Correction: Toda, K., et al. Key Factors Affecting Strength Development of Steel Slag-Dredged Soil Mixtures. Minerals 2018, 8(5), 174

Abstract

The authors wish to make the following corrections to this paper [...]

Repressor sequestration activates translation of germ granule localized mRNA

“…In the PGCs of zebrafish embryos, nanos3 mRNA is suggested to be translated at the periphery of germ granules based on the distribution of ribosomes 75 . Pou5f3 mRNA granules in zebrafish embryos also have been shown to colocalize with nascent Pou5f3 peptides, suggesting the granules as translation sites 18 . In mouse spermatids, liquid-liquid phase separation of FXR1 is essential for translational activation of FXR1 target mRNAs, suggesting that FXR1-RNA condensates are the compartments for activated translation 17 .…”

“…The basic assumptions of using FRAP experiments to calculate translation elongation rate have been discussed previously [16][17][18] . We assume that 1) ribosomes are uniformly distributed within the open reading frame (ORF); 2) ribosome elongation rate is constant; 3) scFv-GCN4 epitope binding is stable and exchange at a significantly slower rate than translational elongation (44); 4) nascent peptides are immediately released when synthesis completes.…”

“…The rationale and mathematical basis of calculating ribosome occupancy using fluorescence of polysomes and single SunTag protein is based on previous studies [16][17][18][19][20][21] . To measure ribosome occupancy, we used rabbit anti-GCN4 antibody to detect the SunTag peptides in embryos from Vasa-mApple; suntag-nanos flies, which provides high signal-to-noise ratio and allows clear visualization of single fully-synthesized SunTag-Nanos protein (Extended Data Fig.…”

“…Recently, evidence has emerged that condensation of specific RBP via liquid-liquid phase separation can activate the translation of their target mRNAs 17 . Several studies have reported that specific cytoplasmic RNP granules may serve as translation factories [18][19][20][21] . However, the role of RNP granules in translational activation remains unclear.…”