Bone tissue is a calcium deposit and supporting structure of the human body, it is exposed to several pathologies that modify its mineral content. To determine these changes, different diagnostic procedures are performed with techniques using invasive ionizing radiation, which are limited by the negative effects in the long term on human health. A methodology is explored that could be applicable in the diagnosis of pathologic variations in bone mineral density, using structural monitoring tools. The proposed technique estimates changes in bone conditions by applying impedance spectroscopy with a tooth-borne piezo-device. Bone-tooth samples were prepared to simulate a section of maxillary bone and subsequently treated with chemical agents, simulating pathologic decalcification. The piezo-device is inserted in the slot of an orthodontic bracket, previously bonded to the crown of the tooth, in order to transmit vibration to surrounding bone. The variations in bone micro-architecture were computed by image processing analyzed with samples prepared in transparent resin, allowing the measurement of morphometry before and after the induced changes in mineral content. Using vibrational bone response, impedance measurements allowed to observe the variations in bone mass as the samples were progressively decalcified. In the 5-50kHz spectrum, it was demonstrated the sensitivity of the electro-mechanical impedance during the bone alteration procedure since the electrical resistance signals of the piezo-device consistently changed in the frequency spectrum (5-50kHz). The piezo-device shows to be sensitive to the changes produced by the bone alterations, which were caused by the stiffness variations made in the sample during the decalcifying. These changes were statistically correlated to demonstrate that in a less invasive way, bone alterations could be monitored from the teeth. This result opens the door to search for a new way to diagnose bone density changes in real applications.
