Correction: Phosphatidylcholine Supply to Peroxisomes of the Yeast Saccharomyces cerevisiae

Flis, Vid Vojko; Fankl, Ariane; Ramprecht, Claudia; Zellnig, Günther; Leitner, Erich; Hermetter, Albin; Daum, Günther

doi:10.1371/journal.pone.0140080

Cited by 3 publications

(6 citation statements)

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Section: Discussionsupporting

confidence: 92%

“…Previous data suggested that these contacts are involved in lipid transfer from the ER to peroxisomes to allow membrane expansion (Wu et al, 2020). This is in line with observations in S. cerevisiae indicating that the peroxisomal membrane can receive membrane lipids via non-vesicular transport (Raychaudhuri and Prinz, 2008) and that the ER is one of the lipid sources (Rosenberger et al, 2009;Flis et al, 2015). Cells of the H. polymorpha pex11, pex23 and pex24 single deletion strains still contain peroxisomes, but show reduced growth on methanol media, due to a partial defect in peroxisome function (Wu et al, 2020).…”

Section: Discussionsupporting

confidence: 86%

“…Studies in Saccharomyces cerevisiae revealed that also in this organism the peroxisomal membrane can receive membrane lipids via non-vesicular transport ( Raychaudhuri and Prinz, 2008 ). These lipids may derive from various sources including the ER, the vacuole, the mitochondrion and the Golgi apparatus ( Rosenberger et al, 2009 ; Flis et al, 2015 ). Also, in S. cerevisiae peroxisomes form contact sites with many other cellular membranes ( Shai et al, 2016 ; Shai et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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Yeast Vps13 is Crucial for Peroxisome Expansion in Cells With Reduced Peroxisome-ER Contact Sites

Yuan

Akşit

Boer

et al. 2022

Front. Cell Dev. Biol.

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In the yeast Hansenula polymorpha the peroxisomal membrane protein Pex11 and three endoplasmic reticulum localized proteins of the Pex23 family (Pex23, Pex24 and Pex32) are involved in the formation of peroxisome-ER contact sites. Previous studies suggested that these contacts are involved in non-vesicular lipid transfer and important for expansion of the peroxisomal membrane. The absence of Pex32 results in a severe peroxisomal phenotype, while cells lacking Pex11, Pex23 or Pex24 show milder defects and still are capable to form peroxisomes and grow on methanol. We performed transposon mutagenesis on H. polymorpha pex11 cells and selected mutants that lost the capacity to grow on methanol and are severely blocked in peroxisome formation. This strategy resulted in the identification of Vps13, a highly conserved contact site protein involved in bulk lipid transfer. Our data show that peroxisome formation and function is normal in cells of a vps13 single deletion strain. However, Vps13 is essential for peroxisome biogenesis in pex11. Notably, Vps13 is also required for peroxisome formation in pex23 and pex24 cells. These data suggest that Vps13 is crucial for peroxisome formation in cells with reduced peroxisome-endoplasmic reticulum contact sites and plays a redundant function in lipid transfer from the ER to peroxisomes.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

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Yeast Vps13 is Crucial for Peroxisome Expansion in Cells With Reduced Peroxisome-ER Contact Sites

Yuan

Akşit

Boer

et al. 2022

Front. Cell Dev. Biol.

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“…Yeast peroxisomes lack lipid biosynthetic enzymes; hence, expansion of the peroxisomal membrane relies on the supply of lipids from other sources. In S. cerevisiae, peroxisomal membrane lipids may originate from multiple sources, including the mitochondrion, the Golgi apparatus, the vacuole and the ER (Flis et al, 2015;Rosenberger et al, 2009). Indeed, evidence for non-vesicular lipid transport between the ER and peroxisomes in yeast has been reported previously (Raychaudhuri and Prinz, 2008).…”

Section: Discussionmentioning

confidence: 99%

Pex24 and Pex32 are required to tether peroxisomes to the ER for organelle biogenesis, positioning and segregation in yeast

Boer

Krikken

et al. 2020

Journal of Cell Science

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The yeast Hansenula polymorpha contains four members of the Pex23 family of peroxins, which characteristically contain a DysF domain. Here we show that all four H. polymorpha Pex23 family proteins localize to the endoplasmic reticulum (ER). Pex24 and Pex32, but not Pex23 and Pex29, predominantly accumulate at peroxisome–ER contacts. Upon deletion of PEX24 or PEX32 – and to a much lesser extent, of PEX23 or PEX29 – peroxisome–ER contacts are lost, concomitant with defects in peroxisomal matrix protein import, membrane growth, and organelle proliferation, positioning and segregation. These defects are suppressed by the introduction of an artificial peroxisome–ER tether, indicating that Pex24 and Pex32 contribute to tethering of peroxisomes to the ER. Accumulation of Pex32 at these contact sites is lost in cells lacking the peroxisomal membrane protein Pex11, in conjunction with disruption of the contacts. This indicates that Pex11 contributes to Pex32-dependent peroxisome–ER contact formation. The absence of Pex32 has no major effect on pre-peroxisomal vesicles that occur in pex3 atg1 deletion cells.

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“…Studies in Saccharomyces cerevisiae revealed that also in this organism the peroxisomal membrane can receive membrane lipids via non-vesicular transport . These lipids may derive from various sources including the ER, the vacuole, the mitochondrion and the Golgi apparatus (Rosenberger et al, 2009;Flis et al, 2015). Also, in S. cerevisiae peroxisomes form contact sites with many other cellular membranes (Shai et al, 2016;.…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms and Physiological Significance of Organelle Interactions and Cooperation - Volume II

2022

Frontiers Research Topics

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Correction: Phosphatidylcholine Supply to Peroxisomes of the Yeast Saccharomyces cerevisiae

Cited by 3 publications

References 0 publications

Yeast Vps13 is Crucial for Peroxisome Expansion in Cells With Reduced Peroxisome-ER Contact Sites

Yeast Vps13 is Crucial for Peroxisome Expansion in Cells With Reduced Peroxisome-ER Contact Sites

Pex24 and Pex32 are required to tether peroxisomes to the ER for organelle biogenesis, positioning and segregation in yeast

Molecular Mechanisms and Physiological Significance of Organelle Interactions and Cooperation - Volume II

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