Correction: Placental Passage of Humulone and Protopine in an Ex Vivo Human Perfusion System

Spiess, Deborah; Abegg, Vanessa Fabienne; Chauveau, Antoine; Treyer, Andrea; Reinehr, Michael; Oufir, Mouhssin; Duong, Elisa; Potterat, Olivier; Hamburger, Matthias; Simões‐Wüst, Ana Paula

doi:10.1055/a-1666-5352

Cited by 2 publications

(7 citation statements)

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“…After 24 h incubation, cells were differentiated with 5 µM FSK for another 24 h. All experiments were performed at least 3 times independently (in triplicate). Analysis of β -hCG concentrations–a marker of placenta cell differentiation–was performed using cell culture supernatants by standard ELISA (see 36 for additional references). Transparent 96-well flat-bottom microplates were used for all analyses.…”

Section: Methodsmentioning

confidence: 99%

“…The active ingredients of herbal extracts are multi-compound mixtures, many of which may be metabolized by the intestinal microbiota upon oral administration or by the liver once they have been absorbed. An interdisciplinary project is underway to fill this gap and address some of these issues, ranging from metabolizing phytochemicals by intestinal microbiota to intestinal absorption, liver metabolism, and passage across the placental barrier 36 . We performed an in vitro assessment in BeWo cells of the safety profile of medicinal plants that are used to treat mild NMDs.…”

Section: Introductionmentioning

confidence: 99%

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Medicinal Plants for the Treatment of Mental Diseases in Pregnancy: An In Vitro Safety Assessment

et al. 2021

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Pregnancy is a critical period for medical care, during which the well-being of woman and fetus must be considered. This is particularly relevant in managing non-psychotic mental disorders since treatment with central nervous system-active drugs and untreated NMDs may have negative effects. Some well-known herbal preparations (phytopharmaceuticals), including St. Johnʼs wort, California poppy, valerian, lavender, and hops, possess antidepressant, sedative, anxiolytic, or antidepressant properties and could be used to treat mental diseases such as depression, restlessness, and anxiety in pregnancy. Our goal was to assess their safety in vitro, focusing on cytotoxicity, induction of apoptosis, genotoxicity, and effects on metabolic properties and differentiation in cells widely used as a placental cell model (BeWo b30 placenta choriocarcinoma cells). The lavender essential oil was inconspicuous in all experiments and showed no detrimental effects. At low-to-high concentrations, no extract markedly affected the chosen safety parameters. At an artificially high concentration of 100 µg/mL, extracts from St. Johnʼs wort, California poppy, valerian, and hops had minimal cytotoxic effects. None of the extracts resulted in genotoxic effects or altered glucose consumption or lactate production, nor did they induce or inhibit BeWo b30 cell differentiation. This study suggests that all tested preparations from St. Johnʼs wort, California poppy, valerian, lavender, and hops, in concentrations up to 30 µg/mL, do not possess any cytotoxic or genotoxic potential and do not compromise placental cell viability, metabolic activity, and differentiation. Empirical and clinical studies during pregnancy are needed to support these in vitro data.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Medicinal Plants for the Treatment of Mental Diseases in Pregnancy: An In Vitro Safety Assessment

et al. 2021

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“…The ex vivo human placental perfusion model was adapted from the models of Schneider (Schneider, 1972) and Grafmüller (Grafmuller et al, 2013), and has been described in detail (Spiess et al, 2022a). In brief, one villous tree of a cotyledon (placenta lobule) was perfused by cannulation of the chorionic artery and corresponding vein.…”

Section: Equipment and Experimental Procedures Of Perfusionmentioning

confidence: 99%

“…A blood gas analysis (pH, pO 2 , pCO 2 , glucose, lactate; ABL800 FLEX) of fetal and maternal samples was performed to ensure viability and metabolic activity of placental tissue during perfusion. The production of the placental hormones beta-human chorionic gonadotropin (β-hCG) and leptin was monitored by standard ELISA to assess tissue functionality ex vivo [see (Spiess et al, 2022a) and references therein]. For β-hCG, flat-bottom microplates were coated with rabbit polyclonal anti-hCG antibody (Agilent Dako) at a 1:1′000 dilution and the mouse monoclonal anti-hCG (abcam; 1:5′000) served as secondary antibody.…”

Section: Sample Preparation and Quality Controlsmentioning

confidence: 99%

“…Data on transplacental passage of drugs can be obtained using the ex vivo human placental perfusion model (with term placentae) representative of the late stage of pregnancy (Myllynen and Vahakangas, 2013). It is considered to be the gold-standard among placental transfer models (Panigel et al, 1967;Malek et al, 2009;Grafmuller et al, 2013), and we have recently shown its usefulness for studying the transplacental transfer of phytochemicals (Spiess et al, 2022a). In vitro Transwell models utilising monolayers of confluent, human, nondifferentiated placental cells, on the other hand, reflect the transfer through a continuous layer of cytotrophoblasts (Bode et al, 2006;Vähäkangas and Myllynen, 2006;Prouillac and Lecoeur, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Transplacental passage of hyperforin, hypericin, and valerenic acid

et al. 2023

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Safe medications for mild mental diseases in pregnancy are needed. Phytomedicines from St. John’s wort and valerian are valid candidates, but safety data in pregnancy are lacking. The transplacental transport of hyperforin and hypericin (from St. John’s wort), and valerenic acid (from valerian) was evaluated using the ex vivo cotyledon perfusion model (4 h perfusions, term placentae) and, in part, the in vitro Transwell assay with BeWo b30 cells. Antipyrine was used for comparison in both models. U(H)PLC-MS/MS bioanalytical methods were developed to quantify the compounds. Perfusion data obtained with term placentae showed that only minor amounts of hyperforin passed into the fetal circuit, while hypericin did not cross the placental barrier and valerenic acid equilibrated between the maternal and fetal compartments. None of the investigated compounds affected metabolic, functional, and histopathological parameters of the placenta during the perfusion experiments. Data from the Transwell model suggested that valerenic acid does not cross the placental cell layer. Taken together, our data suggest that throughout the pregnancy the potential fetal exposure to hypericin and hyperforin – but not to valerenic acid – is likely to be minimal.

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Correction: Placental Passage of Humulone and Protopine in an Ex Vivo Human Perfusion System

Cited by 2 publications

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Medicinal Plants for the Treatment of Mental Diseases in Pregnancy: An In Vitro Safety Assessment

Medicinal Plants for the Treatment of Mental Diseases in Pregnancy: An In Vitro Safety Assessment

Transplacental passage of hyperforin, hypericin, and valerenic acid

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