Correction to: Proximity ligation scaffolding and comparison of two Trichoderma reesei strains genomes

Jourdier, Etienne; Baudry, Lyam; Poggi-Parodi, Dante; Vicq, Yoan; Koszul, Romain; Margeot, Antoine; Marbouty, Martial; Bidard, Frédérique

doi:10.1186/s13068-018-1161-5

Cited by 2 publications

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“…Trichoderma reesei , the work horse for cellulase production in industry, contains 11 genes encoding β-glucosidases which are classified into glycoside hydrolase (GH) families 1 ( cel1a and cel1b ) and 3 ( cel3a , cel3b , cel3c , cel3d , cel3e , cel3f , cel3g , cel3h , and cel3j ) on the basis of their sequence identity and structural similarity [ 19 , 20 ]. They are distributed at different genomic sites with genes cel3e , cel3f and cel3g in chromosome I, cel1b in chromosome II, cel3a , cel1a and cel3d in chromosome III, cel3h , cel3j and cel3c in chromosome IV, and cel3b in chromosome VI [ 21 ]. Their effects on cellulase production, and their cellular distribution and secretion have been comprehensively studied, identifying that β-glucosidases CEL1A, CEL3B, CEL3E, CEL3F, CEL3G, CEL3H, and CEL3J are extracellular, CEL3C and CEL3D intracellular, CEL1B unknown [ 22 ].…”

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confidence: 99%

Transmembrane transport process and endoplasmic reticulum function facilitate the role of gene cel1b in cellulase production of Trichoderma reesei

Pang

Luo

et al. 2022

Microb Cell Fact

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Background A total of 11 β-glucosidases are predicted in the genome of Trichoderma reesei, which are of great importance for regulating cellulase biosynthesis. Nevertheless, the relevant function and regulation mechanism of each β-glucosidase remained unknown. Results We evidenced that overexpression of cel1b dramatically decreased cellulase synthesis in T. reesei RUT-C30 both at the protein level and the mRNA level. In contrast, the deletion of cel1b did not noticeably affect cellulase production. Protein CEL1B was identified to be intracellular, being located in vacuole and cell membrane. The overexpression of cel1b reduced the intracellular pNPGase activity and intracellular/extracellular glucose concentration without inducing carbon catabolite repression. On the other hand, RNA-sequencing analysis showed the transmembrane transport process and endoplasmic reticulum function were affected noticeably by overexpressing cel1b. In particular, some important sugar transporters were notably downregulated, leading to a compromised cellular uptake of sugars including glucose and cellobiose. Conclusions Our data suggests that the cellulase inhibition by cel1b overexpression was not due to the β-glucosidase activity, but probably the dysfunction of the cellular transport process (particularly sugar transport) and endoplasmic reticulum (ER). These findings advance the knowledge of regulation mechanism of cellulase synthesis in filamentous fungi, which is the basis for rationally engineering T. reesei strains to improve cellulase production in industry.

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