Correlation between cellular fluorescence and NAD(P)H levels in Alcaligenes eutrophus JMP 134

NADH is a coenzyme which plays a central role in cellular growth and metabolism. It is an intracellular fluorophore which fluoresces at 460 nm when cells are irradiated by 340 nm wavelength of light. The application of NADH+H(+) fluorescence measurement for characterization of biomass and its metabolic activity during batch fermentation of 1,3-propanediol (1,3-PD) using Clostridium diolis was investigated in this study. A linear correlation between net fluorescence and biomass concentration was observed during both the initial and final phases of 1,3-PD fermentation. This could be used as an on-line indicator of biomass concentration inside the bioreactor thereby eliminating the need for sampling and off-line analysis for establishing biomass concentration during these phases. Also a sharp decline in the NADH+H(+) fluorescence value was obtained towards the end of fermentation which could be a significant on-line, in situ signal of substrate depletion in the bioreactor and therefore possible fresh nutrient feed for enhanced production of 1,3-PD by repetitive and/or various fed-batch cultivation(s). This is the first report on the use of NADH + H(+) fluorescence measurement technique for 1,3-PD fermentation.

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Rapid extraction of (di)nucleotides from bacterial cells and determination by ion-pair reversed-phase HPLC

Müller

Loffhagen

Babel

1996

Journal of Microbiological Methods

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Correlation between cellular fluorescence and NAD(P)H levels in Alcaligenes eutrophus JMP 134

Cited by 4 publications

References 21 publications

Substrate inhibition under stationary growth conditions - nutristat experiments with Ralstonia eutropha JMP 134 during growth on phenol and 2,4-dichlorophenoxyacetate

Substrate inhibition under stationary growth conditions - nutristat experiments with Ralstonia eutropha JMP 134 during growth on phenol and 2,4-dichlorophenoxyacetate

On-line Characterization of Metabolic State in Batch Cultivation of Clostridium diolis for 1,3-Propanediol Production Using NADH+H+ Fluorescence

Rapid extraction of (di)nucleotides from bacterial cells and determination by ion-pair reversed-phase HPLC

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