Correlating 13C Isotope in Oligomeric Proanthocyanidins with their Anticancer Properties

Abstract

Upon considering the anticancer effects of larger oligomeric proanthocyanidins and observing various papers reporting the high resolution mass spectroscopy of the oligomeric proanthocyanidins, it is determined that the unusual 13C enrichment in some plant oligomeric proanthocyanidins may be responsible for the anticancer activities of these food products. Such correlation of the 13C in the oligomeric proanthocyanidins also correlate with their scavenging of free-radicals, anti-virial and anti-bacterial properties. Proanthocyanidins in grape seeds are observed to have high enrichment in heavy isotopes of 2H, 13C, 15N and/or 17O. Mass analysis of DNA from human cancer cells are compared to normal human cells and cancer cells show bond specific enrichment of heavy isotopes in nucleotides G, A, T and C. On such basis, this study suggests possible stronger interactions of proanthocyanidins with DNA in cancer verses DNA in normal cells due to heavy isotope bond specific enrichments in both proanthocyanidins and the cancer DNA. Such 13C interactions from oligomeric proanthocyanidins with nucleic acids and proteins involved in replications, transcriptions and translations in cancer cells for interacting and chemically altering anabolism and cell division of the cancer cells are consistent with the author’s mechanism for normal cell to cancer cell transformations via possible replacements of primordial 1H, 12C, 14N, 16O, and 24Mg isotopes by nonprimordial 2H, 13C, 15N, and 17O and 25Mg isotopes in the proteins and nucleic acids. Such is also consistent with the proposed treatment for cancer by the author by use of foods containing proteins, nucleic acids, carbohydrates and/or drug molecules enriched with the nonprimordial isotopes of 2H, 13C, 15N, and 17O and 25Mg.

Relativistic Chiral Inversion of Non-Zero Nuclear Magnetic Moments During Centrifugal Industrial Fermentative Processes

“…The author strengthens his theory of NMMs of 1 H, 14 N, and 31 P organizing replication, transcription and translation by comparing his theory to the recently observed alteration of protein translation by some protists by disclosing in this work the protists' inclination to enrich their carbohydrates, proteins and DNA and RNA with 13 C, 15 N, 17 O and 18 O for 18 O induced alteration of DNA and RNA to 18 O enriched G * and T * having induced +NMM of 18 O for G * behaving as A and T * behaving as A for explaining the misreading of aspartic acid and glutamine STOPs and lysine and glutamic acid and the altered genetic expressions of some protists. On such basis, the author proposes alterations of normal biochemical reactions for causing disease, aging, and eventual death by clumpings and accumulations of trace, uncommon stable isotopes of 2 D (smaller + 0.857 NMM), 13 C (+ 0.702 NMM), 15 N (− 0.283 NMM), 17 O (− 1.894 NMM), 18 O (induced + NMM), 25 Mg (− 0.855 NMM), 26 Mg (induced + NMM), 33 S (+ 0.644 NMM), 40 K (− 1.298 NMM), 41 K (induced -NMM), and/or 43 Ca (− 1.317 NMM) relative to more commonly [primordial amounts found in normal, healthy cells], stable isotopes of 1 H (induced negative NMM), 12 C (+NMM), 14 N (+ 0.404 NMM), 16 O (zero NMM), 24 Mg (0 NMM), 32 S (0 NMM), 39 K (+ 0.391 NMM), and/or 40 Ca (0 NMM). The author demonstrates in this work the altered gravity by displacements in space and even accelerated motions (centrifugation) on earth for inducing replacement of common stable isotopes of these elements by their corresponding less common nonprimordial stable isotopes with consequent different biochemistry for changes in metabolism for explain diseases like cancer, diabetes, Alzheimer's disease and more.…”

“…The isolations from humans, animals, and plants are more expensive on industrial scales. The productions by isolations from organic sources as humans, plants, and animals better preserve the isotopic distributions of 2 D, 13 C, 15 N, 17 O, 25 Mg and 33 S and other isotopes of trace minerals, as organic sources preserve the intrinsic homochirality of biomolecules in living organisms. However, the chemical syntheses on industrial scales more often lead to formations of racemic (50%-50%) mixtures of the 2 isomers (D and L enantiomers) of these biomolecules for explaining why ultra-processed foods are not healthy.…”

“…Furthermore, the author notes the presence of quanta having nonzero nuclear magnetic moments (NMMs) heightens these effects and induces such effects under milder less energetic conditions. On such basis, the author noted astronauts in space away from earth fit this model as their transportations to outer space changes the gravity on them and the recycling of their waste of urine (enriched with 15 N) for water and CO 2 (enriched with 13 C and 17 O) conversions to sugars provide diets enriched in 13 C, 15 N and 17 O under severely altered gravity for altering the motions and isotopic enrichments in their bodies and consequent altered molecular recognitions [3]- [5]. Such changes in motion and gravity on the body of the astronauts change the biochemical reactions in their bodies and possibly induce nuclear reactions in their bodies under cosmic ray interactions.…”

“…The author further hypothesizes that simulated altered gravity during the centrifugations in biophysical technologies employing centrifugations of bacterial and fungal cultures having ingredients of sugar and urine of known high 13 C, 15 N and 17 O enrichments leads to altered stereochemistry and inversions about chiral centers of amino acids and sugars and nucleic acids. Sugar and urine used in many industrial fermentative processes are known to be enriched in 13 C, 15 N, and 17 O as these come from C4 plants [6] and from human and animal wastes.…”

“…The author further hypothesizes that simulated altered gravity during the centrifugations in biophysical technologies employing centrifugations of bacterial and fungal cultures having ingredients of sugar and urine of known high 13 C, 15 N and 17 O enrichments leads to altered stereochemistry and inversions about chiral centers of amino acids and sugars and nucleic acids. Sugar and urine used in many industrial fermentative processes are known to be enriched in 13 C, 15 N, and 17 O as these come from C4 plants [6] and from human and animal wastes. The accelerations by the centrifugations are hypothesized here to cause altered gravity to simulate either stronger gravity on different planets, say Jupiter or Saturn, and/or to simulate reduction in gravity as displacing the organisms from the surface of earth to satellite orbiting earth in free fall for such altered gravity to alter chirality and alter metabolisms.…”