Correction to: A role for tetraspanin proteins in regulating fusion induced by Burkholderia thailandensis

Elgawidi, Atiga; Mohsin, Muslim Idan; Ali, Fawwaz; Watts, Amyleigh; Monk, Peter N.; Thomas, Mark S.; Partridge, Lynda J.

doi:10.1007/s00430-020-00682-2

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“…There was no effect of CD9 or CD81 reagents on J774A.1 cells internalisation which might be expected to occur via phagocytosis but we observed the effect in A549 cells, where the mechanism presumably involves the T3SS. This suggests that the tetraspanins were not affecting phagocytosis 34 but may be affecting elements of the T3SS-mediated entry. CD81 has been shown to be necessary for internalisation of L. monocytogenes in which the mechanism involving phosphatidylinositol 4-kinase II 28 .…”

Section: Discussionmentioning

confidence: 95%

Tetraspanins are involved in Burkholderia pseudomallei-induced cell-to-cell fusion of phagocytic and non-phagocytic cells

Sangsri

Saiprom

Tubsuwan

et al. 2020

Sci Rep

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Tetraspanins are four-span transmembrane proteins of host cells that facilitate infections by many pathogens. Burkholderia pseudomallei is an intracellular bacterium and the causative agent of melioidosis, a severe disease in tropical regions. This study investigated the role of tetraspanins in B. pseudomallei infection. We used flow cytometry to determine tetraspanins CD9, CD63, and CD81 expression on A549 and J774A.1 cells. Their roles in B. pseudomallei infection were investigated in vitro using monoclonal antibodies (MAbs) and recombinant large extracellular loop (EC2) proteins to pretreat cells before infection. Knockout of CD9 and CD81 in cells was performed using CRISPR Cas9 to confirm the role of tetraspanins. Pretreatment of A549 cells with MAb against CD9 and CD9-EC2 significantly enhanced B. pseudomallei internalization, but MAb against CD81 and CD81-EC2 inhibited MNGC formation. Reduction of MNGC formation was consistently observed in J774.A1 cells pretreated with MAbs specific to CD9 and CD81 and with CD9-EC2 and CD81-EC2. Data from knockout experiments confirmed that CD9 enhanced bacterial internalization and that CD81 inhibited MNGC formation. Our data indicate that tetraspanins are host cellular factors that mediated internalization and membrane fusion during B. pseudomallei infection. Tetraspanins may be the potential therapeutic targets for melioidosis.

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Section: Discussionmentioning

confidence: 95%

Tetraspanins are involved in Burkholderia pseudomallei-induced cell-to-cell fusion of phagocytic and non-phagocytic cells

Sangsri

Saiprom

Tubsuwan

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

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Correction to: A role for tetraspanin proteins in regulating fusion induced by Burkholderia thailandensis

Abstract: In the original article, incorrect Figures were published with incorrect captions. The correct Figs. 1, 2, 3, 4, 5, 6, 7 and 8 and captions are given below.

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Tetraspanins are involved in Burkholderia pseudomallei-induced cell-to-cell fusion of phagocytic and non-phagocytic cells

Tetraspanins are involved in Burkholderia pseudomallei-induced cell-to-cell fusion of phagocytic and non-phagocytic cells

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