Correlation and Mechanism Study of Multivoxel 1H-MRS Parameters, Cho / Cr and CHKA

Abstract

Background Glioma is a common malignant brain tumor with strong invasiveness and poor prognosis. in order to improve the survival rate of patients, there is an urgent need to find the targeted molecular mechanism affecting its invasion and development. Methods In this study, the differential regions of Cho/Cr metabolism of tumor cells were identified by multi-voxel 1H-MRS and the tumor tissues of Cho/Cr high metabolism group and Cho/Cr low metabolism group were accurately obtained by combining neuronavigation system. The expression of CHKA in different Cho/Cr metabolic groups was verified by Real-timePCR and Western-blot. Then we used CHKA inhibitor MN58b and short hairpin RNA to study the role of CHKA in the proliferation and invasion of U251 glioma cells, and the relationship between CHKA and EMT in nude mice.Results We found that the expression of CHKA in Cho/Cr high metabolism group was significantly higher than that in Cho/Cr low metabolism group. Decreased CHKA activity can inhibit the proliferation and invasion of glioma cells and slow down the process of EMT.Conclusion CHKA may be an oncogenic gene, which affects the invasion and development of glioma cells by regulating the process of tumor EMT, and is expected to become a molecular target for glioma therapy, and multi-voxel 1H-MRS parameter Cho/Cr may become an important clinical index for detecting CHKA expression.