Correction of metabolic disturbances by functional food compositions in experimental obesity in CD-1 and agouti-yellow mice

Abstract

Background: To determine the most promising composition, the possibility of correction of metabolic disturbances was investigated with the use of functional food compositions in CD1 and agouti-yellow mice in experimental obesity.Methods: Agouti-yellow and CD1 mice were divided into 10 groups (control, 1/1, 1/9, 2/1, 2/9 in each line) according to diets. For 4 weeks, the groups ate a high-calorie diet. Starting with week 5th, composition 1 was added to the diet in groups 1/1 (60% lard + 39% standard laboratory feed + 1% composition 1 + 10% fructose solution), 1/9 (60% lard + 31% standard laboratory feed + 9% composition 1 + 10% fructose solution); composition 2 was added to the diet in groups 2/1 (60% lard + 39% standard laboratory feed + 1% composition 2 + 10% fructose solution), 2/9 (60% lard + 39% standard laboratory feed + 9% composition 2 + 10% fructose solution). On the day 56th, each animal was weighed, blood was taken for biochemical analysis (glucose, LDL, cholesterol and HDL) and organs (the skin of the anterior abdominal wall, skeletal muscle tissue in the area of the lateral surface of the thigh, liver, kidneys with perirenal tissue, pancreas, and thymus) were taken for histological examination.Results: In agouti-yellow mice, group 2/9, the greatest decrease in blood glucose level was observed compared to the control group by 34.6% (p < 0.05). Consumption of compositions in groups 1/9 and 2/9 led to a significant decrease in LDL and a significant decrease in total cholesterol level by 21.9% (p < 0.05) and 18.8% (p < 0.05) respectively compared to the control group. HDL was significantly higher in groups 1/9, 2/9 than in the control group. In CD-1 mice, group 2/9 showed the best result in glucose level (mean 5.78 mmol/l) among all groups receiving functional food compositions and the control group. The most pronounced decrease in LDL was observed in groups 1/9, 2/9 compared to the control group (p < 0.05). HDL value in group 2/9 significantly exceeded the mean value in the control group, by 54.8% (p < 0.05). Body mass of agouti-yellow mice and CD-1 mice in groups 1/1, 2/1, 1/9, and 2/9 significantly decreased compared to control groups. According to the results of histological examination of organs and tissues, functional food composition 2 turned out to be the most promising.Conclusion: According to the results of the study, the most promising composition was selected (composition 2). Feeding animals with this composition improved histological pictures of studied organs and tissues, biochemical blood parameters and effectively reduced body mass in both experimental animal lines, CD-1, and agouti-yellow mice, compared to the control groups. Keywords: CD-1 mice, agouti-yellow mice, experimental obesity, LDL, cholesterol

Bornyl-Containing Derivatives of Benzyloxyphenylpropanoic Acid as FFAR1 Agonists: In Vitro and In Vivo Studies

“…However, an earlier study in C57Bl/6 Ay mice noted the hyperglycemic effect of this compound at a single introduction, which subsequently decreased to approximately intact control levels in an OGTT conducted 2 weeks after the start of the study [ 15 ]. The absence of any hyperglycemic action of QS-528 in C57Bl/6J mice in the present experiment is probably due to less severe fatty liver dystrophy and impaired lipid metabolism, which is clearly evident in C57Bl/6 Ay mice [ 19 , 20 ]. Earlier in the same mice, the compound QS-528 was found to exhibit hepatoprotective effects [ 15 ], which were also confirmed in our experiment with biochemical (reduction of ALT) ( Figure 7 ) and histological examination of the liver (regression of fatty hepatosis) ( Figure 9 d).…”

Effects of complex phytoadaptogens in matobolic syndrome

“…Она продуцирует адипоцитокины, которые включают провоспалительные медиаторы интерлейкин-6 (IL-6) и фактор некроза опухоли α (TNF-α), ингибитор активатора плазминогена-1 (PAI-1). Данные цитокины вызывают воспалительную реакцию и повышают резистентность адипоцитов к инсулину [3]. Ожирение быстро прогрессирует с развитием метаболических нарушений; увеличивает риск развития сахарного диабета 2 типа, сердечно-сосудистых заболеваний, неалкогольной жировой болезни печени [3,4,5].…”

“…Данные цитокины вызывают воспалительную реакцию и повышают резистентность адипоцитов к инсулину [3]. Ожирение быстро прогрессирует с развитием метаболических нарушений; увеличивает риск развития сахарного диабета 2 типа, сердечно-сосудистых заболеваний, неалкогольной жировой болезни печени [3,4,5].…”