Correction of depth-induced spherical aberration for deep observation using two-photon excitation fluorescence microscopy with spatial light modulator

Matsumoto, Naoya; Inoue, Takashi; Matsumoto, Akiyuki; Okazaki, Satoshi

doi:10.1364/boe.6.002575

Cited by 29 publications

(20 citation statements)

References 23 publications

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“…PSFs of imaging systems are often distorted by system aberrations, which stem from misaligned optical elements, surface-shape error of the optical components, and refractive-index mismatch. [12][13][14][15] As a consequence, imaging performance inevitably degrades, making aberration correction an essential procedure in these applications.…”

Section: Introductionmentioning

confidence: 99%

Aberration correction method based on double-helix point spread function

et al. 2018

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Point spread function (PSF) engineering has met with lots of interest in various optical imaging techniques, including super-resolution microscopy, microparticle tracking, and extended depth-of-field microscopy. The intensity distributions of the modified PSFs often suffer from deteriorations caused by system aberrations, which greatly degrade the image contrast, resolution, or localization precision. We present an aberration correction method using a spiral-phase-based double-helix PSF as an aberration indicator, which is sensitive and quantitatively correlated to the spherical aberration, coma, and astigmatism. Superior to the routine iteration-based correction methods, the presented approach is iteration-free and the aberration coefficients can be directly calculated with the measured parameters, relieving the computing burden. The validity of the method is verified by both examining the intensity distribution of the conventional Gaussian PSF in three dimensions and observing muntjac skin fibroblast cells. This iteration-free correction method has a potential application in PSF engineering systems equipped with a spatial light modulator.

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Section: Introductionmentioning

confidence: 99%

Aberration correction method based on double-helix point spread function

et al. 2018

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“…Twisted nematic spatial light modulators (TNSLMs) are optoelectronic pixilated devices that can be employed to modulate amplitude, phase or polarization of light beams in space and time [1]. Such devices are based on liquid crystal displays and they provide us novel tools to manipulate orbital angular momentum (OAM) of light in the fields of communications [2 a 4], medicine [5,6], biology [7,8] and holography [9,10]. To obtain an accurate control of the amplitude of the incident wavefront on a TNSLM and improve the experimental results obtained through them, it is important a proper calibration of the modulation response of a TNSLM.…”

Section: Introductionmentioning

confidence: 99%

Modeling of an optoelectronic system for data coding using orbital angular momentum of light

Cuevas¹,

Acevedo²,

Torres-Moreno³

2018

Opt. Pura Apl.

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This paper shows the experimental and theoretical results obtained for encoding data using light beams with different values of orbital angular momentum. Light beams with orbital angular momentum were generated using holographic masks with different values of topological charge, an displayed using a transmission spatial light modulator. Furthermore, we showed the results obtained for the characterization of the spatial light modulator in the amplitude-coupled regime by using the retarder-rotor model. Key words:Orbital angular momentum of a light, data multiplexing, spatial light modulator, retarder-rotor model. RESUMEN:El presente trabajo muestra los resultados experimentales y teóricos obtenidos para la codificación de datos utilizando haces luminosos con diferentes valores de momento angular orbital. Los haces luminosos con momento angular orbital fueron generados utilizando mascaras holográficas con diferentes valores de carga topológica, y desplegadas dinámicamente empleando un modulador espacial de luz por transmisión. Además se muestran los resultados obtenidos para la caracterización del modulador espacial de luz en el régimen acoplado en intensidad usando el modelo retardadorrotor.Palabras clave: Momento angular orbital de la luz, codificación de datos, modulador especial de luz, modelo retardador rotor.

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“…3,4 Spatial light modulators have been shown to correct these aberrations and improve the image quality of biospecimens. 5,6 However, these reflective spatial light modulators require the user to change the basic design of an optical path in a conventional microscope.…”

Section: Introductionmentioning

confidence: 99%

Transmissive liquid-crystal device for correcting primary coma aberration and astigmatism in biospecimen in two-photon excitation laser scanning microscopy

et al. 2016

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Nemoto, "Transmissive liquid-crystal device for correcting primary coma aberration and astigmatism in biospecimen in two-photon excitation laser scanning microscopy," J. Abstract. All aberrations produced inside a biospecimen can degrade the quality of a three-dimensional image in two-photon excitation laser scanning microscopy. Previously, we developed a transmissive liquid-crystal device to correct spherical aberrations that improved the image quality of a fixed-mouse-brain slice treated with an optical clearing reagent. In this study, we developed a transmissive device that corrects primary coma aberration and astigmatism. The motivation for this study is that asymmetric aberration can be induced by the shape of a biospecimen and/or by a complicated refractive-index distribution in a sample; this can considerably degrade optical performance even near the sample surface. The device's performance was evaluated by observing fluorescence beads. The device was inserted between the objective lens and microscope revolver and succeeded in improving the spatial resolution and fluorescence signal of a bead image that was originally degraded by asymmetric aberration. Finally, we implemented the device for observing a fixed whole mouse brain with a sloping surface shape and complicated internal refractive-index distribution. The correction with the device improved the spatial resolution and increased the fluorescence signal by ∼2.4×. The device can provide a simple approach to acquiring higher-quality images of biospecimens.

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Correction of depth-induced spherical aberration for deep observation using two-photon excitation fluorescence microscopy with spatial light modulator

Cited by 29 publications

References 23 publications

Aberration correction method based on double-helix point spread function

Aberration correction method based on double-helix point spread function

Modeling of an optoelectronic system for data coding using orbital angular momentum of light

Transmissive liquid-crystal device for correcting primary coma aberration and astigmatism in biospecimen in two-photon excitation laser scanning microscopy

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