Correction: Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing

Takeoka, Yosuke; Matsumoto, Keitaro; Taniguchi, Daisuke; Tsuchiya, Tomoshi; Machino, Ryusuke; Moriyama, Masaaki; Oyama, Shosaburo; Tetsuo, Tomoyuki; Taura, Yasuho; Takagi, Katsunori; Yoshida, Takuya; Elgalad, Abdelmotagaly; Matsuo, Naoto; Kitajima, Masaki; Tobinaga, Shuichi; Nonaka, Takashi; Hidaka, Shigekazu; Yamasaki, Naoya; Nakayama, Koichi; Nagayasu, Takeshi

doi:10.1371/journal.pone.0216174

Cited by 7 publications

(1 citation statement)

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“…On these bases, cells were encapsulated in ECM and fused to generate an operable macrostructure, which could be utilized to mimic the desired shape, complex morphology, and physiological tasks of natural tissues. [29][30][31][32] Herein, auricular chondrocytes were densely stacked to induce self-assembly and form operable cartilage sheets (Cart-S), in which chondrocytes could grow in a 3D pattern to promote intercellular communication and maintain phenotypic stability. The Cart-S was further laminated and reshaped to construct a cartilage tube, and the feasibility of the cartilage tube to serve as the main load-bearing structure was explored along with the morphology and differentiation of the inner chondrocytes.…”

Section: Introductionmentioning

confidence: 99%

Scaffold‐Free Tracheal Engineering via a Modular Strategy Based on Cartilage and Epithelium Sheets

Yang

Chen

et al. 2022

Adv Healthcare Materials

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Tracheal defects lead to devastating problems, and practical clinical substitutes that have complex functional structures and can avoid adverse influences from exogenous bioscaffolds are lacking. Herein, a modular strategy for scaffold‐free tracheal engineering is developed. A cartilage sheet (Cart‐S) prepared by high‐density culture is laminated and reshaped to construct a cartilage tube as the main load‐bearing structure in which the chondrocytes exhibit a stable phenotype and secreted considerable cartilage‐specific matrix, presenting a native‐like grid arrangement. To further build a tracheal epithelial barrier, a temperature‐sensitive technique is used to construct the monolayer epithelium sheet (Epi‐S), in which the airway epithelial cells present integrated tight junctions, good transepithelial electrical resistance, and favorable ciliary differentiation capability. Epi‐S can be integrally transferred to inner wall of cartilage tube, forming a scaffold‐free complex tracheal substitute (SC‐trachea). Interestingly, when Epi‐S is attached to the cartilage surface, epithelium‐specific gene expression is significantly enhanced. SC‐trachea establishes abundant blood supply via heterotopic vascularization and then is pedicle transplanted for tracheal reconstruction, achieving 83.3% survival outcomes in rabbit models. Notably, the scaffold‐free engineered trachea simultaneously satisfies sufficient mechanical properties and barrier function due to its matrix‐rich cartilage structure and well‐differentiated ciliated epithelium, demonstrating great clinical potential for long‐segmental tracheal reconstruction.

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Section: Introductionmentioning

confidence: 99%