Correlation between Biophysical Properties of Niosomes Elaborated with Chloroquine and Different Tensioactives and Their Transfection Efficiency

Sainz-Ramos, Myriam; Villate-Beitia, Ilia; Gallego, Idoia; Qtaish, Nuseibah Al; Menéndez, Margarita; Lagartera, Laura; Grijalvo, Santiago; Eritja, Ramón; Puras, Gustavo; Pedraz, José Luís

doi:10.3390/pharmaceutics13111787

Cited by 8 publications

(3 citation statements)

References 79 publications

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“…Slightly high viscosity was recorded at very low shear rates, which can be related to the fact that relatively large niosomes offer resistance (although low) to start flowing. The obtained viscosity values were in good agreement with those recorded in previous reports [49,50].…”

Section: Niosome Characterizationsupporting

confidence: 91%

Formulation and Characterization of Epalrestat-Loaded Polysorbate 60 Cationic Niosomes for Ocular Delivery

et al. 2023

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The aim of this work was to develop niosomes for the ocular delivery of epalrestat, a drug that inhibits the polyol pathway and protects diabetic eyes from damage linked to sorbitol production and accumulation. Cationic niosomes were made using polysorbate 60, cholesterol, and 1,2-di-O-octadecenyl-3-trimethylammonium propane. The niosomes were characterized using dynamic light scattering, zeta-potential, and transmission electron microscopy to determine their size (80 nm; polydispersity index 0.3 to 0.5), charge (−23 to +40 mV), and shape (spherical). The encapsulation efficiency (99.76%) and the release (75% drug release over 20 days) were measured with dialysis. The ocular irritability potential (non-irritating) was measured using the Hen’s Egg Test on the Chorioallantoic Membrane model, and the blood glucose levels (on par with positive control) were measured using the gluc-HET model. The toxicity of the niosomes (non-toxic) was monitored using a zebrafish embryo model. Finally, corneal and scleral permeation was assessed with the help of Franz diffusion cells and confirmed with Raman spectroscopy. Niosomal permeation was higher than an unencapsulated drug in the sclera, and accumulation in tissues was confirmed with Raman. The prepared niosomes show promise to encapsulate and carry epalrestat through the eye to meet the need for controlled drug systems to treat the diabetic eye.

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Section: Niosome Characterizationsupporting

confidence: 91%

Formulation and Characterization of Epalrestat-Loaded Polysorbate 60 Cationic Niosomes for Ocular Delivery

et al. 2023

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“…The m-RNA vaccination against coronavirus demonstrated the safety and efficiency of non-viral delivery systems for nucleic acid-based agents [ 346 , 347 , 348 ]. The lipid composition is not trivial and contains synthetic molecules, such as PEG-lipids and lipid-amine molecules.…”

Section: Recent Progress In Non-viral Gene Therapymentioning

confidence: 99%

Gene Therapy for Regenerative Medicine

et al. 2023

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The development of biological methods over the past decade has stimulated great interest in the possibility to regenerate human tissues. Advances in stem cell research, gene therapy, and tissue engineering have accelerated the technology in tissue and organ regeneration. However, despite significant progress in this area, there are still several technical issues that must be addressed, especially in the clinical use of gene therapy. The aims of gene therapy include utilising cells to produce a suitable protein, silencing over-producing proteins, and genetically modifying and repairing cell functions that may affect disease conditions. While most current gene therapy clinical trials are based on cell- and viral-mediated approaches, non-viral gene transfection agents are emerging as potentially safe and effective in the treatment of a wide variety of genetic and acquired diseases. Gene therapy based on viral vectors may induce pathogenicity and immunogenicity. Therefore, significant efforts are being invested in non-viral vectors to enhance their efficiency to a level comparable to the viral vector. Non-viral technologies consist of plasmid-based expression systems containing a gene encoding, a therapeutic protein, and synthetic gene delivery systems. One possible approach to enhance non-viral vector ability or to be an alternative to viral vectors would be to use tissue engineering technology for regenerative medicine therapy. This review provides a critical view of gene therapy with a major focus on the development of regenerative medicine technologies to control the in vivo location and function of administered genes.

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“…Size, zeta potential and polydispersity determination. The hydrodynamic diameter, which includes particle size, reported as mean particle intensity, and the polydispersity index (PDI) of nanocarriers were measured by Dynamic Light Scattering (DLS), and the Zeta potential was measured by Lasser Doppler Velocimetry (LDV) in a Zetasizer Nano ZS (Malvern Instrument, UK) 54 . To carry out the measurements, 50 µL of each sample was diluted in 950 µL of 0.1 mM NaCl solution.…”

Section: Thermogravimetric Analysis (Tga)mentioning

confidence: 99%

Design of double functionalized carbon nanotube for amphotericin B and genetic material delivery

Yazdani

Mozaffarian

Pazuki

et al. 2022

Sci Rep

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In the present work, single wall carbon nanotubes (SWCNT) were successively functionalized with phospholipid DSPE-PEG carboxylic acid, and then, with ethylenediamine (EDA), to obtain double functionalized single wall carbon nanotube (DFSWCNT). Then, DFSWCNT was applied as a carrier for delivering amphotericin B (Amb) and EGFP plasmid. FSWCNT’s concentration obtained via UV–visible analysis was 0.99 mg/mL. The TGA analysis results provided the lost weights of DSPE-PEG-COOH, EDA, Amb and SWCNT impurities. XPS results showed that carbon atoms’ percentage decreased during the functionalization processes from 97.2% (SWCNT) to 76.4% (FSWCNT) and 69.9% (DFSWNCT). Additionally, the oxygen atoms’ percentage increased from 2.3% (SWCNT) to 21% and 22.5% for FSWCNT and DFSWCNT, respectively. New bonds such as C–N and N–C=O appeared in the synthesized nanocarrier. The IG/ID ratio in Raman analysis decreased from 7.15 (SWCNT) to 4.08 (FSWCNT). The amount of Amb released to phosphate buffer saline medium was about 33% at pH = 5.5 and 75% at pH = 7.4 after 48 h. CCK8 results confirmed that the toxicity of functionalized SWCNT had decreased. In a 2:1 ratio of DFSWCNT/EGFP plasmid, the cell viability (87%) and live transfected cells (56%) were at their maximum values. The results indicate that carbon nanotubes have the potential to be applied as drug/gene delivery systems with outstanding properties such as high loading capacity and easy penetration to cell membrane.

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Correlation between Biophysical Properties of Niosomes Elaborated with Chloroquine and Different Tensioactives and Their Transfection Efficiency

Cited by 8 publications

References 79 publications

Formulation and Characterization of Epalrestat-Loaded Polysorbate 60 Cationic Niosomes for Ocular Delivery

Formulation and Characterization of Epalrestat-Loaded Polysorbate 60 Cationic Niosomes for Ocular Delivery

Gene Therapy for Regenerative Medicine

Design of double functionalized carbon nanotube for amphotericin B and genetic material delivery

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