Correction of Pulmonary Abnormalities in Sftpd-/- Mice Requires the Collagenous Domain of Surfactant Protein D

Kingma, Paul S.; Zhang, Liqian; Ikegami, Machiko; Hartshorn, Kevan L.; McCormack, Francis X.; Whitsett, Jeffrey A.

doi:10.1074/jbc.m600651200

Cited by 50 publications

(60 citation statements)

References 46 publications

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“…Furthermore, no SP-A1 and SP-A2 expression was detected in the epithelial Clara cells and airway of the hTG lung (data not shown). These data indicate that the 3.7-kb human SP-C drives alveolar type II cell-specific expression of human SP-A1 and SP-A2, consistent with similar observations made when this promoter was used to drive expression of other genes in TG mice (4,53,56).…”

supporting

confidence: 72%

“…0.999) (n ϭ 1) or a low (i.e. 0.003) (n ϭ 2) SP-A1/total SP-A ratio or a ratio of about 0.5 (n ϭ 2) were used for preparation of surfactant large aggregates in this study (53). The ratio was used as a tool to select BAL fluids with potentially very high SP-A1 content and a very low SP-A2 and vice versa as well as select samples where both SP-A1 and SP-A2 products were readily present.…”

Section: Analysis Of Total Phospholipids Of Mousementioning

confidence: 99%

“…To generate a sufficient amount of the large aggregate fraction for the electron microscopy assay, BAL fluids from five mice were pooled for each sample studied. Large aggregates were prepared from BAL using sucrose gradient centrifugation, and the large aggregate fraction of surfactant was collected from the interface following centrifugation (53).…”

Section: Analysis Of Total Phospholipids Of Mousementioning

confidence: 99%

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Humanized SFTPA1 and SFTPA2 Transgenic Mice Reveal Functional Divergence of SP-A1 and SP-A2

Wang

Guo

DiAngelo

et al. 2010

Journal of Biological Chemistry

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supporting

confidence: 72%

Section: Analysis Of Total Phospholipids Of Mousementioning

confidence: 99%

Section: Analysis Of Total Phospholipids Of Mousementioning

confidence: 99%

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Humanized SFTPA1 and SFTPA2 Transgenic Mice Reveal Functional Divergence of SP-A1 and SP-A2

Wang

Guo

DiAngelo

et al. 2010

Journal of Biological Chemistry

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“…SP-D structure is critical to its function (39). Although the carbohydrate recognition domain mediates its interactions with pathogens, several in vivo studies in which mutant or full-length SP-D were expressed in Sftpd 2/2 mice lungs suggest that the full function of SP-D is dependent on a full-length, multimeric protein (40)(41)(42)(43)(44)(45)(46). The rhSP-D used in the present study is a full-length SP-D.…”

Section: Discussionmentioning

confidence: 99%

Surfactant Protein-D Inhibits Lung Inflammation Caused by Ventilation in Premature Newborn Lambs

Sato¹,

Whitsett²,

Scheule³

et al. 2010

Am J Respir Crit Care Med

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Rationale: Premature newborns frequently require manual ventilation for resuscitation during which lung injury occurs. Although surfactant protein (SP)-D regulates pulmonary inflammation, SP-D levels are low in the preterm lung. Commercial surfactants for treatment of respiratory distress syndrome do not contain SP-D. Objectives: To determine whether addition of recombinant human SP-D (rhSP-D) to commercial surfactant influences lung inflammation in ventilated premature newborn lambs. Methods: Prematurely delivered lambs (130 d gestation age) were resuscitated with 100% O 2 and peak inspiratory pressure 40 cm H 2 O for 20 minutes and then treated with Survanta or Survanta containing rhSP-D. Ventilation was then changed to regulate tidal volume at 8 to 9 ml/kg. At 5 hours of age lambs were killed for sample collection. Measurements and Main Results: Sequential blood gas and tidal volume were similar in lambs treated with or without rhSP-D, indicating that lung immaturity and ventilatory stress used to support premature lambs were comparable between the two groups. Ventilation caused pulmonary inflammation in lambs treated with surfactant alone. In contrast, surfactant containing rhSP-D decreased neutrophil numbers in bronchoalveolar lavage fluid and decreased neutrophil elastase activity in lung tissue. IL-8 mRNA and IL-8 protein were significantly decreased in the 1rhSP-D group lamb lungs, to 20% of those in controls. The addition of rhSP-D also rendered Survanta more resistant to plasma protein inhibition of surfactant function. Conclusions: Treatment with rhSP-D-containing surfactant inhibited lung inflammation and enhanced the resistance of surfactant to inhibition, supporting its potential usefulness for prevention of lung injury in the preterm newborn.

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“…Their calcium-dependent binding to the surface glycoconjugates of the pathogens enhances the uptake of the pathogen into the macrophages [93,94]. SP-A and SP-D are important to modulate the early stages of interaction between the host and mycobacteria upon the lung [95][96][97][98][99]. Both of them can bind to mycobacterial lipoarabinomannans but only SP-A can interact additionally with exposed glycoproteins in the cell wall of the pathogens [100][101][102].…”

Section: Collectin Familymentioning

confidence: 99%

Macrophage Infection by Mycobacteria

Saleh¹

2016

Mycobact Dis

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Mycobacterium tuberculosis is the etiological agent of tuberculosis. It is a pathogen that continues to draw international concerns particularly due to the emergence of multi-drug resistance and to the difficulties associated with the diagnosis and treatment of latent tuberculosis. A key process in the pathogenesis of this disease is the interaction between this pathogen and host macrophages. Invasion of the macrophages protects the pathogen from attack by the immune system, allows it to multiply while protected within the macrophages, and alters the immune response as it influences the profiles of the cytokine and chemokine responses. Key to the intracellular survival of the pathogen within the macrophages is the specific interaction between the pathogen and its virulence factors with the host. This review provides an a summary of pertinent literature on the topic of macrophage receptors utilized by the pathogen, its survival strategies within the macrophage, and the general profile of immune signalling upon exposure to the pathogen. The importance of specific macrophage receptors and certain components of the pathogen to the direction of the immune response are also discussed.

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Correction of Pulmonary Abnormalities in Sftpd-/- Mice Requires the Collagenous Domain of Surfactant Protein D

Abstract: Surfactant protein D (SP-D

Cited by 50 publications

References 46 publications

Humanized SFTPA1 and SFTPA2 Transgenic Mice Reveal Functional Divergence of SP-A1 and SP-A2

Humanized SFTPA1 and SFTPA2 Transgenic Mice Reveal Functional Divergence of SP-A1 and SP-A2

Surfactant Protein-D Inhibits Lung Inflammation Caused by Ventilation in Premature Newborn Lambs

Macrophage Infection by Mycobacteria

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