Correction to: Antioxidant and antibacterial activities of Artemisia absinthium and Citrus paradisi extracts repress viability of aggressive liver cancer cell line

Ali, Muhammad; Iqbal, Rida; Safdar, Muhammad; Murtaza, Sehrish; Mustafa, Ghulam; Sajjad, Muhammad; Bukhari, Shazia Anwer; Huma, Tayyaba

doi:10.1007/s11033-021-07103-4

Cited by 3 publications

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“…absinthium L. belongs to Campanulales, family Asteraceae, and is commonly known as Wormwood (Moacă et al, 2019). The medicinal perennial mainly distributes in Xinjiang, Sichuan, and Yunnan in China (Mohammadian et al, 2016), which is rich in secondary metabolites including avonoids, alkaloids, phenols, terpenoids, and derivatives with various pharmacological activities, such as antibacterial, anti-in ammatory, antiviral, hepatoprotective, antioxidant and anticancer (Ahmad et al, 1992;Ali et al, 2021;Juteau et al, 2003;Koyuncu, 2018;Mishra et al, 2013;Szopa et al, 2020). In addition to its traditional medical applications, it has a high food value.…”

Section: Introductionmentioning

confidence: 99%

Total flavonoids in Artemisia absinthium L. and evaluation of its anticancer activity

He,

Yasin,

et al. 2023

Preprint

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Aim of the study: To optimize the extracting parameters of total flavonoids in Artemisia absinthium L. by ultrasound-assist combined with enzymatic hydrolysis and to combine molecular docking and network pharmacology to predict its core constituent targets and thus evaluate its antitumor activity. Methods Reaction surface methodology was used to investigate the univariate effects of enzyme ratio, enzyme amount, enzyme hydrolysis temperature, enzyme hydrolysis time, pH, solid-liquid ratio, ethanol concentration, and sonication temperature on total flavonoid yield in A. absinthium L.. On this basis, a three-factor, three-level experimental approach was adopted with solid-liquid ratio, enzymatic hydrolysis temperature, and ethanol concentration as independent variables and total flavonoids as response values, and then combined with Box-Behnken design (BBD) to optimize the extraction conditions. The quantitative and qualitative analysis of the main components was performed by UHPLC-MS. The inhibitory effect of flavonoids and their active components on the proliferation of cervical cancer HeLa cells was detected by MTT. Molecular docking and network pharmacology were used to predict the molecular mechanism of the main active components (Cynaroside and Astragalin) and to decipher the overall mechanism of total flavonoids against cervical cancer.. pharmMapper and SwissTargetPrediction databases were used to sort out the potential targets of the main chemical components. Targets related to cervical cancer were collected from OMIM and DrugBank. Results The content of total flavonoids in A. absinthium L. reached 3.80 ± 0.13%, and the main components included Astragalin, Cynaroside, Ononin, Rutin, Kaempferol-3-O-rutinoside, Diosmetin, Isorhamnetin, and Luteolin. Cynaroside and Astragalin exert their cervical cancer inhibitory functions by regulating several signaling proteins (e.g. EGFR, STAT3, CCND1, IGFIR, ESR1). GO and KEGG analyses showed that the anti-cancer of both compounds was associated with ErbB signaling pathway and FoxO signaling pathway. MTT results showed that total flavonoids of A. absinthium L. and its active components (Cynaroside and Astragalin) significantly inhibited the growth of HeLa cells in a concentration-dependent manner with IC50 of 396.0 ± 54.2 µg/mL and 449.0 ± 54.8 µg/mL, respectively. Conclusion The optimal process conditions for obtaining total flavonoids by ultrasound-assisted enzymatic digestion were: enzyme ratio 3:2, enzyme dosage 2%, enzymatic hydrolysis temperature 45℃, enzymatic hydrolysis time 105 min, pH 3.5, solid-liquid ratio 1:15, ethanol concentration 85%, sonication temperature 30℃. Results from network pharmacology and molecular docking indicate that EGFR and SRC are the key targets of the two core components of total flavonoids against cervical cancer, the optimal procedure for extracting total flavonoids from A. absinthium L. was fixed and the total flavonoids showed good anti-cervical cancer activity. Furthermore, its active components can mediate apoptosis by inducing the accumulation of ROS.

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Section: Introductionmentioning

confidence: 99%

Total flavonoids in Artemisia absinthium L. and evaluation of its anticancer activity

He,

Yasin,

et al. 2023

Preprint

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Prospects of Hepatoprotective Effect of Sesquiterpene Lactone Absintin

Efremov,

Shamitova,

Romanova

2023

Problems Bio Med Pharm Chem

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Liver and gallbladder diseases are among the most common in the world today. Liver disorders occupy one of the most important places in the pic-ture of general human pathology, having a fairly high risk of developing adverse complications. Preparations and biologically active additives based on Artemisia absinthium and the components extracted from it have recently become very popular. The group of sesquiterpene lactones is of the greatest interest to researchers. The object of this review article is to analyze domestic and foreign publications showing the pharmacological activity and pro-spects of using absinthin, which is part of the group of sesquiterpene lactones of A. absinthium, as a hepatoprotector. An analysis of the literature re-vealed that absinthin has an antitoxic effect, reduces lipid peroxidation in liver tissues and restores the activity of protective antioxidant enzymes su-peroxide dismutase (SOD) and glutathione peroxidase (GPx) to a normal level. The generalization of literature data showing the pharmacological effect of the substance indicates the ability of absinthin to reduce the level of toxic effects of fatty acids and cholesterol on hepatocytes. It was also revealed that absinthin is able to have an antiproliferative effect on human adenocarcinoma cells. The positive effect observed when using absinthin character-izes the prospects of its practical application as a drug or biologically active additive with hepatoprotective and antitumor effect.

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Antitumor Activity of Species of the Genus <i>Artemisia</i> L.: Current State and Prospects (Review)

Romanteeva

Berezutsky

Kurchatova

2023

Razrabotka i registraciâ lekarstvennyh sredstv

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Introduction. Recently, the anticancer activity of representatives of the genus Artemisia L. has been actively studied, and most studies are devoted to Artemisia annua L., which has been used since ancient times in the folk medicine of several countries as an antimalarial and anticancer agent. The similarity of the chemical composition predetermines the study of the anticancer activity of other species of the genus Artemisia L. The information about this is still not fully presented in scientific publications, is very diverse and sometimes even contradictory. Review of modern studies of anticancer activity of species of the genus Artemisia L., generalization of available data and providing information for future research is relevant.Text. The article presents a review of experimental data on the study of anticancer activity of representatives of the genus Artemisia L. It is noted that the main mechanism of such activity is apoptosis. Apoptosis is triggered by the increase of reactive oxygen species (ROS) inside cancer cells, reduction of mitochondrial membrane potential, activation of pro-apoptotic and, on the contrary, inhibition of anti-apoptotic proteins, as well as by formation of membrane bubbles, cell compression and by activation caspase.Conclusion. In the presented review, about 30 species of the genus Artemisia L. With the presented degree of study of this area, a number of questions remain unresolved. The most studied with respect to cytotoxic activity are Artemisia absinthium L. and Artemisia vulgaris L. In this aspect, the study of other closely related species of the genus Artemisia L. Also relevant is the study of cytotoxicity of representatives of the genus Artemisia L. on normal cell cultures and in comparison with positive control. In addition, a detailed study of the pool of secondary metabolites of different species of the genus Artemisia L. remains significant in order to reliably determine the components responsible for the manifestation of anticancer action. The pronounced effectiveness against cancer cells and, at the same time, a weak effect on healthy cells of the body of representatives of the genus Artemisia L. opens up the prospect of their use as sources of partner drugs with a synergistic effect and means of augmentation of antitumor therapy.

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Correction to: Antioxidant and antibacterial activities of Artemisia absinthium and Citrus paradisi extracts repress viability of aggressive liver cancer cell line

Cited by 3 publications

References 0 publications

Total flavonoids in Artemisia absinthium L. and evaluation of its anticancer activity

Total flavonoids in Artemisia absinthium L. and evaluation of its anticancer activity

Prospects of Hepatoprotective Effect of Sesquiterpene Lactone Absintin

Antitumor Activity of Species of the Genus <i>Artemisia</i> L.: Current State and Prospects (Review)

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