Correlates of the molecular vaginal microbiota composition of African women

Gautam, Raju; Borgdorff, Hanneke; Jespers, Vicky; Francis, Suzanna C.; Verhelst, Rita; Mwaura, Mary; Delany‐Moretlwe, Sinead; Ndayisaba, Gilles; Kyongo, Jordan K.; Hardy, Liselotte; Menten, Joris; Crucitti, Tania; Tsivtsivadze, Evgeni; Schuren, Frank; Wijgert, Janneke HHM van de

doi:10.1186/s12879-015-0831-1

Cited by 46 publications

(55 citation statements)

References 36 publications

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“…Supporting these results, it has also been reported in other studies about the composition of species in the vaginal microbiome of black and white women born in the US, a significant difference between these two groups, in which black women have a greater microbial diversity and a lower probability of lactobacilli colonization than white women [24,79]. In other studies, conducted in sub-Saharan African countries, a smaller proportion of L. crispatus in the vaginal communities compared to women of European or Asian descent has been found [24,79,80]. Here, African communities were dominated by L. iners and by a variable mixture of facultative anaerobic bacteria [24,68,81].…”

Section: Ethnicitysupporting

confidence: 60%

Reviewing the Composition of Vaginal Microbiota: Inclusion of Nutrition and Probiotic Factors in the Maintenance of Eubiosis

et al. 2020

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The vaginal microbiota has importance in preserving vaginal health and defending the host against disease. The advent of new molecular techniques and computer science has allowed researchers to discover microbial composition in depth and associate the structure of vaginal microbial communities. There is a consensus that vaginal flora is grouped into a restricted number of communities, although the structure of the community is constantly changing. Certain Community-State Types (CSTs) are more associated with poor reproductive outcomes and sexually transmitted diseases (STDs) meanwhile, CSTs dominated by Lactobacillus species—particularly Lactobacillus crispatus—are more related to vaginal health. In this work, we have reviewed how modifiable and non-modifiable factors may affect normal vaginal microbiota homeostasis—including sexual behavior, race or ethnicity, and hygiene. Special interest has been given to how the use of probiotics, diet intake, and use of hormone replacement therapies (HRTs) can potentially impact vaginal microbiota composition.

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Section: Ethnicitysupporting

confidence: 60%

Reviewing the Composition of Vaginal Microbiota: Inclusion of Nutrition and Probiotic Factors in the Maintenance of Eubiosis

et al. 2020

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“…20 Our cytokine findings are consistent with previous studies that reported an increase of cervicovaginal pro-inflammatory cytokines in women with BV. 11,13,14 However, some cytokines that have frequently been studied in this context (such as IL-1b, IL-6, and IL-8) were not identified in our study, probably due to relatively low concentrations that fall outside the dynamic range of the mass spectrometer. 41 The cytokines that we did detect (IL-36a, IL-36g, Il-36 receptor antagonist, migration inhibitory factor, glucose-6-phosphate isomerase, C5, metalloproteinase inhibitor 1) have not been studied in the context of vaginal dysbiosis before.…”

Section: Articlesmentioning

confidence: 59%

“…However, recent clinical studies have shown increased cervicovaginal fluid concentrations of the pro-inflammatory cytokines interleukin (IL)-1b and IL-8, and decreased concentrations of the antiinflammatory cytokine IL-10 and the antiproteases secretory leukocyte protease inhibitor and elafin (PI3). [9][10][11][12][13][14] Furthermore, in vitro studies have shown that lactobacilli do not generally induce human AMPs, whereas BV-associated bacteria (such as Gardnerella vaginalis, A. vaginae and P. bivia) do. 10,12 The cervicovaginal proteome has been characterized by others before, [15][16][17][18][19][20] but never in the context of different VMB compositions.…”

Section: Introductionmentioning

confidence: 99%

Cervicovaginal microbiome dysbiosis is associated with proteome changes related to alterations of the cervicovaginal mucosal barrier

et al. 2016

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Vaginal microbiome (VMB) dysbiosis is associated with increased acquisition of HIV. Cervicovaginal inflammation and other changes to the mucosal barrier are thought to have important roles but human data are scarce. We compared the human cervicovaginal proteome by mass spectrometry of 50 Rwandan female sex workers who had previously been clustered into four VMB groups using a 16S phylogenetic microarray; in order of increasing bacterial diversity: Lactobacillus crispatus-dominated VMB (group 1), Lactobacillus iners-dominated VMB (group 2), moderate dysbiosis (group 3), and severe dysbiosis (group 4). We compared relative protein abundances among these VMB groups using targeted (abundance of pre-defined mucosal barrier proteins) and untargeted (differentially abundant proteins among all human proteins identified) approaches. With increasing bacterial diversity, we found: mucus alterations (increasing mucin 5B and 5AC), cytoskeleton alterations (increasing actin-organizing proteins; decreasing keratins and cornified envelope proteins), increasing lactate dehydrogenase A/B as markers of cell death, increasing proteolytic activity (increasing proteasome core complex proteins/proteases; decreasing antiproteases), altered antimicrobial peptide balance (increasing psoriasin, calprotectin, and histones; decreasing lysozyme and ubiquitin), increasing proinflammatory cytokines, and decreasing immunoglobulins immunoglobulin G1/2. Although temporal relationships cannot be derived, our findings support the hypothesis that dysbiosis causes cervicovaginal inflammation and other detrimental changes to the mucosal barrier.

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“…In 2010–2011, we conducted a multi-country follow-up study entitled ‘‘Characterisation of novel microbicide safety biomarkers in East and South Africa”. The main aim of that project was to characterise the vaginal microbiome and the cervicovaginal mucosal immune system in African women and to assess changes of these over time [ 18 – 21 ]. In that study, 430 women were recruited at three study sites, i.e.…”

Section: Methodsmentioning

confidence: 99%

A Multi-Country Cross-Sectional Study of Vaginal Carriage of Group B Streptococci (GBS) and Escherichia coli in Resource-Poor Settings: Prevalences and Risk Factors

et al. 2016

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BackgroundOne million neonates die each year in low- and middle-income countries because of neonatal sepsis; group B Streptococcus (GBS) and Escherichia coli are the leading causes. In sub-Saharan Africa, epidemiological data on vaginal GBS and E. coli carriage, a prerequisite for GBS and E. coli neonatal sepsis, respectively, are scarce but necessary to design and implement prevention strategies. Therefore, we assessed vaginal GBS and E. coli carriage rates and risk factors and the GBS serotype distribution in three sub-Saharan countries.MethodsA total of 430 women from Kenya, Rwanda and South Africa were studied cross-sectionally. Vaginal carriage of GBS and E. coli, and GBS serotype were assessed using molecular techniques. Risk factors for carriage were identified using multivariable logistic regression analysis.ResultsVaginal carriage rates in reference groups from Kenya and South Africa were 20.2% (95% CI, 13.7–28.7%) and 23.1% (95% CI, 16.2–31.9%), respectively for GBS; and 25.0% (95% CI, 17.8–33.9%) and 27.1% (95% CI, 19.6–36.2%), respectively for E. coli. GBS serotypes Ia (36.8%), V (26.3%) and III (14.0%) were most prevalent. Factors independently associated with GBS and E. coli carriage were Candida albicans, an intermediate vaginal microbiome, bacterial vaginosis, recent vaginal intercourse, vaginal washing, cervical ectopy and working as a sex worker. GBS and E. coli carriage were positively associated.ConclusionsReduced vaginal GBS carriage rates might be accomplished by advocating behavioral changes such as abstinence from sexual intercourse and by avoidance of vaginal washing during late pregnancy. It might be advisable to explore the inclusion of vaginal carriage of C. albicans, GBS, E. coli and of the presence of cervical ectopy in a risk- and/or screening-based administration of antibiotic prophylaxis. Current phase II GBS vaccines (a trivalent vaccine targeting serotypes Ia, Ib, and III, and a conjugate vaccine targeting serotype III) would not protect the majority of women against carriage in our study population.

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Correlates of the molecular vaginal microbiota composition of African women

Cited by 46 publications

References 36 publications

Reviewing the Composition of Vaginal Microbiota: Inclusion of Nutrition and Probiotic Factors in the Maintenance of Eubiosis

Reviewing the Composition of Vaginal Microbiota: Inclusion of Nutrition and Probiotic Factors in the Maintenance of Eubiosis

Cervicovaginal microbiome dysbiosis is associated with proteome changes related to alterations of the cervicovaginal mucosal barrier

A Multi-Country Cross-Sectional Study of Vaginal Carriage of Group B Streptococci (GBS) and Escherichia coli in Resource-Poor Settings: Prevalences and Risk Factors

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