Correction: MYC-containing amplicons in acute myeloid leukemia: genomic structures, evolution, and transcriptional consequences

L’Abbate, Alberto; Tolomeo, Doron; Cifola, Ingrid; Turchiano, Antonella; Augello, Bartolomeo; Squeo, Gabriella Maria; D’Addabbo, Pietro; Traversa, Debora; Daniele, Giulia; Lonoce, Angelo; Pafundi, Mariella; Carella, Massimo; Palumbo, Orazio; Dolnik, Anna; Muehlematter, Dominique; Schoumans, Jacqueline; Roy, Nadine Van; Bellis, Gianluca De; Martinelli, Giovanni; Merla, Giuseppe; Bullinger, Lars; Haferlach, Claudia; Storlazzi, Clelia Tiziana

doi:10.1038/s41375-018-0177-y

Cited by 8 publications

(4 citation statements)

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“…Afterward, it was discovered that the same amplification of the 8q24 region containing the PVT1 gene leads to the overexpression of circPVT1 and in a lesser amount of the lncRNA PVT1 in acute myeloid leukemia (AML) patients with cytogenetic abnormalities. The phenotype of 8q24 amplification in AML is known, but remains unclear how circPVT1 contributes to this genomic alteration ( 70 ). A third article confirms the oncogenic potential of circPVT1: the circularized transcript is high in head and neck squamous cell carcinoma (HNSCC), especially in patients with TP53 mutations, and has the ability to sponge miR-497-5p ( 71 ).…”

Section: Cancer and Circrnamentioning

confidence: 99%

Circular RNAs in Cancer – Lessons Learned From microRNAs

2018

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Circular RNAs (circRNA) are RNA molecules built from fragments of linear pre-messenger RNAs and other linear RNA species through a process termed “back-splicing” in which the 3′ and 5′ ends are joined together giving rise to a covalently uninterrupted loop. circRNAs are not new members of the RNA world; they were first discovered in the early 1990s. The novelty is their abundance in the mammalian cells, as recently thousands of circRNAs were discovered and annotated. The biogenesis of circRNAs is a partially characterized process, regulated by three different mechanisms: exon skipping, intron pairing, and RNA-binding proteins. On the other hand, the function of circRNAs remains largely unknown and only a handful of singular reports describe in detail the biological roles of some circular transcripts. In a very short period of time, numerous circRNAs were associated with various cancer types and were also identified in bodily fluids with the potential of being disease-specific biomarkers. In this review, we briefly describe the biogenesis and function of circRNAs and present the circular transcripts that were more than once reported in literature to be associated with cancer. Finally, we point out some of the difficulties encountered in the study of circRNAs in cancer, as we consider that taking these into account could accelerate and improve our understanding of the biologic and translational use of circRNAs in human diseases.

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Section: Cancer and Circrnamentioning

confidence: 99%

Circular RNAs in Cancer – Lessons Learned From microRNAs

2018

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“…Astonishingly, the 5′PVT1/3′CCDC26 chimera was the only fusion detected in a panel of 23 leukemia cell lines. 69 Nonetheless, chimeric transcripts involving PVT1 can also regulate the expression of yet unspecified target genes through “enhancer-like functions”. 70 Therefore, gene fusions involving lncRNAs keep or even gain functional diversity from their parental genes.…”

Section: Long Non-coding Rnas Comprise Gene Fusions With Promising Fu...mentioning

confidence: 99%

Breaking paradigms: Long non-coding RNAs forming gene fusions with potential implications in cancer

Sánchez-Marín,

Silva-Cázares,

Porras-Reyes

et al. 2024

Genes & Diseases

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“…While specific cancer cell types have been reported to harbor large circular DNAs derived from a single chromosomal region (35), the sequences of the majority of described ecDNAs are typically derived from more than 20 different genomic regions (7,36). To investigate whether our system can trigger the generation of chimeric ecDNAs, we selected target regions around CDK4 and MDM2 to produce ecDNA containing both fragments.…”

Section: Modelling Of Chimeric Ecdna With Crispr-c 20mentioning

confidence: 99%

Generative Modelling of Oncogene-carrying Extrachromosomal Circular DNA Biogenesis and Dynamics in Cells

Haskó,

Feng,

Arshadi

et al. 2024

Preprint

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Extrachromosomal circular DNAs (ecDNA) are focal gene amplifications frequently associated with cancer development and often indicating a poor prognosis. To understand the early dynamics of oncogene-carrying ecDNAs, we previously developed CRISPR-C, a tool for precise ecDNA generation by deleting specific chromosomal regions. Here, we adapted CRISPR-C to recreate tumor ecDNAs. This method also allowed us to enhance ecDNA generation efficiency by directly delivering Cas9 protein and sgRNAs as a ribonucleoprotein complex. By using the modified CRISPR-C, we successfully generated ecDNAs carrying oncogenes (EGFR, CDK4, MDM2, MYC, MYCN, FGFR2, ABCB1, and DHFR) in various human cell types. Furthermore, we demonstrated that our method could generate chimeric ecDNAs composed of target sequences from distant intra or inter-chromosomal regions. Using these generative ecDNA cell models, we studied the oncogene ecDNA expression and stability. The MDM2 expression was increased after CRISPR-C, while CDK4 was decreased indicating genomic-context dependent effect. The copy number of CRISPR-C generated CDK4 was ecDNA increased in cells after a long period of treatment with the CDK4 inhibitor palbociclib. Unlike CDK4, the CRISPR-C generated ABCB1 ecDNA was unstable in cells under normal growth conditions, but is stably retained when the cells were treated with colcemid, a recognized substrate for ABCB1. We thus provide valuable tools and an attractive platform for studying ecDNA biogenesisy and in vitro drug screening on ecDNA stability.

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Correction: MYC-containing amplicons in acute myeloid leukemia: genomic structures, evolution, and transcriptional consequences

Cited by 8 publications

References 0 publications

Circular RNAs in Cancer – Lessons Learned From microRNAs

Circular RNAs in Cancer – Lessons Learned From microRNAs

Breaking paradigms: Long non-coding RNAs forming gene fusions with potential implications in cancer

Generative Modelling of Oncogene-carrying Extrachromosomal Circular DNA Biogenesis and Dynamics in Cells

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