Correlation between antibiotic susceptibilities and genotypes in Neisseria gonorrhoeae from different geographical origins: determinants monitoring by real-time PCR as a complementary tool for surveillance

Vernel-Pauillac, Frédérique; Ratsima, Elisoa; Guillard, Bertrand; Goursaud, R; Lethezer, Camille; Hem, Sopheak; Mérien, Fabrice; Goarant, Cyrille

doi:10.1136/sti.2009.038000

Cited by 8 publications

(8 citation statements)

References 29 publications

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“…Staphylococcus aureus was the most frequently cultured organism and the majority (22/23; 95.7%) of isolates were meticillin sensitive, although with the emergence of community-acquired meticillin-resistant S. aureus (MRSA) infections in Cambodia this may change with time [15]. Isolates of Neisseria gonorrhoeae were penicillin and fluoroquinolone resistant but susceptible to ceftriaxone, which is similar to the findings of other eye infection studies from Southeast Asia [16, 17]. The addition of molecular testing increased the spectrum of detectable pathogens, most notably in neonates where 60% of infections were associated with detection of Chlamydia trachomatis DNA.…”

Section: Resultssupporting

confidence: 68%

Ophthalmic infections in children presenting to Angkor Hospital for Children, Siem Reap, Cambodia

et al. 2014

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BackgroundOphthalmic infections cause significant morbidity in Cambodian children but aetiologic data are scarce. We investigated the causes of acute eye infections in 54 children presenting to the ophthalmology clinic at Angkor Hospital for Children, Siem Reap between March and October 2012.FindingsThe median age at presentation was 3.6 years (range 6 days – 16.0 years). Forty two patients (77.8%) were classified as having an external eye infection, ten (18.5%) as ophthalmia neonatorum, and two (3.7%) as intra-ocular infection. Organisms were identified in all ophthalmia neonatorum patients and 85.7% of patients with an external eye infection. Pathogens were not detected in either of the intra-ocular infection patients. Most commonly isolated bacteria were Staphylococcus aureus (23 isolates), coagulase-negative staphylococci (13), coliforms (7), Haemophilus influenzae/parainfluenzae (6), Streptococcus pneumoniae (4), and Neisseria gonorrhoeae (2). Chlamydia trachomatis DNA was detected in 60% of swabs taken from ophthalmia neonatorum cases.ConclusionsThis small study demonstrates the wide range of pathogens associated with common eye infections in Cambodian children. The inclusion of molecular assays improved the spectrum of detectable pathogens, most notably in neonates.

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Section: Resultssupporting

confidence: 68%

Ophthalmic infections in children presenting to Angkor Hospital for Children, Siem Reap, Cambodia

et al. 2014

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“…In contrast, AST data was reported by one paper for Shigella spp. (Meng et al, 2011), and two papers for N. gonorrhoeae (Khauv et al, 2014;Vernel-Pauillac et al, 2010). Four studies reported isolates from nonhuman populations, specifically, E. coli and NTS.…”

Section: Resultsmentioning

confidence: 99%

“…Two papers reported AST data for N. gonorrhoeae (Supplementary Table S7). One reported ciprofloxacin (93.3%) and penicillin (100%) resistance rates on 15 isolates from Cambodia, as part of a larger multi-country study (Vernel-Pauillac et al, 2010). The other reported AST data for 3GC (0%) and ciprofloxacin (100%) from two isolates (Khauv et al, 2014).…”

Section: Neisseria Gonorrhoeaementioning

confidence: 99%

Antimicrobial resistance in Cambodia: a review

Reed

Krang

Miliya

et al. 2019

International Journal of Infectious Diseases

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Objectives: Following the launch of the Global Antimicrobial Resistance Surveillance System (GLASS), antimicrobial resistance (AMR) rates in many countries remain poorly described. This review provides an overview of published AMR data from Cambodia in the context of recently initiated national human and food-animal surveillance. Methods: PubMed and the Cochrane Database of Systematic Reviews were searched for articles published from 2000 to 2018, which reported antimicrobial susceptibility testing (AST) data for GLASS specific organisms isolated from Cambodia. Articles were screened using strict inclusion/exclusion criteria. AST data was extracted, with medians and ranges of resistance rates calculated for specific bug-drug combinations. Results: Twenty-four papers were included for final analysis, with 20 describing isolates from human populations. Escherichia coli was the most commonly described organism, with median resistance rates from human isolates of 92.8% (n = 6 articles), 46.4% (n = 4), 55.4% (n = 8), and 46.4% (n = 5) to ampicillin, 3 rd generation cephalosporins, fluoroquinolones, and gentamicin respectively. Conclusions: Whilst resistance rates are high for several GLASS organisms, there were insufficient data to draw robust conclusions about the AMR situation in Cambodia. The recently implemented national AMR surveillance systems will begin to address this data gap.

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“…In Cambodia, antimicrobial resistance is a growing concern which has already been documented for Neisseria gonorrhoeae [21], S. aureus [22], Salmonella [23,24], Shigella [23], and Escherichia coli [25]. Nearly 45% of the 20 S. aureus strains isolated by the Kampong Cham Hospital laboratory in 2011 were community acquiredmethicillin resistant S. aureus (MRSA) (Somary N., Personal communication, 2011).…”

Section: Discussionmentioning

confidence: 99%

Surveillance of post-caesarean surgical site infections in a hospital with limited resources, Cambodia

Srun

Yin

Seng

et al. 2013

J Infect Dev Ctries

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Introduction: In Cambodia, we implemented a pilot surveillance of superficial surgical site infections (SSSI) following caesarean deliveries (CD) in a provincial hospital, to estimate their incidence, describe their clinical management, and determine their causative pathogens. Methodology: Between October 2010 and February 2011, all women admitted for CD were included in the surveillance. Their clinical condition was monitored for a post-operative period of 30 days, including two assessments performed by surgeons. Cases were clinically diagnosed by surgeons, with bacterial cultures performed. Results: Of the 222 patients admitted for CD, 176 (79.3%) were monitored for 30 days. Of these, 11 were diagnosed with a SSSI, giving an incidence rate of 6.25% (95% CI 3.2-10.9). Four of the cases (36.4%) were detected after hospital discharge. Length of hospitalization was significantly longer for the SSSI cases. All 222 patients were prescribed antibiotics. Ampicillin was administered intravenously to 98.6% of them, with subsequent oral amoxicillin given to 82.9%. Three of six pus samples collected were positive on culture: two with Staphylococcus aureus and one with Staphylococcus lugdunensis. One S.aureus was methicillin resistant (MRSA). The other was clindamycin and erythromycin resistant. Conclusion: Surveillance of health-care associated infections in a setting with limited resources is challenging but feasible. Effective postdischarge surveillance was essential for the estimation of the incidence rate of SSSI following caesarean deliveries. This surveillance led to a peer-review of medical practices.

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Correlation between antibiotic susceptibilities and genotypes in Neisseria gonorrhoeae from different geographical origins: determinants monitoring by real-time PCR as a complementary tool for surveillance

Cited by 8 publications

References 29 publications

Ophthalmic infections in children presenting to Angkor Hospital for Children, Siem Reap, Cambodia

Ophthalmic infections in children presenting to Angkor Hospital for Children, Siem Reap, Cambodia

Antimicrobial resistance in Cambodia: a review

Surveillance of post-caesarean surgical site infections in a hospital with limited resources, Cambodia

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