Correction: Quantitative proteomics of strong and weak biofilm formers of Enterococcus faecalis reveals novel regulators of biofilm formation.

Suriyanarayanan, Tanujaa; Lin, Qingsong; Kwang, Lim Teck; Lee, Yew Mun; Truong, Tin; Seneviratne, Chaminda Jayampath

doi:10.1074/mcp.aac118.001077

Cited by 3 publications

(4 citation statements)

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“…The metabolically active biofilm growth mode that we observed in 3dwb has less mass and is easier to disperse than 12dwb. This is supported by Suriyanarayanan et al (2018) who compared the proteome of weak biofilm producers versus strong biofilm producers from Enterococcus faecalis [ 44 ]. They reported that strong biofilm producers showed lower metabolic activity compared to weak biofilm producers and suggested that it may be likely that the metabolically active biofilm cells (weak biofilm producer) result in less mass and promote dispersal rather than adhesion.…”

Section: Discussionmentioning

confidence: 84%

“…For instance, the upregulation of 3-phosphoshikimate 1-carboxyvinyltransferase ( aroA ) involved in the shikimate pathway shows an enhanced synthesis of aromatic amino acids in 3dwb. Some studies demonstrated that aroA production can result in glyphosphate resistance in pathogenic bacteria such as S. aureus and the plant pathogen Burkholderia glumae’s growth, virulence, and UV tolerance [ 42 , 43 , 44 ]. This suggests that a higher accumulation of glyphosphate in biofilm may show increased resistance levels to glyphosphate and virulence mechanisms, probably resulting in enhanced biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

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Proteome of Staphylococcus aureus Biofilm Changes Significantly with Aging

Rahman

Amirkhani

Chowdhury

et al. 2022

IJMS

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Staphylococcus aureus is a notorious biofilm-producing pathogen that is frequently isolated from implantable medical device infections. As biofilm ages, it becomes more tolerant to antimicrobial treatment leading to treatment failure and necessitating the costly removal of infected devices. In this study, we performed in-solution digestion followed by TMT-based high-throughput mass spectrometry and investigated what changes occur in the proteome of S. aureus biofilm grown for 3-days and 12-days in comparison with 24 h planktonic. It showed that proteins associated with biosynthetic processes, ABC transporter pathway, virulence proteins, and shikimate kinase pathway were significantly upregulated in a 3-day biofilm, while proteins associated with sugar transporter, degradation, and stress response were downregulated. Interestingly, in a 3-day biofilm, we observed numerous proteins involved in the central metabolism pathways which could lead to biofilm growth under diverse environments by providing an alternative metabolic route to utilize energy. In 12-day biofilms, proteins associated with peptidoglycan biosynthesis, sugar transporters, and stress responses were upregulated, whereas proteins associated with ABC transporters, DNA replication, and adhesion proteins were downregulated. Gene Ontology analysis revealed that more proteins are involved in metabolic processes in 3dwb compared with 12dwb. Furthermore, we observed significant variations in the formation of biofilms resulting from changes in the level of metabolic activity in the different growth modes of biofilms that could be a significant factor in S. aureus biofilm maturation and persistence. Collectively, potential marker proteins were identified and further characterized to understand their exact role in S. aureus biofilm development, which may shed light on possible new therapeutic regimes in the treatment of biofilm-related implant-associated infections.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Proteome of Staphylococcus aureus Biofilm Changes Significantly with Aging

Rahman

Amirkhani

Chowdhury

et al. 2022

IJMS

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“…60 The detection of common responses of bacterial strains to a biofilm growth environment has been a focus of previous research. [61][62][63][64] Uncovering core regulatory pathways that drive biofilm-associated tolerance towards environmental stressors promise to give clues to host and environmental interactions and could provide useful targets for new clinical interventions.…”

Section: Discussionmentioning

confidence: 99%

Parallel evolutionary paths to produce more than one Pseudomonas aeruginosa biofilm phenotype

Thöming

Tomasch

Preuße

et al. 2020

npj Biofilms Microbiomes

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Studying parallel evolution of similar traits in independent within-species lineages provides an opportunity to address evolutionary predictability of molecular changes underlying adaptation. In this study, we monitored biofilm forming capabilities, motility, and virulence phenotypes of a plethora of phylogenetically diverse clinical isolates of the opportunistic pathogen Pseudomonas aeruginosa. We also recorded biofilm-specific and planktonic transcriptional responses. We found that P. aeruginosa isolates could be stratified based on the production of distinct organismal traits. Three major biofilm phenotypes, which shared motility and virulence phenotypes, were produced repeatedly in several isolates, indicating that the phenotypes evolved via parallel or convergent evolution. Of note, while we found a restricted general response to the biofilm environment, the individual groups of biofilm phenotypes reproduced biofilm transcriptional profiles that included the expression of well-known biofilm features, such as surface adhesive structures and extracellular matrix components. Our results provide insights into distinct ways to make a biofilm and indicate that genetic adaptations can modulate multiple pathways for biofilm development that are followed by several independent clinical isolates. Uncovering core regulatory pathways that drive biofilm-associated growth and tolerance towards environmental stressors promises to give clues to host and environmental interactions and could provide useful targets for new clinical interventions.

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“…The present study showed MecA abundance increased in the ΔclpP mutant strain, and this contribute to the decreased biofilm formation of the clpP deleted strain. Another reason for decreased biofilm formation of the ΔclpP mutant strain may be the reduced abundances of orotate phosphoribosyltransferase (pyrE) and orotidine-5′-phosphate decarboxylase (pyrF), proteins that promote the biofilm formation of Streptococcus sanguinis and E. faecalis, respectively [38,39].…”

Section: Discussionmentioning

confidence: 99%

ClpP participates in stress tolerance, biofilm formation, antimicrobial tolerance, and virulence of Enterococcus faecalis

Zheng

Wang

et al. 2020

BMC Microbiol

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Background: ClpP is important for bacterial growth and plays an indispensable role in cellular protein quality control systems by refolding or degrading damaged proteins, but the physiological significance of ClpP in Enterococcus faecalis remains obscure. A clpP deletion mutant (△clpP) was constructed using the E. faecalis OG1RF strain to clarify the effect of ClpP on E. faecalis. The global abundance of proteins was determined by a mass spectrometer with tandem mass tag labeling. Results: The ΔclpP mutant strain showed impaired growth at 20°C or 45°C at 5% NaCl or 2 mM H 2 O 2 . The number of surviving ΔclpP mutants decreased after exposure to the high concentration (50× minimal inhibitory concentration) of linezolid or minocycline for 96 h. The ΔclpP mutant strain also demonstrated decreased biofilm formation but increased virulence in a Galleria mellonella model. The mass spectrometry proteomics data indicated that the abundances of 135 proteins changed (111 increased, 24 decreased) in the ΔclpP mutant strain. Among those, the abundances of stress response or virulence relating proteins: FsrA response regulator, gelatinase GelE, regulatory protein Spx (spxA), heat-inducible transcription repressor HrcA, transcriptional regulator CtsR, ATPase/ chaperone ClpC, acetyl esterase/lipase, and chaperonin GroEL increased in the ΔclpP mutant strain; however, the abundances of ribosomal protein L4/L1 family protein (rplD), ribosomal protein L7/L12 (rplL2), 50S ribosomal protein L13 (rplM), L18 (rplR), L20 (rplT), 30S ribosomal protein S14 (rpsN2) and S18 (rpsR) all decreased. The abundances of biofilm formation-related adapter protein MecA increased, while the abundances of dihydroorotase (pyrC), orotate phosphoribosyltransferase (pyrE), and orotidine-5′-phosphate decarboxylase (pyrF) all decreased in the ΔclpP mutant strain. Conclusion:The present study demonstrates that ClpP participates in stress tolerance, biofilm formation, antimicrobial tolerance, and virulence of E. faecalis.

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Correction: Quantitative proteomics of strong and weak biofilm formers of Enterococcus faecalis reveals novel regulators of biofilm formation.

Cited by 3 publications

References 0 publications

Proteome of Staphylococcus aureus Biofilm Changes Significantly with Aging

Proteome of Staphylococcus aureus Biofilm Changes Significantly with Aging

Parallel evolutionary paths to produce more than one Pseudomonas aeruginosa biofilm phenotype

ClpP participates in stress tolerance, biofilm formation, antimicrobial tolerance, and virulence of Enterococcus faecalis

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