“…Previous studies have used lumogallion staining and fluorescence microscopy for the detection and visualization of Al within paraffin-embedded tissues and viable cells. ,− This relative quantitative method can help to understand the distribution of Al within samples, but it cannot absolutely quantify Al or replace ICP–MS/MS quantification. Limitations of this staining method are the fact that lumogallion only binds to free Al 3+ but not to complexed Al, as it would be present in food color lake pigments.…”