Correction to: Comparison of efficiency and time to regeneration of Agrobacterium-mediated transformation methods in Medicago truncatula

Wen, Li; Chen, Yuanling; Schnabel, Elise; Crook, Ashley D.; Frugoli, Julia

doi:10.1186/s13007-019-0480-2

Cited by 2 publications

(2 citation statements)

References 3 publications

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“…Previous studies have pointed out that phosphinothricin (PPT) selection (phosphinothricin acetyltransferase gene, Bar) brought more stringent and rapid selection than Kan selection (Larkin et al 1996;Wen et al 2019), and the e ciency of transgenic recovery using PPT was superior when compared with Kan (RamanaRao and Veluthambi 2010). We need to spend more labor and time to monitor the white clover tissue constantly, though some white clover escapes and chimeras died during the long period of Kan selection.…”

Section: Comparison Of Two Transformationmentioning

confidence: 99%

Two Agrobacterium-mediated Transformation Protocols of White Clover (Trifolium Repens) Through the Callus System

Jia

Cheng

Feng

et al. 2022

Preprint

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White clover (Trifolium repens) is one of the most widely cultivated livestock forage legumes co-cultivated worldwide with pasture grass in a mixed-sward setting, however, its persistence and aesthetic quality are severely affected by abiotic stresses. In this study, regeneration of white clover plants was conducted through a callus system for 4-5 months with a regeneration frequency of 36-41%. Inoculating 4-day-old cotyledons into MS media fortified with 0.4 mg·L-1 6-BA and 2 mg·L-1 2,4-D significantly increased the callus formation rate. Roots and cotyledons were better induced, followed by hypocotyls, leaves, and petioles. The development of differentiated structures performed effectively on MS supplemented with 1 mg·L-1 6-BA and 0.1 mg·L-1 NAA. Further, we determined factors affecting the Agrobacterium tumefaciens-mediated transient transformation for root-derived callus and 4-day-old cotyledons. The parameters that facilitated transient transformation were: Agrobacterium suspension density of 0.5 (OD600), 20 mg·L-1 AS, and 4-days co-cultivation duration. Subsequently, we developed two transformation protocols: transformation after callus formation in root segments (Protocol A) and transformation before callus initiation in 4-day-old cotyledons (Protocol B). The transformation frequencies varied from 1.92% to 3.17% in Protocol A and from 2.76% to 3.47% in Protocol B. We offer the possibility to regenerate multiple transgenic white clover from a single genetic background. In addition to assistance in identification of functional genes associated with yield, resistance and aesthetic quality, our research will also contribute to successful genetic manipulation and genome editing in white clover.

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Section: Comparison Of Two Transformationmentioning

confidence: 99%

Two Agrobacterium-mediated Transformation Protocols of White Clover (Trifolium Repens) Through the Callus System

Jia

Cheng

Feng

et al. 2022

Preprint

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“…Tissue culture is often an important limitation in the generation of transgenic events or the efficiency of transformation since it requires a great deal of professional experience to perform (Altpeter et al, 2016). The use of specific transformation protocols or tissue culture procedures for each strategy or plant genotype is required (Wen et al, 2019). Recalcitrant genotypes require a culture medium suitable for each step of induction, cocultivation, selection or regeneration to prevent tissue oxidation and Agrobacterium inhibition and to enhance selection and plant regeneration.…”

Section: Plant Tissue Culturementioning

confidence: 99%

Insights Into Genetic and Molecular Elements for Transgenic Crop Development

et al. 2020

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Climate change and the exploration of new areas of cultivation have impacted the yields of several economically important crops worldwide. Both conventional plant breeding based on planned crosses between parents with specific traits and genetic engineering to develop new biotechnological tools (NBTs) have allowed the development of elite cultivars with new features of agronomic interest. The use of these NBTs in the search for agricultural solutions has gained prominence in recent years due to their rapid generation of elite cultivars that meet the needs of crop producers, and the efficiency of these NBTs is closely related to the optimization or best use of their elements. Currently, several genetic engineering techniques are used in synthetic biotechnology to successfully improve desirable traits or remove undesirable traits in crops. However, the features, drawbacks, and advantages of each technique are still not well understood, and thus, these methods have not been fully exploited. Here, we provide a brief overview of the plant genetic engineering platforms that have been used for proof of concept and agronomic trait improvement, review the major elements and processes of synthetic biotechnology, and, finally, present the major NBTs used to improve agronomic traits in socioeconomically important crops.

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Correction to: Comparison of efficiency and time to regeneration of Agrobacterium-mediated transformation methods in Medicago truncatula

Cited by 2 publications

References 3 publications

Two Agrobacterium-mediated Transformation Protocols of White Clover (Trifolium Repens) Through the Callus System

Two Agrobacterium-mediated Transformation Protocols of White Clover (Trifolium Repens) Through the Callus System

Insights Into Genetic and Molecular Elements for Transgenic Crop Development

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