Correlação entre métodos de monitoramento de limpeza e desinfecção de superfícies ambulatoriais

Abstract

Objectives: To evaluate the correlation among microbiological culture, ATP bioluminescence assay, and visual inspection in monitoring the effectiveness of surface cleaning and disinfection in an outpatient facility and determine the ATP bioluminescence cutoff capable of indicating a clean surface regarding microbiological evaluation. Methods: Exploratory, cross-sectional, and correlation study consisting of 720 evaluations in fi ve surfaces before and after cleaning and disinfection. The results were used to run two-proportions tests, calculate Spearman's correlation, and plot the receiver operating characteristic curve. Results: Similar proportions (p≥0.05) occurred for non-approval rates between ATP-bioluminescence and aerobic colony count only when the evaluations of all the surfaces before and after cleaning and disinfection were put together. There was a signifi cant correlation between the ATP quantifi cation and microbial count methods for the reception desk and the stretcher. Receiver operating characteristic analysis indicated that ATP quantifi cation showed a signifi cant result in comparison with aerobic colony count (p=0.044). Conclusion: There was a discrete correlation between the ATP quantifi cation and microbial count methods for two surfaces. It is suggested that surfaces showing values ≤49 relative light units are clean. ResumoObjetivo: Avaliar a correlação entre cultura microbiológica, teste de ATP por bioluminescência e inspeção visual na monitorização da efi ciência da limpeza e da desinfecção de superfícies de uma unidade ambulatorial e determinar o valor de corte de ATP-bioluminescência capaz de indicar superfície limpa em relação à avaliação microbiológica. Métodos: Estudo exploratório, longitudinal e correlacional. Foram realizadas 720 avaliações em cinco superfícies antes e após a limpeza e a desinfecção. Nos resultados, foram realizadas análises de duas proporções, a correlação de Spearman e a curva ROC. Resultados: Ocorreram proporções semelhantes (p≥0,05) entre as taxas de reprovação apenas entre ATP-bioluminescência e contagem de colônias aeróbias (CCA) quando somadas as avaliações de todas as superfícies antes e depois da limpeza e da desinfecção. Houve correlação signifi cativa entre os métodos de quantifi cação de ATP e a contagem microbiana para o balcão da recepção e a maca. A análise ROC indicou que a quantifi cação de ATP apresentou resultado signifi cativo na comparação com a CCA (p=0,044). Conclusão: Embora discreta, houve correlação signifi cativa entre os métodos de quantifi cação de ATP e contagem microbiana para duas superfícies. Sugere-se que superfícies que apresentam valores ≤49 unidades relativas de luz estão limpas. ResumenObjetivo: Analizar la correlación entre cultivo microbiológico, prueba de ATP por bioluminiscencia e inspección visual en el monitoreo de la efi ciencia de la limpieza y desinfección de superfi cies en una unidad ambulatoria y determinar el valor de referencia de ATP-bioluminiscencia que indique que la superfi cie está limpia con relación a la eva...

Impact of Educational Intervention on Cleaning and Disinfection of an Emergency Unit

“…The collections were performed twice a week, assessing the surfaces before and after the cleaning and nursing team performed the C&D, that is, four assessments before cleaning and four assessments after cleaning, totaling eight assessments per day and 16 per week for each method. The study consisted of three stages: Stage 1-baseline (4 weeks of collection), Stage 2-intervention (4 weeks of collection) and immediate assessment and Stage 3 (4 weeks of collection)-long-term assessment (two months after the educational intervention performed in Stage 2) [18][19][20][21]. There was a total of 192 evaluations for each phase, considering the three methods.…”

“…The educational intervention for individuals was based on other studies [7,18,19]. We used as educational method one training with expository dialogue, which was carried out in two periods (morning and afternoon), for 1 h and 30 min.…”

“…Therefore, the educational intervention was more than a training; it was also a standardization of practices, sanitizers and supplies that are used. In other words, in Stage 2, the team was aware of the objectives of the study and which surfaces were being monitored, and even received feedback during the collections [7,8,11,19].…”

“…In order to assess the long-term impact, the same procedures of Stage 1 were also performed in this stage. At this stage, no feedback was provided to the team [7,8,11,19].…”

Biofilms formation and prevention on urinary catheters: a bibliometric analysis

“…Health care-related infection (HCI) has a direct impact on the safety and quality of health care. It may involve the individual in institutions of hospital and / or outpatient care, as a day or home hospital and may be associated with some assistance procedure (therapeutic or diagnostic) (FURLAN et al, 2019a;MATOS et al, 2018;FURLAN et al, 2019b;SOUSA et al, 2017b).…”