Correlation Analysis of Relative Expression of Apob, Adfp and Fatp1 with Lipid Metabolism in Daweishan Mini Chickens

Abstract

Quantitative RT-PCR was applied to measure the relative expression levels of the adipose differentiation-related protein (ADFP) gene, fatty acid transport protein 1 (FATP1) gene and apolipoprotein B (ApoB) gene in subcutaneous fat, abdominal fat, liver and muscle at five growth stages (28, 49, 70, 91 and 112 d) to determine the effect of the expression ofthese genes on fat deposition in Daweishan Mini chickens. The relative expression of ADFP gene mRNA in the abdominal fat andthe liver was significantly different between 49 d and 70 d (p<0.05). The relative ApoB gene expression on 91d was higher in the liver, followed by muscles,subcutaneous fat, and abdominal fat, and was significantly higher in the liver than in the other three tissues. FATP1 gene expression in the liver presented a significant positive correlation with subcutaneous fat thickness (p<0.05). The results of this study suggest that the three genes may control the fat development in Daweishan Mini chicken.

Variations in Tissue-Specific Expression of Adipose Differentiation-Related Protein Gene in Two Native Yunnan Chicken Breeds

“…The results of the present study showed that the ADFP gene is expressed in the abdominal fat, subcutaneous fat, liver and pectoralis muscle of both PC and WBC, which is consistent with previous research (Decai et al, 2017;Brasaemle et al, 1997;Zhao et al, 2010). In addition, ADFP gene expression level peaked in PC on d 70 in the liver, subcutaneous fat and pectoralis muscle, whereas in WBC, the highest ADFP expression levels in abdominal fat, liver, and pectoralis muscle were determined on d 91, 112, and 28, respectively.…”

“…Although many research studies have been devoted to the regulation of the ADFP gene in mammals, to the best of our knowledge, no studies on the role of the ADFP gene in Yunnan Native chicken breeds have been published, except for the research of Decai et al (2017). The objective of the present study was to detect the tissue-specific expression of the ADFP gene in Piao chickens (PC) and Wuliangshan black-bone chickens (WBC) in order elucidate its role in fat deposition in two Yunnan native chicken breeds.…”

“…On five different days of the grow-out period, at days 28, 49, 70, 91, and 112 days of age, 12 individuals of each breed (six males and six females) were slaughtered, and the pectoralis muscle, liver, abdominal fat and subcutaneous fat were collected according to the sampling method of Decai et al (2017) and stored in liquid nitrogen at 80ºC. Live weight (LW), backfat thickness (BFT) (on the back midline and anterior to the uropygial gland) and comb weight (CW) were determined (Table 1).…”

“…In this study, for the evaluation of relative ADFP gene expression, the b-actin gene was used as internal control. The primers used for ADFP mRNA (GenBank accession: NM_001031420.1) and b-actinmRNA (GenBank accession: NM_205518.1) amplification sequences are reported elsewhere (Decai et al, 2017). All primer sequences were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China)…”

“…Ontogenetic expression and tissue-differential expression of the ADFP gene were analyzed with Duncan's test of SPSS 18 software in two Yunnan Native chicken breeds. Correlation analysis between ADFP mRNA expression levels and CF were calculated using the Pearson method according to Decai et al (2017). P-values lower than 0.05 were considered significant.…”