Correction to: Refactoring of a synthetic raspberry ketone pathway with EcoFlex

In synthetic biology, biosensors are routinely coupled to a gene expression cascade for detecting small molecules and physical signals. We posit that an alternative direct protein (DiPro) biosensor mechanism, could provide a new opportunity for rapid detection of specific chemicals. Herein, we reveal a fluorescent curcumin DiPro biosensor, based on the Escherichia coli double bond reductase (EcCurA) as a detection system. We characterise the curcumin DiPro biosensor and propose enhanced fluorescence is generated through π-π stacking between protein and ligand. Using a cell-free synthetic biology approach, we use the DiPro biosensor to fine-tune 10 reaction parameters (cofactor, substrate, and enzyme levels), assisted through acoustic liquid handling robotics. Overall, we increase curcumin DiPro biosensor fluorescence by 80-fold. We propose a generic DiPro biosensor fluorescence mechanism that can be further exploited for a wider range of chemicals that share intrinsic fluorescence and have a suitable binding protein.

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A curcumin direct protein (DiPro) biosensor for cell-free prototyping

Lesniewska

Griffin

Freemont

et al. 2021

Preprint

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Correction to: Refactoring of a synthetic raspberry ketone pathway with EcoFlex

Cited by 1 publication

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A curcumin direct protein (DiPro) biosensor for cell-free prototyping

A curcumin direct protein (DiPro) biosensor for cell-free prototyping

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