Correlating Cardiac Structure to Function Using Nanoscale Resolution Scanning Ion Conductance Microscopy

Bhargava, Anamika; Gorelik, Julia

doi:10.1007/11663_2021_10

Cited by 2 publications

(3 citation statements)

References 37 publications

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“…This was done so that minute changes in the myofilament spacing could be observed. 7. There is a possibility that environmental factors can affect the myofilament d-spacing in the most immature tissue; if so, then this effect will be most observable at the tissue's extrema.…”

Section: Discussionmentioning

confidence: 99%

“…Given the importance of the sarcomeric organization of cardiac tissue, methods for structural characterization are particularly critical to this effort. Scientists and engineers have developed optical methods [2][3][4], electron microscopy (EM) techniques [2,5,6], and other approaches [7][8][9] to characterize sarcomeric organization, but these tools for nanoscale measurement of intact tissue are limited to information from at most a few tens of microns from the surface, resulting in incomplete characterization of the subcellular structure.…”

Section: Introductionmentioning

confidence: 99%

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Structural maturation of myofilaments in engineered 3D cardiac microtissues characterized using small angle x-ray scattering

van Dover,

Javor,

Ewoldt

et al. 2024

Phys. Biol.

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Understanding the structural and functional development of human-induced pluripotent stem-cell-derived cardiomyocytes is essential to engineering cardiac tissue that enables pharmaceutical testing, modeling diseases, and designing therapies. Here we use a method not commonly applied to biological materials, small angle X-ray scattering, to characterize the structural development of human-induced pluripotent stem-cell-derived cardiomyocytes within 3D engineered tissues during their preliminary stages of maturation. An X-ray scattering experimental method enables the reliable characterization of the cardiomyocyte myofilament spacing with maturation time. The myofilament lattice spacing monotonically decreases as the tissue matures from its initial post-seeding state over the span of ten days. Visualization of the spacing at a grid of positions in the tissue provides an approach to characterizing the maturation and organization of cardiomyocyte myofilaments and has the potential to help elucidate mechanisms of pathophysiology, and disease progression, thereby stimulating new biological hypotheses in stem cell engineering.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Structural maturation of myofilaments in engineered 3D cardiac microtissues characterized using small angle x-ray scattering

van Dover,

Javor,

Ewoldt

et al. 2024

Phys. Biol.

View full text Add to dashboard Cite

show abstract

“…Given the importance of the sarcomeric organization of cardiac tissue, methods for structural characterization is particularly important to this effort. Scientists and engineers have developed optical methods [2][3][4] , electron microscopy (EM) techniques 2,5,6 , and other approaches [7][8][9] to characterize sarcomeric organization, but tools for nanoscale measurement of intact tissue are limited to information from at most a few tens of microns from the surface, resulting in incomplete characterization of the subcellular structure.…”

Section: Introductionmentioning

confidence: 99%

Structural maturation of myofilaments in engineered 3D cardiac microtissues characterized using small angle X-ray scattering

Javor

Ewoldt

Zhernenkov

et al. 2023

Preprint

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Understanding the structural and functional development of human-induced pluripotent stem-cell-derived cardiomyocytes is essential to engineering cardiac tissue that enables pharmaceutical testing, modeling diseases, and designing therapies. Here, we used a method not commonly applied to biological materials, small angle X-ray scattering to characterize the structural development of human induced pluripotent stem-cell-derived cardiomyocytes within 3D engineered tissues during their preliminary stages of maturation. An innovative X-ray scattering experimental setup enabled the visualization of a systematic variation in the cardiomyocyte myofilament spacing with maturation time. The myofilament lattice spacing monotonically decreased as the tissue matured from its initial post-seeding state over the span of ten days. Visualization of the spacing at a grid of positions in the tissue provides a new approach to characterizing the maturation and organization of cardiomyocyte myofilaments and has the potential to help elucidate mechanisms of pathophysiology, disease progression, thereby stimulating new biological hypotheses in stem cell engineering.

show abstract

Correlating Cardiac Structure to Function Using Nanoscale Resolution Scanning Ion Conductance Microscopy

Cited by 2 publications

References 37 publications

Structural maturation of myofilaments in engineered 3D cardiac microtissues characterized using small angle x-ray scattering

Structural maturation of myofilaments in engineered 3D cardiac microtissues characterized using small angle x-ray scattering

Structural maturation of myofilaments in engineered 3D cardiac microtissues characterized using small angle X-ray scattering

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