Correction to Crystal Growth of Lysozyme Controlled by Laser Trapping

Abstract

On page 16, left column, second paragraph, first line, of this paper, the notation of sample of HEWL (Wako, >99.0%) is incorrect. The correct notation is as follows: HEWL (Wako, for Biochemistry).

Dynamics and Mechanism of Laser Trapping-Induced Crystal Growth of Hen Egg White Lysozyme

“…enhancement. We here should point out that the trend of growth rate change shown in Figure 4 is fully consistent with that in our previous work, 18 and these results are completely different than those on spontaneous growth with constant growth rate. Since such a considerably large SD for the mean G 101 and G 110 was still observed in this work, we carefully checked their temporal changes for each sample individually.…”

“…For temperature elevation by laser heating, we have already reported that little or no effect of the temperature elevation contributes to the crystal growth rate. 18 Furthermore, we also confirmed in this study that temperature elevation of a glass substrate by laser irradiation is negligible for the crystal growth rate. We carried out the same trapping experiment by replacing a glass substrate with a fused quartz slide showing a much lower absorption coefficient at 1064 nm.…”

“…In this study, only a tetragonal HEWL crystal of 10−20 μm in length and with an L/W ratio of 1.5 ± 0.1 was selected as a starting crystal for our laser trapping experiments, whose experimental conditions are stricter than those in our previous study. 18 The images of the growing crystal during laser irradiation was captured for every 5 min by a CCD camera and then were analyzed for calculating G 101 and G 110 with ImageJ software. 22 For laser irradiation conditions, we set the laser focus to a point 10 μm away from {101} or {110} HEWL crystal faces, which is hereafter abbreviated as "{101} irradiation" or "{110} irradiation", respectively.…”

“…17 Recently, we applied the laser trapping-induced crystal growth method to a hen egg-white lysozyme (HEWL) solution. 18 We focused a CW NIR trapping laser beam at a position 10 μm away from the edge of a target HEWL crystal generated spontaneously and examined temporal changes in growth rate of {101} and {110} crystal faces. The growth behavior was completely different from that of spontaneous growth, which was explained by the mechanism as involving the formation of a highly concentrated domain of HEWL liquidlike clusters.…”

A Single Large Assembly with Dynamically Fluctuating Swarms of Gold Nanoparticles Formed by Trapping Laser

“…40−42 By control of the laser polarization, the resultant crystal morphology is biased to certain forms. 41,42 The protein growth rate of lysozyme can be controlled by laser trapping, 43,44 amyloid fibril formation of cytochrome C can be spatiotemporally induced by laser trapping. 45 In general, the nanoparticles and molecules are trapped, concentrated at the focus, and distributed outside of the focus, giving their clusters, assemblies, and even crystals.…”