Correlating measured transient temperature rises with damage rate processes in cultured cells

Denton, Michael L.; Tijerina, Amanda J.; Gonzalez, Cherry C.; Gamboa, Bruno; Noojin, Gary D.; Ahmed, Elharith M.; Rickman, John M.; Dyer, Phillip H.; Rockwell, Benjamin A.

doi:10.1117/12.2250916

Cited by 1 publication

(2 citation statements)

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“…In addition to the delivery of the laser, the microscope served to image (L4 and L1) and record video (60 fps) of cell samples, and image (L2 and L1) and record microthermography. A Plexiglas enclosure chamber 49,[51][52][53] provided consistent ambient temperature and 60%-75% relative humidity. As measured by microthermography, the average ambient temperature for all 91 individual 1-s and 3-s exposures was 34.3°C AE 0.3°C.…”

Section: Methodsmentioning

confidence: 99%

“…Both laser delivery and microthermography were carried out from beneath the cells in a similar manner to that described, 53 where the final imaging optic prior to cell samples was a 75-mm CaF 2 lens (CF-PX-38-75, ISP Optics, Corp., Irvington, New York). Figure 3 provides a schematic of the experimental set up for the variable ambient temperature experiment.…”

Section: Cells Without Pigment (2-μm Exposures)mentioning

confidence: 99%

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Effect of ambient temperature and intracellular pigmentation on photothermal damage rate kinetics

et al. 2019

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Computational models predicting cell damage responses to transient temperature rises generated by exposure to lasers have implemented the damage integral (Ω), which time integrates the chemical reaction rate constant described by Arrhenius. However, few published reports of empirical temperature histories (thermal profiles) correlated with damage outcomes at the cellular level are available to validate the breadth of applicability of the damage integral. In our study, an analysis of photothermal damage rate processes in cultured retinal pigment epithelium cells indicated good agreement between temperature rise, exposure duration (τ), and threshold cellular damage. Full-frame thermograms recorded at high magnification during laser exposures were overlaid with fluorescence damage images taken 1 h postexposure. From the image overlays, pixels of the thermogram correlated with the boundary of cell death were used to extract threshold thermal profiles. Assessing photothermal responses at these boundaries standardized all data points, irrespective of laser irradiance, damage size, or optical and thermal properties of the cells. These results support the hypothesis that data from boundaries of cell death were equivalent to a minimum visible lesion, where the damage integral approached unity (Ω ¼ 1) at the end of the exposure duration. Empirically resolved Arrhenius coefficients for use in the damage integral determined from exposures at wavelengths of 2 μm and 532 nm and durations of 0.05-20 s were consistent with literature values. Varying ambient temperature (T amb) between 20°C and 40°C during laser exposure did not change the τ-dependent threshold peak temperature (T p). We also show that, although threshold laser irradiance varied due to pigmentation differences, threshold temperatures were irradiance independent.

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Section: Methodsmentioning

confidence: 99%

Section: Cells Without Pigment (2-μm Exposures)mentioning

confidence: 99%