent Hop1-T318 phosphorylation and the ensuing Mek1 activation are not significantly altered in; however, downstream checkpoint targets, such as the meiosis I-promoting factors Ndt80, Cdc5, and Clb1, are drastically downregulated. The study of the checkpoint response in has also allowed us to reveal the existence of an additional function of the Swe1 kinase, independent of CDK inhibitory phosphorylation, which is relevant to restrain meiotic cell cycle progression. In summary, our study shows that the H2A.Z histone variant impacts various aspects of meiotic development adding further insight into the relevance of chromatin dynamics for accurate gametogenesis.

show abstract

Section: Dynamics Of Upstream Checkpoint Activationdeactivation Is Nomentioning

confidence: 99%

Section: H2az Is Required For Proper Meiotic Developmentmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Functional Impact of the H2A.Z Histone Variant During Meiosis in Saccharomyces cerevisiae

et al. 2018

View full text Add to dashboard Cite

show abstract

Crossing and zipping: molecular duties of the ZMM proteins in meiosis

2019

View full text Add to dashboard Cite

Accurate segregation of homologous chromosomes during meiosis depends on the ability of meiotic cells to promote reciprocal exchanges between parental DNA strands, known as crossovers (COs). For most organisms, including budding yeast and other fungi, mammals, nematodes and plants, the major CO pathway depends on ZMM proteins, a set of molecular actors specifically devoted to recognize and stabilize CO-specific DNA intermediates that are formed during homologous recombination. The progressive implementation of ZMM-dependent COs takes place within the context of the synaptonemal complex (SC), a proteinaceous structure that polymerizes between homologs and participates in close homolog juxtaposition during prophase I of meiosis.While SC polymerization starts from ZMM-bound sites and ZMM proteins are required for SC polymerization in budding yeast and the fungus Sordaria, other organisms differ in their requirement for ZMM in SC elongation. This review provides an overview of ZMM functions and discusses their collaborative tasks for CO formation and SC assembly, based on recent findings and on a comparison of different model organisms.

show abstract

Repair of DNA double-strand breaks in plant meiosis: role of eukaryotic RecA recombinases and their modulators

2022

View full text Add to dashboard Cite

Homologous recombination (HR) during meiosis is crucial for the DNA double-strand breaks (DSBs) repair that promotes the balanced segregation of homologous chromosomes and enhances genetic variation. In most eukaryotes, two recombinases RAD51 and DMC1 form nucleoprotein filaments on single-stranded DNA generated at DSB sites and play a central role in the meiotic DSB repair and genome stability. These nucleoprotein filaments perform homology search and DNA strand exchange to initiate repair using homologous template-directed sequences located elsewhere in the genome. Multiple factors can regulate the assembly, stability, and disassembly of RAD51 and DMC1 nucleoprotein filaments. In this review, we summarize the current understanding of the meiotic functions of RAD51 and DMC1 and the role of their positive and negative modulators. We discuss the current models and regulators of homology searches and strand exchange conserved during plant meiosis. Manipulation of these repair factors during plant meiosis also holds a great potential to accelerate plant breeding for crop improvements and productivity.

show abstract

Correction: Mek1 Down Regulates Rad51 Activity during Yeast Meiosis by Phosphorylation of Hed1

Abstract: In the Data Availability statement, the link for accessing the processed recombination analysis files in Dryad is incorrect. The correct link is http://dx.doi.org/10.5061/dryad.g6s2k.

Cited by 6 publications

References 1 publication

Functional Impact of the H2A.Z Histone Variant During Meiosis in Saccharomyces cerevisiae

Functional Impact of the H2A.Z Histone Variant During Meiosis in Saccharomyces cerevisiae

Crossing and zipping: molecular duties of the ZMM proteins in meiosis

Repair of DNA double-strand breaks in plant meiosis: role of eukaryotic RecA recombinases and their modulators

Contact Info

Product

Resources

About