Correction of oxidative stress in rat blood with magnesium citrate upon experimental diabetes

Abstract

The investigation of the influence of different concentrations of magnesium citrate (100, 250 and 500 mg/kg body weight) on the lipid peroxidation processes and the activity of the antioxidant system in red blood cell of rats with experimental diabetes was conducted. Diabetes was caused by the single introduction of a 5% solution of alloxan monohydrate. The activity of the enzymes of antioxidant protection, catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase and the content of reduced glutathione were studied in red blood cells. The increasing levels of lipid hydroperoxides and thiobarbituric acid reactive substances against the backdrop of decreased activity of enzymes of antioxidant protection was observed in rats with experimental diabetes. Magnesium citrate, which for four weeks, together with drinking water was added to the diet of the animals, showed a positive normalizing effect. Particularly, the contents of LPO and TBARS declined, and activity of antioxidant systems of protection increased significantly. Our data shows that the magnesium citrate supplements can partly restore the antioxidant parameters and reduce oxidative stress in rats with alloxan-induced diabetes.