Correlated variation of chiasma frequency and synaptonemal complex length in Locusta migratoria

Quevedo, C; Cerro, A. L. del; Santos, J. L.; Jones, G H

doi:10.1038/sj.hdy.6881320

Cited by 10 publications

(14 citation statements)

References 15 publications

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“…This statement can now be extended to higher plants, since we obtained comparable results in A. thaliana. Such correlated variations in SC length and CO number were also reported among chromosomes in one sex only, among individuals in the same species, and even among meiocytes in a single organism [5,17,[47][48][49][50]. However, based on current knowledge it is difficult to claim that SC length determines CO number, if the reverse is true, or even if another unidentified factor determines both.…”

Section: Discussionmentioning

confidence: 83%

Sex-specific crossover distributions and variations in interference level along Arabidopsis thaliana chromosome 4

Drouaud¹,

Mercier²,

Chelysheva³

et al. 2005

PLoS Genet

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In many species, sex-related differences in crossover (CO) rates have been described at chromosomal and regional levels. In this study, we determined the CO distribution along the entire Arabidopsis thaliana Chromosome 4 (18 Mb) in male and female meiosis, using high density genetic maps built on large backcross populations (44 markers, .1,300 plants). We observed dramatic differences between male and female map lengths that were calculated as 88 cM and 52 cM, respectively. This difference is remarkably parallel to that between the total synaptonemal complex lengths measured in male and female meiocytes by immunolabeling of ZYP1 (a component of the synaptonemal complex). Moreover, CO landscapes were clearly different: in particular, at both ends of the map, male CO rates were higher (up to 4-fold the mean value), whereas female CO rates were equal or even below the chromosomal average. This unique material gave us the opportunity to perform a detailed analysis of CO interference on Chromosome 4 in male and female meiosis. The number of COs per chromosome and the distances between them clearly departs from randomness. Strikingly, the interference level (measured by coincidence) varied significantly along the chromosome in male meiosis and was correlated to the physical distance between COs. The significance of this finding on the relevance of current CO interference models is discussed.

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Section: Discussionmentioning

confidence: 83%

Sex-specific crossover distributions and variations in interference level along Arabidopsis thaliana chromosome 4

Drouaud¹,

Mercier²,

Chelysheva³

et al. 2005

PLoS Genet

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“…One factor that has, however, been shown to influence the rate of meiotic recombination is the length of particular chromosome pairs in different cells. In the spermatocytes of grasshoppers and humans, measurement of chiasma positions along bivalents revealed that the lengths of these bivalents varied between cells and that this variation was positively correlated with chiasma frequency (Fox, 1973;Hultén, 1974;Quevedo et al, 1997). These earlier studies, however, were somewhat indirect as recombination could only be measured by chiasma analysis, i.e.…”

Section: Genomic Levels Of Recombinationmentioning

confidence: 94%

Inter-sex variation in synaptonemal complex lengths largely determine the different recombination rates in male and female germ cells

Tease

Hultén²

2004

Cytogenet Genome Res

138

106

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Meiotic chromosomes in human oocytes are packaged differently than in spermatocytes at the pachytene stage of meiosis I, when crossing-over takes place. Thus the meiosis-specific pairing structure, the synaptonemal complex (SC), is considerably longer in oocytes in comparison to spermatocytes. The aim of the present study was to examine the influence of this length factor on meiotic recombination in male and female human germ cells. The positions of crossovers were identified by the DNA mismatch repair protein MLH1. Spermatocytes have approximately 50 crossovers per cell in comparison to more than 70 in oocytes. Analyses of inter-crossover distances (and presumptively crossover interference) along SCs suggested that while there might be inter-individual variation, there was no consistent difference between sexes. Thus the higher rate of recombination in human oocytes is not a consequence of more closely spaced crossovers along the SCs. The rate of recombination per unit length of SC is higher in spermatocytes than oocytes. However, when the so-called obligate chiasma is excluded from the analysis, then the rates of recombination per unit length of SC are essentially identical in the two sexes. Our analyses indicate that the inter-sex difference in recombination is largely a consequence of the difference in meiotic chromosome architecture in the two sexes. We propose that SC length per se, and therefore the size of the physical platform for crossing-over (and not the DNA content) is the principal factor determining the difference in rate of recombination in male and female germ cells. A preliminary investigation of SC loop size by fluorescence in situ hybridization (FISH) indicated loops may be shorter in oocytes than in spermatocytes.

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“…In fact, this phenomenon has been previously noticed by other authors in human and other organisms. 8,10,21 Recently, a covariation of SC length has been described. 9 By comparing two different sets of chromosomes, one with chromosomes displaying different physical size but similar genetic length (chromosomes 16 and 19) and the other with chromosomes of similar physical size but different genetic length (chromosomes 21 and 21), it has been found that SC length reflects genetic length instead of physical length of a concrete chromosome.…”

Section: Resultsmentioning

confidence: 99%

Karyotyping of human synaptonemal complexes by cenM-FISH

et al. 2003

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The purpose of this work was to adapt the recently described centromere-specific multicolour (cenM-) FISH technique to human meiotic cells, and evaluate the usefulness of this multiplex fluorescence method for karyotyping human synaptonemal complex (SC), previously analysed by immunocytogenetic approaches. The results obtained demonstrate that cenM-FISH is a reliable one-single-step method, which allows for the identification of all SC present in pachytene spreads. Moreover, when cenM-FISH is applied after immunocytogenetic analysis, the number and distribution of MLH1 foci per chromosome can be established and recombination analysis for each chromosome can be performed easily.

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Correlated variation of chiasma frequency and synaptonemal complex length in Locusta migratoria

Cited by 10 publications

References 15 publications

Sex-specific crossover distributions and variations in interference level along Arabidopsis thaliana chromosome 4

Sex-specific crossover distributions and variations in interference level along Arabidopsis thaliana chromosome 4

Inter-sex variation in synaptonemal complex lengths largely determine the different recombination rates in male and female germ cells

Karyotyping of human synaptonemal complexes by cenM-FISH

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