Correction to Bioanalysis of Eukaryotic Organelles

Satori, Chad P.; Henderson, Michelle M.; Krautkramer, Elyse A.; Košťál, Vratislav; Distefano, Mark D.; Arriaga, Edgar A.

doi:10.1021/cr400254d

Cited by 2 publications

(2 citation statements)

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“…Using this technique, several thousand diverse molecular species have been identified in various cellular compartments5678. However, molecular profiling with mass-spectroscopy involves cell fractionation and extraction of biomolecules from various organelles, which inherently produces artifacts and is not compatible with live systems9. At the same time, conventional live cell imaging techniques, such as fluorescence microscopy, can identify only a few molecular species at a time, which significantly limits their efficiency for comprehensive molecular characterization of single cells and subcellular structures10111213.…”

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confidence: 99%

Resonance Raman Probes for Organelle-Specific Labeling in Live Cells

Kuzmin

Pliss

Lim

et al. 2016

Sci Rep

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Raman microspectroscopy provides for high-resolution non-invasive molecular analysis of biological samples and has a breakthrough potential for dissection of cellular molecular composition at a single organelle level. However, the potential of Raman microspectroscopy can be fully realized only when novel types of molecular probes distinguishable in the Raman spectroscopy modality are developed for labeling of specific cellular domains to guide spectrochemical spatial imaging. Here we report on the design of a next generation Raman probe, based on BlackBerry Quencher 650 compound, which provides unprecedentedly high signal intensity through the Resonance Raman (RR) enhancement mechanism. Remarkably, RR enhancement occurs with low-toxic red light, which is close to maximum transparency in the biological optical window. The utility of proposed RR probes was validated for targeting lysosomes in live cultured cells, which enabled identification and subsequent monitoring of dynamic changes in this organelle by Raman imaging.

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confidence: 99%

Resonance Raman Probes for Organelle-Specific Labeling in Live Cells

Kuzmin

Pliss

Lim

et al. 2016

Sci Rep

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“…Indeed, the human body is made of trillions of cells each with their own specific functionality . Each cell is made up of cellular organelles, such as the plasma membrane, mitochondria, lysosomes, Golgi apparatus, and endoplasmic reticulum (ER) . Though it has long been known that these structures are vital for cellular operation and consequently, the whole body, further study has revealed more detailed understanding of their significance pertaining to specific biochemical processes and pathways .…”

Section: Introductionmentioning

confidence: 99%

Red AIE‐Active Fluorescent Probes with Tunable Organelle‐Specific Targeting

Alam

Leung

et al. 2020

Adv Funct Materials

104

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Cell staining is a fascinating research area where monitoring and visualizing different cell organelles can be done using fluorescence techniques. However, the design and synthesis of organelle‐targeting fluorophores is still a challenge for several specific organelles. Herein, a platform for synthesizing efficient red‐emitting aggregation‐induced emission luminogens (AIEgens) with donor–acceptor characteristics is reported. The core molecule can be easily functionalized in order to modulate organelle targeting. The three synthesized AIEgens exhibit quantum yields of up to 39.3% and two‐photon absorption cross‐section values of up to 162 GM. The two zwitterionic AIEgens, CDPP‐3SO3 and CDPP‐4SO3, with the sulfonate function group, are successfully utilized for specific one‐photon and two‐photon imaging of the endoplasmic reticulum (ER) in live human cells. Substituting the zwitterionic nature with a singly positive charge group, one‐photon and two‐photon imaging of CDPP‐BzBr shows mitochondrial specificity, indicating the importance of the zwitterionic group for ER‐targeting. Owing to the good in vitro photostability, cell viability, and high efficiency, these red dyes serve as a good potential candidate for specific organelle targeting, as well as illustrate how such a platform can easily aid in the study of structure–property relationships for designing such probes.

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Correction to Bioanalysis of Eukaryotic Organelles

Cited by 2 publications

References 0 publications

Resonance Raman Probes for Organelle-Specific Labeling in Live Cells

Resonance Raman Probes for Organelle-Specific Labeling in Live Cells

Red AIE‐Active Fluorescent Probes with Tunable Organelle‐Specific Targeting

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