We recently developed a database for hexaploid wheat QTL (WheatQTLdb; www.wheatqtldb.net) that included 11,552 QTL affecting various traits of economic importance. However, that database did not include valuable QTL from other wheat species and/or progenitors of bread wheat. Therefore, an updated and wider version of wheat QTL database (WheatQTLdb V2.0) was developed in this study which now includes information on hexaploid wheat (Triticum aestivum) as well as seven other related species: T. durum, T. turgidum, T. dicoccoides, T. dicoccum, T. monococcum, T. boeoticum, and Aegilops tauschii. WheatQTLdb V2.0 includes a much improved list of QTL, so that V2.0 now has 27,518 main-effect QTL, 202 epistatic QTL, and 1,321 metaQTL. This newly released WheatQTLdb V2.0 will provide plant breeders and geneticists much more valuable options to search and choose the category-wise and trait-wise data for their research or breeding programmes.